Romanowsky staining: history, recent advances and future prospects from a chemistry perspective

Kalinin, Valeriy; Padnya, Pavel; Stoikov, Ivan

doi:10.1080/10520295.2023.2273860

Cited by 2 publications

(1 citation statement)

References 155 publications

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“…During the necropsy, a sample of the intrathoracic fluid was centrifuged, and the sediment was used for the preparation of cytological smears, and was stained using the Romanowsky technique [ 11 ]. The cytological analysis of the pleural effusion revealed a significant presence of lymphocytes, plasma cells, and macrophages.…”

Section: Case Presentationmentioning

confidence: 99%

Canine Leishmaniosis Associated with Acute Pleural Effusion and Sudden Death in a Dog

Brito,

Sousa,

Sampaio

et al. 2024

Veterinary Sciences

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A two-year-old female crossbreed dog, previously a stray with no known owner, was adopted and subsequently spayed. The dog exhibited weight loss over a period of two months and died suddenly during a leashed walk. Upon necropsy, enlargement of the submandibular, prescapular, and popliteal lymph nodes was noted. The intrathoracic cavity contained a substantial volume of yellowish-white fluid. Lymph nodes in the mediastinal and ventral thoracic centers were also enlarged, hemorrhagic, and friable. Microscopic examination revealed significant architectural changes in the lymph nodes, characterized by a pronounced cellular infiltrate consisting of lymphocytes and histiocytes, along with macrophages containing intracytoplasmic Leishmania amastigotes. Immunohistochemical analysis of the lymph nodes confirmed positive staining for Leishmania amastigotes. This case represents the first report of canine leishmaniasis associated with acute pleural effusion and sudden death.

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Section: Case Presentationmentioning

confidence: 99%