Roles of the microRNA‑338‑3p/NOVA1 axis in retinoblastoma

Sun, Shoubin; Wang, Runze; Yi, Sisi; Li, Sijia; Wang, Lei; Wang, Jianwen

doi:10.3892/mmr.2021.12033

Cited by 7 publications

(4 citation statements)

References 31 publications

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“…All isomiR mimics also produced similar downregulation of endogenous Aatk mRNA (Supplementary Figure S3D). Finally, isomiR mimics failed to affect Nova1 mRNA, another known miR-338-3p target (Sun et al, 2021), but all resulted in NOVA1 protein downregulation (Supplementary Figures S3E-G). Together, these results suggest that the miR-338-3p FL, −G, and −UG isomiRs are functional in Neuro-2a cells, and we find no evidence that the 3′ trimming that produces the -G and -UG isomiRs interferes with miR-338-3p function.…”

Section: Resultsmentioning

confidence: 99%

MicroRNA 3′ ends shorten during adolescent brain maturation

Thomas

Vermare

Egleston

et al. 2023

Front. Mol. Neurosci.

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MicroRNA (miRNA) dysregulation is well-documented in psychiatric disease, but miRNA dynamics remain poorly understood during adolescent and early adult brain maturation, when symptoms often first appear. Here, we use RNA sequencing to examine miRNAs and their mRNA targets in cortex and hippocampus from early-, mid-, and late-adolescent and adult mice. Furthermore, we use quantitative proteomics by tandem mass tag mass spectrometry (TMT-MS) to examine protein dynamics in cortex from the same subjects. We found that ~25% of miRNAs’ 3′ ends shorten with age due to increased 3′ trimming and decreased U tailing. Particularly, shorter but functionally competent isoforms (isomiRs) of miR-338-3p increase up to 10-fold during adolescence and only in brain. MiRNAs that undergo 3′ shortening exhibit stronger negative correlations with targets that decrease with age and stronger positive correlations with targets that increase with age, than miRNAs with stable 3′ ends. Increased 3′ shortening with age was also observed in available mouse and human miRNA-seq data sets, and stronger correlations between miRNAs that undergo shortening and their mRNA targets were observed in two of the three available data sets. We conclude that age-associated miRNA 3′ shortening is a well-conserved feature of postnatal brain maturation.

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Section: Resultsmentioning

confidence: 99%

MicroRNA 3′ ends shorten during adolescent brain maturation

Thomas

Vermare

Egleston

et al. 2023

Front. Mol. Neurosci.

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“…Recently, NOVA1 had been reported to serve as downstream target gene for a series of microRNAs and was demonstrated to be responsible for the regulation of cellular biological behavior in cancer [25][26][27][28]. The inhibitory effect of microRNA miR-193a-5p on the PTEN/PI3k/ AKT pathway can be abrogated by NOVA1 in glioblastoma [29].…”

Section: Discussionmentioning

confidence: 99%

NOVA1 promotes NSCLC proliferation and invasion by activating Wnt/β-catenin signaling

Tian

Wang

et al. 2022

BMC Cancer

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Background Neuro-oncological ventral antigen 1 (NOVA1) is a neuron-specific RNA-binding protein which regulates alternative splicing in the developing nervous system. Recent research has found that NOVA1 plays a significant role in carcinogenesis. In this paper, we examine the role of NOVA1 in non-small cell lung cancer (NSCLC) and its underlying molecular mechanisms. Methods The expression of NOVA1 in NSCLC was detected by immunohistochemistry and correlations between NOVA1 expression and clinicopathological factors were analyzed by chi–square tests. Kaplan–Meier survival analysis and the Cox regression model were used to evaluate the predictive effect of prognostic factors. Western blotting, Cell Counting Kit-8, colony formation, apoptosis, migration and invasion assays were used to detect the effects of silencing (si)NOVA1 RNA on Wnt/β-catenin signaling and biological behavior in NSCLC cell lines. Results Our study showed that expression of NOVA1 was up-regulated and significantly correlated with poor differentiation (p = 0.020), advanced TNM stage (P = 0.001), T stage (P = 0.001) and lymph node metastasis (P = 0.000) as well as the expression of β-catenin (P = 0.012) in NSCLC. The down-regulation of NSCLC by siRNA significantly inhibited proliferation, migration and invasion and promoted apoptosis in NSCLC cells. Expression of Wnt signaling molecules, including β-catenin, activated β-catenin, cyclin D1, matrix metalloproteinase (MMP)-2 and MMP-7, was also significantly reduced by siNOVA1. The inhibition of Wnt/β-catenin signaling in A549 and H1299 cells by siNOVA1 was reversed after treatment with a β-catenin expression plasmid. Conclusion The present study suggests that NOVA1 may serve as a potential prognosis biomarker in NSCLC. High NOVA1 expression was associated with poor survival rate. Finally, in vitro experiments verified that NOVA1 promotes NSCLC cell proliferation and invasion by regulating Wnt/β-catenin signaling.

