Roles of various enzymes in the biotransformation of 6:2 fluorotelomer alcohol (6:2 FTOH) by a white-rot fungus

Merino, Nancy; Wang, Ning; Gao, Yifan; Wang, Meng; Mahendra, Shaily

doi:10.1016/j.jhazmat.2023.131007

Cited by 12 publications

(6 citation statements)

References 39 publications

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“…These observations suggest that both enzymes may have contributed to the biotransformation of 5:3 FTCA or were positively influenced by 5:3 FTCA and its metabolites. The underlying mechanisms warrant further investigation in future studies ( 122 ).…”

Section: Enzymes Involved In Pfas Defluorinationmentioning

confidence: 97%

“…P450s from fungi (such as Cunninghamella elegans and Phanerochaete chrysosporium ) have also been reported to participate in the biotransformation of 6:2 FTOH ( 121 – 123 ). Khan and Murphy ( 121 ) observed that C. elegans failed to transform 6:2 FTOH in the presence of the P450 inhibitor (1-aminobenzotriazole and allylthiourea), supporting a role for P450s in this biotransformation process.…”

Section: Enzymes Involved In Pfas Defluorinationmentioning

confidence: 99%

“…Moreover, they found the inhibition of P450 activity by 5:3 FTCA, the primary metabolite accumulated during the biotransformation of 6:2 FTOH ( 121 ). Merino et al ( 122 ) quantified NADPH-P450 reductase (CPR) activity to indirectly assess the whole P450 catalytic system during the biotransformation of 6:2 FTOH by P. chrysosporium . They noted that CPR activity significantly increased in the presence of 6:2 FTOH compared to its absence, suggesting the engagement of P450s in the biotransformation of 6:2 FTOH.…”

Section: Enzymes Involved In Pfas Defluorinationmentioning

confidence: 99%

“…The genes encoding LiP ( lipD ) and MnP ( mnp1 ) demonstrated differential expression in comparison to the PFAS-free control when P. chrysosporium was cultured under ligninolytic conditions in a modified Kirk medium containing 1% glucose ( 122 ). Specifically, both genes exhibited gradual accumulation of transcripts over time, reaching their peak expression levels on day 28 when P. chrysosporium was cultivated with 5:3 FTCA as the parent compound.…”

Section: Enzymes Involved In Pfas Defluorinationmentioning

confidence: 99%

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Toward the development of a molecular toolkit for the microbial remediation of per-and polyfluoroalkyl substances

Hu,

Scott

2024

Appl Environ Microbiol

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Per- and polyfluoroalkyl substances (PFAS) are highly fluorinated synthetic organic compounds that have been used extensively in various industries owing to their unique properties. The PFAS family encompasses diverse classes, with only a fraction being commercially relevant. These substances are found in the environment, including in water sources, soil, and wildlife, leading to human exposure and fueling concerns about potential human health impacts. Although PFAS degradation is challenging, biodegradation offers a promising, eco-friendly solution. Biodegradation has been effective for a variety of organic contaminants but is yet to be successful for PFAS due to a paucity of identified microbial species capable of transforming these compounds. Recent studies have investigated PFAS biotransformation and fluoride release; however, the number of specific microorganisms and enzymes with demonstrable activity with PFAS remains limited. This review discusses enzymes that could be used in PFAS metabolism, including haloacid dehalogenases, reductive dehalogenases, cytochromes P450, alkane and butane monooxygenases, peroxidases, laccases, desulfonases, and the mechanisms of microbial resistance to intracellular fluoride. Finally, we emphasize the potential of enzyme and microbial engineering to advance PFAS degradation strategies and provide insights for future research in this field.

