Root water uptake and its pathways across the root: quantification at the cellular scale

Zarebanadkouki, Mohsen; Trtik, Pavel; Hayat, Faisal; Carminati, Andrea; Kaestner, Anders

doi:10.5194/egusphere-egu2020-13520

Cited by 5 publications

(5 citation statements)

References 19 publications

(20 reference statements)

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“…We observed faster movement of AF (using Cel1SP‐GeNL) compared to cytosolic fluid (using GeNL). Similarly, in Lupinus albus root, the velocity of water uptake via the apoplastic pathway is higher than the symplastic pathway (Zarebanadkouki et al, 2019). As Cel1SP‐GeNL bioluminescence could successfully represent the AF flow, this method could be useful for investigating AF dynamics in roots.…”

Section: Discussionmentioning

confidence: 99%

Application of Green‐enhanced Nano‐lantern as a bioluminescent ratiometric indicator for measurement of Arabidopsis thaliana root apoplastic fluid pH

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Osabe

Entani

et al. 2022

Plant Cell & Environment

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Plant root absorbs water and nutrients from the soil, and the root apoplastic fluid (AF) is an important intermediate between cells and the surrounding environment.The acid growth theory suggests that an acidic AF is needed for cell wall expansion during root growth. However, technical limitations have precluded the quantification of root apoplastic fluid pH (AF-pH). Here, we used Green-enhanced Nano-lantern (GeNL), a chimeric protein of the luciferase NanoLuc (Nluc) and the green fluorescent protein mNeonGreen (mNG), as a ratiometric pH indicator based on the pH dependency of bioluminescence resonance energy transfer efficiency from Nluc to mNG. Luminescence spectrum of GeNL changed reciprocally from pH 4.5 to 7.5, with a pK a of 5.5. By fusing GeNL to a novel signal peptide from Arabidopsis thaliana Cellulase 1, we localised GeNL in A. thaliana AF. We visualised AF dynamics at subcellular resolution over 30 min and determined flow velocity in the maturation zone to be 0.97± 0.06 μm/s. We confirmed that the developing root AF is acidic in the pH range of 5.1−5.7, suggesting that the AF-pH is tightly regulated during root elongation. These results support the acid growth theory and provide evidence for AF-pH maintenance despite changes in ambient pH.

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Section: Discussionmentioning

confidence: 99%

Application of Green‐enhanced Nano‐lantern as a bioluminescent ratiometric indicator for measurement of Arabidopsis thaliana root apoplastic fluid pH

Tran

Osabe

Entani

et al. 2022

Plant Cell & Environment

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“…In other approaches, neutron radiography visualization of deuterated water was used to image water uptake sites, which were fed into model-assisted computation approach. These combined analyses were used to infer the corresponding local hydraulic properties and water potential (Zarebanadkouki et al 2016 , 2019 ).…”

Section: Latest Developments and Perspectivesmentioning

confidence: 99%

Experimental and conceptual approaches to root water transport

et al. 2022

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Background Root water transport, which critically contributes to the plant water status and thereby plant productivity, has been the object of extensive experimental and theoretical studies. However, root systems represent an intricate assembly of cells in complex architectures, including many tissues at distinct developmental stages. Our comprehension of where and how molecular actors integrate their function in order to provide the root with its hydraulic properties is therefore still limited. Scope Based on current literature and prospective discussions, this review addresses how root water transport can be experimentally measured, what is known about the underlying molecular actors, and how elementary water transport processes are scaled up in numerical/mathematical models. Conclusions The theoretical framework and experimental procedures on root water transport that are in use today have been established a few decades ago. However, recent years have seen the appearance of new techniques and models with enhanced resolution, down to a portion of root or to the tissue level. These advances pave the way for a better comprehension of the dynamics of water uptake by roots in the soil.

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“…The smallest detectable root diameter depends on the soil moisture (determining the transmission signal and the image contrast between root and soil) and the spatial resolution of the respective measurement. For plant containers with a diameter of 3 cm, the detection limit at moderate soil moisture usually ranges between 0.1 and 0.2 mm (e.g., Mawodza et al., 2020; Zarebanadkouki et al., 2019). Much finer roots (e.g., ≤0.05 mm) can be detected using high‐resolution detector setups.…”

Section: Limitations Of Neutron Imaging Techniquesmentioning

confidence: 99%

Quantification of root water uptake and redistribution using neutron imaging: a review and future directions

et al. 2022

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SUMMARY Quantifying root water uptake is essential to understanding plant water use and responses to different environmental conditions. However, non‐destructive measurement of water transport and related hydraulics in the soil–root system remains a challenge. Neutron imaging, with its high sensitivity to hydrogen, has become an unparalleled tool to visualize and quantify root water uptake in vivo. In combination with isotopes (e.g., deuterated water) and a diffusion–convection model, root water uptake and hydraulic redistribution in root and soil can be quantified. Here, we review recent advances in utilizing neutron imaging to visualize and quantify root water uptake, hydraulic redistribution in roots and soil, and root hydraulic properties of different plant species. Under uniform soil moisture distributions, neutron radiographic studies have shown that water uptake was not uniform along the root and depended on both root type and age. For both tap (e.g., lupine [Lupinus albus L.]) and fibrous (e.g., maize [Zea mays L.]) root systems, water was mainly taken up through lateral roots. In mature maize, the location of water uptake shifted from seminal roots and their laterals to crown/nodal roots and their laterals. Under non‐uniform soil moisture distributions, part of the water taken up during the daytime maintained the growth of crown/nodal roots in the upper, drier soil layers. Ultra‐fast neutron tomography provides new insights into 3D water movement in soil and roots. We discuss the limitations of using neutron imaging and propose future directions to utilize neutron imaging to advance our understanding of root water uptake and soil–root interactions.

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Root water uptake and its pathways across the root: quantification at the cellular scale

Cited by 5 publications

References 19 publications

Application of Green‐enhanced Nano‐lantern as a bioluminescent ratiometric indicator for measurement of Arabidopsis thaliana root apoplastic fluid pH

Application of Green‐enhanced Nano‐lantern as a bioluminescent ratiometric indicator for measurement of Arabidopsis thaliana root apoplastic fluid pH

Experimental and conceptual approaches to root water transport

Quantification of root water uptake and redistribution using neutron imaging: a review and future directions

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