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“…For the TUNEL assay 28 , after routine dehydration, hydration, and repair of paraffin sections of each set of brain tissues, 100 µl of proteinase K (20 µg/ml) was added to each sample according to the instructions of the TUNEL kit and incubated for 30 min at room temperature. Then, 50 µl of TUNEL assay was added dropwise to the samples and incubated for 60 min at 37 °C, protected from light.…”

Section: Methodsmentioning

confidence: 99%

Treadmill training improves lung function and inhibits alveolar cell apoptosis in spinal cord injured rats

Wang,

Fu,

Yang

et al. 2024

Sci Rep

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Secondary lung injury after SCI is a major cause of patient mortality, with apoptosis playing a key role. This study aimed to explore the impact of treadmill training and miR145-5p on the MAPK/Erk signaling pathway and apoptosis in rats with complete SCI. SD rats were used to establish T10 segmental complete SCI models and underwent treadmill training 3, 7, or 14 days postinjury. Various techniques including arterial blood gas analysis, lung wet/dry weight ratio, HE staining, immunofluorescence staining, immunohistochemical staining, qRT-PCR, and Western blotting were employed to assess alterations in lung function and the expression levels of crucial apoptosis-related factors. In order to elucidate the specific mechanism, the impact of miR145-5p on the MAPK/Erk pathway and its role in apoptosis in lung cells were confirmed through miR145-5p overexpression and knockdown experiments. Following spinal cord injury (SCI), an increase in apoptosis, activation of the MAPK/Erk pathway, and impairment of lung function were observed in SCI rats. Conversely, treadmill training resulted in a reduction in alveolar cell apoptosis, suppression of the MAPK/Erk pathway, and enhancement of lung function. The gene MAP3K3 was identified as a target of miR145-5p. The influence of miR145-5p on the MAPK/Erk pathway and its impact on apoptosis in alveolar cells were confirmed through the manipulation of miR145-5p expression levels. The upregulation of miR145-5p in spinal cord injury (SCI) rats led to a reduction in MAP3K3 protein expression within lung tissues, thereby inhibiting the MAPK/Erk signaling pathway and decreasing apoptosis. Contrarily, rats with miR145-5p knockdown undergoing treadmill training exhibited an increase in miR145-5p expression levels, resulting in the inhibition of MAP3K3 protein expression in lung tissues, suppression of the MAPK/Erk pathway, and mitigation of lung cell apoptosis. Ultimately, the findings suggest that treadmill training may attenuate apoptosis in lung cells post-spinal cord injury by modulating the MAP3K3 protein through miR145-5p to regulate the MAPK/Erk signaling pathway.

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Roles of the microRNA‑338‑3p/NOVA1 axis in retinoblastoma

Cited by 7 publications

References 31 publications

MicroRNA 3′ ends shorten during adolescent brain maturation

MicroRNA 3′ ends shorten during adolescent brain maturation

NOVA1 promotes NSCLC proliferation and invasion by activating Wnt/β-catenin signaling

Treadmill training improves lung function and inhibits alveolar cell apoptosis in spinal cord injured rats

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