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Section: Enzymes Involved In Pfas Defluorinationmentioning

confidence: 97%

Section: Enzymes Involved In Pfas Defluorinationmentioning

confidence: 99%

Section: Enzymes Involved In Pfas Defluorinationmentioning

confidence: 99%

Section: Enzymes Involved In Pfas Defluorinationmentioning

confidence: 99%

See 2 more Smart Citations

Toward the development of a molecular toolkit for the microbial remediation of per-and polyfluoroalkyl substances

Hu,

Scott

2024

Appl Environ Microbiol

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“…Additionally, it was shown that this metabolite is inhibitory and that CYP activity is crucial to the degradation of 6:2 FTOH in this fungus. Most recently Merino et al ( 2023 ) demonstrated the involvement of CYPs in the biotransformation of 6:2 FTOH in P. chrysogenum by measuring overall cytochrome P450 reductase activity (CPR) and monitoring CYP gene expression in the presence of 6:2 FTOH and its metabolites. However, the exact enzymes involved in fungal 6:2 FTOH degradation are not known, and only one report of an in vitro biotransformation of 6:2 FTOH to 6:2 FTCA, by a heterologously expressed CYP from C. elegans , has been reported (Khan and Murphy 2022 ).…”

Section: Pesticidesmentioning

confidence: 99%

Recent advances in fungal xenobiotic metabolism: enzymes and applications

Khan,

Hof,

Niemcová

et al. 2023

World J Microbiol Biotechnol

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Fungi have been extensively studied for their capacity to biotransform a wide range of natural and xenobiotic compounds. This versatility is a reflection of the broad substrate specificity of fungal enzymes such as laccases, peroxidases and cytochromes P450, which are involved in these reactions. This review gives an account of recent advances in the understanding of fungal metabolism of drugs and pollutants such as dyes, agrochemicals and per- and poly-fluorinated alkyl substances (PFAS), and describes the key enzymes involved in xenobiotic biotransformation. The potential of fungi and their enzymes in the bioremediation of polluted environments and in the biocatalytic production of important compounds is also discussed.

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Available and emerging liquid treatment technologies for PFASs

DiGuiseppi,

Newell,

Carey

et al. 2024

Remediation Journal

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Per‐ and polyfluoroalkyl substances (PFASs) are present at a wide range of private sector facilities and United States (US) government installations, including those operated by the Department of Defense, the Department of Energy, the National Aeronauts and Space Administration, and other entities, as well as additional sites worldwide. Impacts have been identified in a range of environmental media including drinking water, groundwater, surface water, leachate, wastewater, soil, sediment, and soil gas. Treatment technologies to remove or destroy PFASs cost effectively have proven to be elusive to the industry. However, recent developments are bringing that goal close to reality for some media. This article has been prepared to address only liquid treatment technologies. Soil treatment technologies are presently a lower priority and may be addressed in future articles. The challenge of identifying and evaluating available and emerging liquid treatment technologies was put to the PFAS Experts Symposium in Houston, Texas, on June 7–8, 2023, which was attended by PFAS professionals and subject matter experts with a broad range of backgrounds. The discussions covered a variety of technical approaches and led to the preparation of this manuscript. This article strives to concisely summarize modern technical approaches that are potentially applicable to managing liquid media at PFAS‐impacted sites. Currently, ex situ sorbent technologies using granular activated carbon (GAC) and ion exchange (IX) resin are commercially available and most widely used. Other liquid technologies are summarized and current applications are presented to allow the reader to evaluate each technology for their particular use. This article is not intended to provide guidance on site‐specific design of treatment systems, but instead to serve as an update to earlier articles from this group and others addressing PFAS treatment technologies and related PFAS topics.

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Roles of various enzymes in the biotransformation of 6:2 fluorotelomer alcohol (6:2 FTOH) by a white-rot fungus

Cited by 12 publications

References 39 publications

Toward the development of a molecular toolkit for the microbial remediation of per-and polyfluoroalkyl substances

Toward the development of a molecular toolkit for the microbial remediation of per-and polyfluoroalkyl substances

Recent advances in fungal xenobiotic metabolism: enzymes and applications

Available and emerging liquid treatment technologies for PFASs

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