Rooting of carnation cuttings

Acosta, Manuel; Oliveros-Valenzuela, M. Rocío; Nicolás, Carlos; Sánchez-Bravo, José

doi:10.4161/psb.4.3.7912

Cited by 10 publications

(9 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Adventitious rooting is affected by multiple endogenous factors; one of the endogenous factors known to play an important role in the adventitious root formation is auxin (Acosta et al 2009;Liu et al 2013;Sukumar et al 2013). Interestingly, in agreement with a study in the Chrysanthemum, there is auxin-induced protein expressed in differentiated root primordial (5 days).…”

Section: Hormone-related and Oxidation Reaction Proteinssupporting

confidence: 84%

Comparative proteomic analysis of tetraploid black locust (Robinia pseudoacacia L.) cuttings in different phases of adventitious root development

Sheng

Zhao

Zhang

et al. 2014

Trees

View full text Add to dashboard Cite

Key message Comparative proteome analysis revealed the proteins that are differentially expressed during the processes of tetraploid black locust (Robinia pseudoacacia L.) adventitious root formation, which are involved in carbohydrate binding, energy and metabolism, and stress response. Abstract The tetraploid black locust (Robinia pseudoacacia L.) is an important fast-growing tree used in afforestation and is also a protein-rich feed for poultry. Currently, the primary approach for tetraploid black locust reproduction is through cutting propagation. To identify the specific proteins in the rooting process, including the formation of adventitious root primordium (ARP) and the elongation of adventitious root (EAR), a comparative proteomic analysis of the ARP and EAR of tetraploid black locust was performed to identify related proteins that may be involved in regulating ARP formation and the process of EAR. High-resolution two-dimensional electrophoresis (2-DE) followed by colloidal Coomassie staining and mass spectrometric (MS) analysis was used to identify differentially expressed proteins in ARP and EAR. A total of 84 protein spots showed significant expression changes by 2-DE and were successfully identified by MALDI TOF/ TOF MS/MS. Of these, 29 proteins were differentially expressed during the ARP phase, and the remaining 55 proteins did so in the EAR phase. The majority of identified proteins were classified into functional categories, including carbohydrate binding, energy and metabolism, protein degradation/folding and import, cellular cytoskeleton, hormonerelated and oxidation reactions, stress response, cell proliferation, and transcription regulation. Quantitative real-time PCR analysis was performed for selected proteins and showed that not all of the protein expression levels were consistent with the mRNA levels. This study provides the basis for further functional studies of differentially expressed proteins, which will contribute to the understanding of the biochemical processes in adventitious root primordium formation and the elongation of adventitious root. Abbreviations 2-DE Two-dimensional gel electrophoresis MALDI Matrix assisted laser desorption ionization TOF Time of flight TOF MS Time of flight mass spectrometry MS Mass spectrometry IntroductionTetraploid black locust (Robinia pseudoacacia L.) (TRP), which can be produced by doubling the chromosomes of a diploid black locust (2n 9 2) artificially, is a leguminous Communicated by R. Hampp. Electronic supplementary materialThe online version of this article (

show abstract

Section: Hormone-related and Oxidation Reaction Proteinssupporting

confidence: 84%

Comparative proteomic analysis of tetraploid black locust (Robinia pseudoacacia L.) cuttings in different phases of adventitious root development

Sheng

Zhao

Zhang

et al. 2014

Trees

View full text Add to dashboard Cite

show abstract

“…Using inhibitors of polar auxin transport, various investigations in cuttings or de-rooted seedlings have provided evidence for a significant contribution of this type of transport to AR (e.g., Nordström and Eliasson, 1991; Liu and Reid, 1992; Koukourikou-Petridou and Bangerth, 1997; Guerrero et al, 1999; Garrido et al, 2002; Nicolás et al, 2004). Few studies analyzing the expression of genes encoding auxin carriers during adventitious rhizogenesis were conducted in de-rooted pine seedlings (Brinker et al, 2004), intact rice plants (Xu et al, 2005), carnation cuttings (Oliveros-Valenzuela et al, 2008; Acosta et al, 2009), and mango cotyledon segments (Li et al, 2012). The studies with carnation and mango showed the requirement of increased expression of auxin transporters and increase of polar auxin transport during the induction and formation phase of AR.…”

Section: The Central Role Of Auxins: Transport Control Of Local Concmentioning

confidence: 99%

“…Considering cuttings used for vegetative propagation, the progressive accumulation and local concentration of auxin in the base of the cuttings seems to be important to generate the peak necessary for starting the rooting process (Acosta et al, 2009) and often this can be facilitated by exogenous application of auxins in recalcitrant species. Meanwhile, recent studies indicate that basipetal auxin transport and auxin accumulation in the rooting zone may be negatively regulated by strigolactones (Rasmussen et al, 2012).…”

Section: The Central Role Of Auxins: Transport Control Of Local Concmentioning

confidence: 99%

When stress and development go hand in hand: main hormonal controls of adventitious rooting in cuttings

Costa¹,

Almeida²,

Ruedell³

et al. 2013

Front. Plant Sci.

