Root Gall Formation, Resting Spore Isolation and High Molecular Weight DNA  Extraction of Plasmodiophora brassicae

The characteristics of 41 native Trichoderma isolates collected from diverse microclimatic domains in Nepal and two Ohio isolates, and their efficacy in biocontrol of plant diseases were assessed. Species assignment was based on a maximum likelihood phylogenetic analysis constructed on a concatenated dataset using the internal transcribed spacer region (ITS), translocation elongation factor (tef1) and RNA polymerase II subunit (rpb2). Most of the Nepal isolates (n=37) were assigned to the Viride clade and identified as T. asperellum or T. asperelloides, while a minority (n=4) were assigned to the Harzianum clade and identified as T. lixii or T. rugulosum. One of the Ohio isolates belonged to the Hamatum clade and was closely related to the T. hamatum reference strain while the other Ohio strain was closely related to T. ghanense (Longibrachiatum clade). Confrontation assays conducted to evaluate mycelial growth reduction of Rhizoctonia solani indicated that mechanisms of action included competition, mycoparasitism, or antibiosis. Trichoderma asperellum isolates NT22, NT8, NT24, and NT25 and T. asperelloides isolates NT1 and NT30 reduced Rhizoctonia root rot severity in greenhouse assays by more than 50% compared with the non- treated controls. Trichoderma asperellum isolates NT4, NT25, NT28, and NT17 and T. asperelloides NT8 suppressed clubroot severity in greenhouse assays by more than 75% compared with the non-treated controls. A significant negative correlation was observed between percent mycelial growth reduction in vitro (confrontation assay) of R. solani and root rot severity. This study provides a basis for additional investigations of Trichoderma diversity and biocontrol potential in Nepal

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Root Gall Formation, Resting Spore Isolation and High Molecular Weight DNA Extraction of Plasmodiophora brassicae

Cited by 2 publications

References 8 publications

Species-specific predation determines the feeding impacts of six soil protist species on bacterial and eukaryotic prey

Species-specific predation determines the feeding impacts of six soil protist species on bacterial and eukaryotic prey

The Pursuit in Nepal of Native Trichoderma spp. for Plant Disease Biocontrol

Contact Info

Product

Resources

About