Rolling the Human Amnion to Engineer Laminated Vascular Tissues

Amensag, Salma; McFetridge, Peter S.

doi:10.1089/ten.tec.2012.0119

Cited by 41 publications

(45 citation statements)

References 40 publications

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“…The amnion is considered to be the load-bearing layer of the FM [9] becoming the focus of mechanical investigations. In addition to its essential physiological function, it was also proposed as a promising candidate to be used as scaffold material for tissue engineering applications [10,11]. The mechanical response of the intact FM and of the separated amnion was investigated in uniaxial tensile tests [7,12,13,14,15,16,17,18], biaxial tensile tests [16,19], puncture tests [9,17,20,21,22,23,24] and inflation tests [3,25,26,27,28,29,30,31].…”

Section: Introductionmentioning

confidence: 99%

Time-dependent mechanical behavior of human amnion: Macroscopic and microscopic characterization

et al. 2015

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Edoardo (2015) Time-dependent mechanical behavior of human amnion: Macroscopic and microscopic characterization. Acta Biomaterialia, 11 . pp. 314-323. ISSN 1878-7568 Access from the University of Nottingham repository: http://eprints.nottingham.ac.uk/45411/1/mauri_actabiomaterialia2014_preprint.pdf Copyright and reuse:The Nottingham ePrints service makes this work by researchers of the University of Nottingham available open access under the following conditions. This article is made available under the Creative Commons Attribution Non-commercial No Derivatives licence and may be reused according to the conditions of the licence. For more details see: http://creativecommons.org/licenses/by-nc-nd/2.5/ A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription. AbstractCharacterizing the mechanical response of the human amnion is essential to understand and to eventually prevent premature rupture of fetal membranes. In this study, a large set of macroscopic and microscopic mechanical tests has been carried out on fresh unfixed amnion to gain insight into the time-dependent material response and the underlying mechanisms. Creep and relaxation responses of amnion were characterized in macroscopic uniaxial tension, biaxial tension and inflation configurations. For the first time, these experiments were complemented by microstructural information from nonlinear laser scanning microscopy performed during in-situ uniaxial relaxation tests. The amnion showed large tension reduction during relaxation and small inelastic strain accumulation in creep. The short-term relaxation response was related to a concomitant in-plane and out-of-plane contraction and was dependent on the testing configuration. The microscopic 2 investigation revealed a large volume reduction at the beginning, but no change of volume was measured long-term during relaxation. Tension-strain curves normalized with respect to the maximum strain were highly repeatable in all configurations and allowed the quantification of corresponding characteristic parameters. The present data indicate that dissipative behavior of human amnion is related to two mechanisms: (i) volume reduction due to water outflow (up to ~20 seconds) and (ii) long-term dissipative behavior without macroscopic deformation and no systematic global reorientation of collagen fibers. IntroductionThe fetal membrane (FM) surrounds the growing fetus and ensures its environment during gestation. Preterm premature rupture of the membrane affects about 3% of all pregnancies and increases the risk of morbidity in the newborn [1]. The etiology of preterm premature rupture of the membrane is complex and not completely understood. Repeated mechanical loading, such as that occurring as a result of fetal movement and labor, was recen...

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Section: Introductionmentioning

confidence: 99%

Time-dependent mechanical behavior of human amnion: Macroscopic and microscopic characterization

et al. 2015

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“…14,15 h AM ECM was cut into 60-mm × 60-mm sheets, rehydrated with 50 mg/mL fibrinogen and rolled around 3.2-mm outer diameter tubing. The resultant six-layered h AM scaffolds were then treated with thrombin to catalyze the formation of fibrin and freeze-dried.…”

Section: Methodsmentioning

confidence: 99%

“…14 Based on previous work that optimized cell seeding density (unpublished), the ablumenal surface of the graft was seeded at a density of 600 cells/mm 2 . 14 Dynamic culture was performed in custom bioreactors connected within a dual circuit perfusion system. Two peristaltic pumps were used to independently control the lumenal and ablumenal flow circuits.…”

Section: Methodsmentioning

confidence: 99%

“…10-13 While a cell-mediated fusion of the layers has proven successful in preliminary studies, this study investigated the use of freeze-drying in conjunction with a fibrin sealant to bind adjacent layers. 6,14 Without the need for pre-population with autologous cells, the conduits would be readily available, easily transportable, and storable extended time periods.…”

Section: Introductionmentioning

confidence: 99%

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Pilot assessment of a human extracellular matrix-based vascular graft in a rabbit model

Amensag

Goldberg

O’Malley

et al. 2017

Journal of Vascular Surgery

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Background Herein we describe a small-diameter vascular graft constructed from rolled human amniotic membrane (hAM), with in vitro evaluation and subsequent in vivo assessment of its mechanical and initial biological viability in the early post-implantation period. This approach for graft construction allows for customization of graft dimensions, with wide-ranging potential clinical applicability as a non-autologous, allogeneic, cell-free graft material. Methods and Results Acellular hAM were rolled into layered conduits (3.2-mm diameter) that were bound with fibrin and lyophilized. In vitro analysis Constructs were seeded with human smooth muscle cells (SMC) and cultured under controlled arterial hemodynamic conditions. SMC were shown to adhere to, proliferate within, and remodel the scaffold over a four-week culture period. At the end of the culture period, there was histologic and biomechanical evidence of graft wall layer coalescence. In vivo analysis The acellular hAM conduits were surgically implanted as arterial interposition grafts into the carotid arteries of immunocompetent rabbits. Grafts demonstrated patency over four weeks (n=3) with no hyperacute rejection or thrombotic occlusion. Explants displayed histologic evidence of active cellular remodeling, with endogenous cell repopulation of the graft wall concurrent with degradation of initial graft material. Cells were shown to align circumferentially to resemble a vascular medial layer. Conclusions The vascular grafts were shown to provide a supportive scaffold allowing for cellular infiltration and remodeling by host cell populations in vivo. Using this approach, “off-the-shelf” vascular grafts can be created with specified diameters and wall thicknesses to satisfy specific anatomical requirements in diverse patient populations.

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“…In additon to uses as a more robust sheet, these modificatons have shown potential in vascular and neuronal applications. [34, 37, 38] With the positive characteristics associated with these materials, it is likely that novel approaches will be taken to modify the base structure and function, either on their own or in combination with other materials, to bring innovative therapies to the clinic.…”

Section: Advantageous Properties For Regenerative Medicinementioning

confidence: 99%

Human Perinatal‐Derived Biomaterials

Moore

Walle

Chang

et al. 2017

Adv Healthcare Materials

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Human perinatal tissues have been used for over a century as allogeneic biomaterials. Due to their advantageous properties including angiogenecity, anti-inflammation, anti-microbial, and immune privilege, these tissues are being utilized for novel applications across wide-ranging medical disciplines. Given continued clinical success, increased adoption of perinatal tissues as a disruptive technology platform has allowed for significant penetration into the multi-billion dollar biologics market. Here, we review current progress and future applications of perinatal biomaterials, as well as associated regulatory issues.

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Rolling the Human Amnion to Engineer Laminated Vascular Tissues

Cited by 41 publications

References 40 publications

Time-dependent mechanical behavior of human amnion: Macroscopic and microscopic characterization

Time-dependent mechanical behavior of human amnion: Macroscopic and microscopic characterization

Pilot assessment of a human extracellular matrix-based vascular graft in a rabbit model

Human Perinatal‐Derived Biomaterials

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