Roles of Three Transporters, CbcXWV, BetT1, and BetT3, in Pseudomonas aeruginosa Choline Uptake for Catabolism

Malek, Adel; Chen, Chyi‐Liang; Wargo, Matthew J.; Beattie, Gwyn A.; Hogan, Deborah A.

doi:10.1128/jb.00160-11

Cited by 43 publications

(72 citation statements)

References 48 publications

(50 reference statements)

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“…1). These data also support a model where initial uptake by BetT1 or BetT3 primes the induction of CbcXWV, creating a series of transport steps that can be regulated by choline and GB-sensitive transcription factors, as discussed below (48).…”

Section: Choline and Gb Import Versus De Novo Synthesissupporting

confidence: 56%

“…This section of the review focuses only on transport regulation by the osmolytes themselves, which occurs after the initial osmostress response and under nonstress conditions, and I refrain from review of sensing osmolarity and cell envelope stress, which has been well reviewed elsewhere (18,49,50). Choline and GB transporters in P. aeruginosa can be regulated at the transcriptional level (48). In most bacteria, one of the BCCT-family transporters is close to, and generally divergently transcribed from, the choline oxidase genes and its associated choline-responsive transcription factor (51-53).…”

Section: Choline and Gb Import Versus De Novo Synthesismentioning

confidence: 99%

“…The AraC family members canonically regulate genes involved in metabolism (e.g., AraC and XylS for regulation of arabinose and xylene/benzoate metabolism, respectively [59,60]) and/or virulence (61) (e.g., ExsA regulation of type III secretion in P. aeruginosa [62] and ToxT regulation of cholera toxin and the toxincoregulated pilus in Vibrio cholerae [63]). Evidence suggests that GbdR senses GB and dimethylglycine levels in the cell and induces transcription of genes involved in virulence, GB transport, GB catabolism, and detoxification of the catabolic byproducts, hydrogen peroxide and formaldehyde (48,56,(64)(65)(66)(67)(68) (Fig. 2).…”

Section: Choline and Gb Import Versus De Novo Synthesismentioning

confidence: 99%

“…There are strongly supported GbdR orthologues and orthologues of the entire P. aeruginosa choline degradation pathway in all other sequenced pseudomonads, suggesting similar capabilities across the genus. GbdR controls transcription of the cbcXWV operon in P. aeruginosa, thus stimulating increased transport as part of a positive-feedback loop (48). CbcW is the transmembrane-spanning subunit that assembles to form the transport pore, and CbcX is the In P. aeruginosa, choline and GB import are mediated by the BCCT-family transporters BetT1, BetT2, and BetT3 and the ABC-family transporter CbcWV, but the importance of each changes based on the external osmolarity.…”

Section: Choline and Gb Import Versus De Novo Synthesismentioning

confidence: 99%

“…The P. aeruginosa genome encodes three members of the BCCT (betaine-choline-carnitine transport) family and a single specific ABC (ATP binding cassette) family transporter predicted to be involved in quaternary amine transport. The BCCT family was largely thought to transport compatible solutes for the purpose of osmoprotection, while the ABC transporter has been shown to be important for bulk transport of choline as a primary carbon source (27,47,48). Recent work has elaborated the model of choline and GB uptake by P. aeruginosa, where it was shown that the four active transporters (BetT1, BetT2, BetT3, and CbcXWV) function to span the range of physiologic osmolarities to provide optimal uptake from the environment (48,117) (Fig.…”

Section: Choline and Gb Import Versus De Novo Synthesismentioning

confidence: 99%

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Homeostasis and Catabolism of Choline and Glycine Betaine: Lessons from Pseudomonas aeruginosa

