Roles of the CCR4‐Not complex in translation and dynamics of co‐translation events

Abstract

The Ccr4‐Not complex is a global regulator of mRNA metabolism in eukaryotic cells that is most well‐known to repress gene expression. Delivery of the complex to mRNAs through a multitude of distinct mechanisms accelerates their decay, yet Ccr4‐Not also plays an important role in co‐translational processes, such as co‐translational association of proteins and delivery of translating mRNAs to organelles. The recent structure of Not5 interacting with the translated ribosome has brought to light that embedded information within the codon sequence can be monitored by recruitment of the Ccr4‐Not complex to elongating ribosomes. Thereby, the Ccr4‐Not complex is empowered with regulatory decisions determining the fate of proteins being synthesized and their encoding mRNAs. This review will focus on the roles of the complex in translation and dynamics of co‐translation events.This article is categorized under: Translation > Mechanisms Translation > Regulation

Global Release of Translational Repression AcrossPlasmodium’sHost-to-Vector Transmission Event

“…Additionally, we detect proteins required by early mosquito-stage parasites to become motile (IMC proteins and their regulators, CelTOS), adjust their surface protein composition (p25, p28, PSOPs, CPW-WPCs, CTRP, WARP, all known members of the crystalloid), develop the ability to evade mosquito immunity (P47, Cap380), and prepare for segmentation (basal complex members). We also detected an increased abundance of NOT5, which plays a role in engaging translating ribosomes encountering non-optimal codons in the A-site (39). Moreover, we observed that key components of the mitochondria are differentially abundant, including much of the respiratory chain (Complexes III, IV, and ATP synthase), and associated transporters required for their function are differentially abundant in zygotes, reflecting the need to rapidly adapt to the lower nutrient availability of mosquitoes.…”