Root-specific expression of defensin in transgenic tobacco results in enhanced resistance against Phytophthora parasitica var. nicotianae

Lee, Hyun-Hwa; Kim, Jin-Sol; Hoang, Quyen T. N.; Kim, Jeong-Il; Kim, Young Soon

doi:10.1007/s10658-018-1419-6

Cited by 8 publications

(4 citation statements)

References 30 publications

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“…Plant defensins are small cysteine-rich peptides that are active against bacteria and fungi [26,27,28]. The importance of defensins in killing pathogens are well-proven in many plant species, including Arabidopsis [29], Solanum lycopersicum (tomato) [30], and Nicotiana tabacum [31].…”

Section: Different Layers Of Plant Defensementioning

confidence: 99%

Different Pathogen Defense Strategies in Arabidopsis: More than Pathogen Recognition

Zhang

Zhao

Jiang

et al. 2018

Cells

111

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Plants constantly suffer from simultaneous infection by multiple pathogens, which can be divided into biotrophic, hemibiotrophic, and necrotrophic pathogens, according to their lifestyles. Many studies have contributed to improving our knowledge of how plants can defend against pathogens, involving different layers of defense mechanisms. In this sense, the review discusses: (1) the functions of PAMP (pathogen-associated molecular pattern)-triggered immunity (PTI) and effector-triggered immunity (ETI), (2) evidence highlighting the functions of salicylic acid (SA) and jasmonic acid (JA)/ethylene (ET)-mediated signaling pathways downstream of PTI and ETI, and (3) other defense aspects, including many novel small molecules that are involved in defense and phenomena, including systemic acquired resistance (SAR) and priming. In particular, we mainly focus on SA and (JA)/ET-mediated signaling pathways. Interactions among them, including synergistic effects and antagonistic effects, are intensively explored. This might be critical to understanding dynamic disease regulation.

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Section: Different Layers Of Plant Defensementioning

confidence: 99%

Different Pathogen Defense Strategies in Arabidopsis: More than Pathogen Recognition

Zhang

Zhao

Jiang

et al. 2018

Cells

111

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“…Phytophthora nicotianae is one of the most destructive soil-borne oomycetes pathogens in agriculture (Zhang et al, 2018;Lee et al, 2018). The fungus can infect tobacco, citrus, and other plants, causing serious economic losses (Jing et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Effects of manganese and zinc on the growth process of Phytophthora nicotianae and the possible inhibitory mechanisms

Luo

Yao

Tan

et al. 2020

PeerJ

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Background Phytophthora nicotianae is a fungal soil-borne pathogen that damages various plant species. Mancozeb and Zineb, fungicides containing manganese (Mn) and zinc (Zn) as the main components, are widely used to control the diseases caused by Phytophthora. However, the inhibition mechanism is still unclear. The purpose of this study was to examine the effects of Mn and Zn on P. nicotianae and to determine possible inhibitory mechanisms of Mn and Zn on sporangiogenesis of P. nicotianae. Methods The mycelial growth, sporangium generation, zoosporogenesis and zoospore germination of P. nicotianae were observed under Mn and Zn treatments. The gene (csn4 and csn7) expression levels of P. nicotianae in different growth stages were examined. Csn4 and csn7 gene expression, superoxide dismutase (SOD) activity, catalase (CAT) activity and malondialdehyde (MDA) content were tested at the stage of sporangiogenesis under different Mn and Zn concentrations. Results Mycelial growth of P. nicotianae was significantly inhibited by Mn from ≥1 mg/L concentration and by Zn from ≥10 mg/L. The sporangia production, sporangia release, and zoospore germination of P. nicotianae were significantly reduced by Mn at all concentrations, while treatment with Zn from ≥0.5 mg/L concentration significantly inhibited the same processes. At the same concentration, the inhibition rate of Mn on the growth process of P. nicotianae was higher than that of Zn. The csn4 and csn7 gene transcription of P. nicotianae were significantly reduced by all treatments with Mn and Zn at the stage of sporangiogenesis. With the increase of Mn concentration, the activities of SOD and CAT increased to maxima and then decreased, and the content of MDA gradually increased during sporangiogenesis of P. nicotianae. The sporangia production of P. nicotianae was significantly positively correlated with the expression levels of the genes csn4 and csn7. Conclusion The inhibitory effect of Mn on the growth process of P. nicotianae was stronger than that of Zn, especially on sporangiogenesis and zoosporogenesis. A possible mechanism of the inhibitory effect on sporangiogenesis of P. nicotianae was that Mn and Zn acted by inhibiting the expression levels of the genes csn4 and csn7 and by affecting antioxidant enzyme activity (further resulting in lipid peroxidation) in the sporangium of P. nicotianae.

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“…Several genes encoding defensins have been successfully transferred to important plant species such as tobacco (Portieles et al, 2010;Lee et al, 2018), tomato (Abdallah et al, 2010), potato (Gao et al, 2000;Portieles et al, 2010;Kumar and Chakrabarti, 2018), rice (Kanzaki et al, 2002;Jha and Chattoo, 2010), and beans (Espinosa-Huerta et al, 2013), among others, producing resistance to different pathogens. It has been demonstrated that the expression of the NmDef02 defensin gene in tobacco and potato transgenic plants produced a strong resistance against Phytophthora infestans under greenhouse and field conditions (Portieles et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Field Resistance to Phakopsora pachyrhizi and Colletotrichum truncatum of Transgenic Soybean Expressing the NmDef02 Plant Defensin Gene

et al. 2020

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Fungal diseases lead to significant losses in soybean yields and a decline in seed quality; such is the case of the Asian soybean rust and anthracnose caused by Phakopsora pachyrhizi and Colletotrichum truncatum, respectively. Currently, the development of transgenic plants carrying antifungal defensins offers an alternative for plant protection against pathogens. This paper shows the production of transgenic soybean plants expressing the NmDef02 defensin gene using the biolistic delivery system, in an attempt to improve resistance against diseases and reduce the need for chemicals. Transgenic lines were assessed in field conditions under the natural infections of P. pachyrhizi and C. truncatum. The constitutive expression of the NmDef02 gene in transgenic soybean plants was shown to enhance resistance against these important plant pathogens. The quantification of the P. pachyrhizi biomass in infected soybean leaves revealed significant differences between transgenic lines and the non-transgenic control. In certain transgenic lines there was a strong reduction of fungal biomass, revealing a less severe disease. Integration and expression of the transgenes were confirmed by PCR, Southern blot, and qRT-PCR, where the Def1 line showed a higher relative expression of defensin. It was also found that the expression of the NmDef02 defensin gene in plants of the Def1 line did not have a negative effect on the nodulation induced by Bradyrhizobium japonicum. These results indicate that transgenic soybean plants expressing the NmDef02 defensin gene have a substantially enhanced resistance to economically important diseases, providing a sound environmental approach for decreasing yield losses and lowering the burden of chemicals in agriculture.

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Root-specific expression of defensin in transgenic tobacco results in enhanced resistance against Phytophthora parasitica var. nicotianae

Cited by 8 publications

References 30 publications

Different Pathogen Defense Strategies in Arabidopsis: More than Pathogen Recognition

Different Pathogen Defense Strategies in Arabidopsis: More than Pathogen Recognition

Effects of manganese and zinc on the growth process of Phytophthora nicotianae and the possible inhibitory mechanisms

Field Resistance to Phakopsora pachyrhizi and Colletotrichum truncatum of Transgenic Soybean Expressing the NmDef02 Plant Defensin Gene

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