Wargo

2013

Appl Environ Microbiol

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155

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Most sequenced bacteria possess mechanisms to import choline and glycine betaine (GB) into the cytoplasm. The primary role of choline in bacteria appears to be as the precursor to GB, and GB is thought to primarily act as a potent osmoprotectant. Choline and GB may play accessory roles in shaping microbial communities, based on their limited availability and ability to enhance survival under stress conditions. Choline and GB enrichment near eukaryotes suggests a role in the chemical relationships between these two kingdoms, and some of these interactions have been experimentally demonstrated. While many bacteria can convert choline to GB for osmoprotection, a variety of soil-and water-dwelling bacteria have catabolic pathways for the multistep conversion of choline, via GB, to glycine and can thereby use choline and GB as sole sources of carbon and nitrogen. In these choline catabolizers, the GB intermediate represents a metabolic decision point to determine whether GB is catabolized or stored as an osmo-and stress protectant. This minireview focuses on this decision point in Pseudomonas aeruginosa, which aerobically catabolizes choline and can use GB as an osmoprotectant and a nutrient source. P. aeruginosa is an experimentally tractable and ecologically relevant model to study the regulatory pathways controlling choline and GB homeostasis in choline-catabolizing bacteria. The study of P. aeruginosa associations with eukaryotes and other bacteria also makes this a powerful model to study the impact of choline and GB, and their associated regulatory and catabolic pathways, on host-microbe and microbe-microbe relationships.

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Section: Choline and Gb Import Versus De Novo Synthesissupporting

confidence: 56%

Section: Choline and Gb Import Versus De Novo Synthesismentioning

confidence: 99%

Section: Choline and Gb Import Versus De Novo Synthesismentioning

confidence: 99%

Section: Choline and Gb Import Versus De Novo Synthesismentioning

confidence: 99%

Section: Choline and Gb Import Versus De Novo Synthesismentioning

confidence: 99%

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Homeostasis and Catabolism of Choline and Glycine Betaine: Lessons from Pseudomonas aeruginosa

Wargo

2013

Appl Environ Microbiol

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155

121

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Engineering Pseudomonas putida for improved utilization of syringyl aromatics

Mueller

Willett

Feist

et al. 2022

Biotech & Bioengineering

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Lignin is a largely untapped source for the bioproduction of value-added chemicals.Pseudomonas putida KT2440 has emerged as a strong candidate for bioprocessing of lignin feedstocks due to its resistance to several industrial solvents, broad metabolic capabilities, and genetic amenability. Here we demonstrate the engineering of P. putida for the ability to metabolize syringic acid, one of the major products that comes from the breakdown of the syringyl component of lignin. The rational design was first applied for the construction of strain Sy-1 by overexpressing a native vanillate demethylase. Subsequent adaptive laboratory evolution (ALE) led to the generation of mutations that achieved robust growth on syringic acid as a sole carbon source. The best mutant showed a 30% increase in the growth rate over the original engineered strain. Genomic sequencing revealed multiple mutations repeated in separate evolved replicates. Reverse engineering of mutations identified in agmR, gbdR, fleQ, and the intergenic region of gstB and yadG into the parental strain recaptured the improved growth of the evolved strains to varied extent. These findings thus reveal the ability of P. putida to utilize lignin more fully as a feedstock and make it a more economically viable chassis for chemical production.adaptive lab evolution (ALE), Pseudomonas putida KT2440, syringyl lignin-derived aromatics | INTRODUCTIONLignin is a complex organic polymer that makes up 20%-35% of plant cell walls. It is polymerized from three monolignols: p-coumaryl alcohol, coniferyl alcohol, and sinapyl alcohol, which are categorized as the corresponding hydroxy (H), guaiacyl (G), and syringyl (S) lignin components (Figure 1a;Feofilova & Mysyakina, 2016). Depending on the type of plants, the ratio of H, G, and S subunits in lignin varies, where the grass lignin consists of all subunits, the softwood lignin often has low S component

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A novel cationic β-cyclodextrin decorated with a choline-like pendant exhibits Iodophor, Mucoadhesive and bactericidal properties

Pedotti,

Ferreri,

Migliore

et al. 2024

Carbohydrate Polymers

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Roles of Three Transporters, CbcXWV, BetT1, and BetT3, in Pseudomonas aeruginosa Choline Uptake for Catabolism

Cited by 43 publications

References 48 publications

Homeostasis and Catabolism of Choline and Glycine Betaine: Lessons from Pseudomonas aeruginosa

Homeostasis and Catabolism of Choline and Glycine Betaine: Lessons from Pseudomonas aeruginosa

Engineering Pseudomonas putida for improved utilization of syringyl aromatics

A novel cationic β-cyclodextrin decorated with a choline-like pendant exhibits Iodophor, Mucoadhesive and bactericidal properties

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