311

280

View full text Add to dashboard Cite

Adventitious rooting (AR) is a multifactorial response leading to new roots at the base of stem cuttings, and the establishment of a complete and autonomous plant. AR has two main phases: (a) induction, with a requirement for higher auxin concentration; (b) formation, inhibited by high auxin and in which anatomical changes take place. The first stages of this process in severed organs necessarily include wounding and water stress responses which may trigger hormonal changes that contribute to reprogram target cells that are competent to respond to rooting stimuli. At severance, the roles of jasmonate and abscisic acid are critical for wound response and perhaps sink strength establishment, although their negative roles on the cell cycle may inhibit root induction. Strigolactones may also inhibit AR. A reduced concentration of cytokinins in cuttings results from the separation of the root system, whose tips are a relevant source of these root induction inhibitors. The combined increased accumulation of basipetally transported auxins from the shoot apex at the cutting base is often sufficient for AR in easy-to-root species. The role of peroxidases and phenolic compounds in auxin catabolism may be critical at these early stages right after wounding. The events leading to AR strongly depend on mother plant nutritional status, both in terms of minerals and carbohydrates, as well as on sink establishment at cutting bases. Auxins play a central role in AR. Auxin transporters control auxin canalization to target cells. There, auxins act primarily through selective proteolysis and cell wall loosening, via their receptor proteins TIR1 (transport inhibitor response 1) and ABP1 (Auxin-Binding Protein 1). A complex microRNA circuitry is involved in the control of auxin response factors essential for gene expression in AR. After root establishment, new hormonal controls take place, with auxins being required at lower concentrations for root meristem maintenance and cytokinins needed for root tissue differentiation.

show abstract

“…It is found that for the cuttings made of stem fragments located at the top of the shoots, the rooting percentage was over 97%, followed by the medium level with 93%, while the cuttings made from the base of the shoots were rooted in proportion of 81%. These differences are explained by the theories regarding the presence and circulation of hormones in plants and those related to the apical dominance, being known that auxin, responsible for the formation of adventitious roots, is found in the highest concentration in the apical part of the plant, from where it has a downward path [ACOSTA & al. 2009].…”

Section: Resultsmentioning

confidence: 99%

“…(2010) studied the role of nitric oxide in the process of AR formation in Chrysanthemum. The progressive accumulation and local concentration of auxin at the base of the cuttings seems to be important for initiating the rooting process [ACOSTA & al. 2009].…”

Section: Introductionmentioning

confidence: 99%

DEVELOPMENT AND TESTING A NEW TECHNOLOGY FOR PRODUCTION OF CHRYSANTHEMUMS PLANTING MATERIAL (CHRYSANTHEMUM s.l.)

Cojocariu¹,

Tănase²

2019

J. Plant Develop.

View full text Add to dashboard Cite

Chrysanthemums represent an important horticultural segment with uses in the autumnal to winter season, aesthetically extending the capitalization of urban open spaces. Propagation by cuttings at Chrysanthemum is the most convenient method of vegetative multiplication. There is, however, a great variability in the ability to form adventitious roots and regenerate new individuals by cuttings. Some cultivars are relatively easy to multiply by cuttings, and other species have a low capacity to rooting. In the present paper we focused on researches regarding the propagation of chrysanthemums (a1-Chrysathemum indicum, a2-Chrysanthemum × grandiflorum) by new type of cuttings (stem fragment with one leaf and axillary bud-SFLAB, b2) and on the evaluation of the capacity of rooting, given the increasing demand for floral planting material as a result of the development of landscape design interest in public spaces but especially private one. The main objective was to establish the influence of the cuttings characteristics (type of cuttings, with two variants-b1-control, top stem fragment-TSF; and b2, stem fragment with one leaf and axillary bud-SFLAB) on the rooting process and the length of developed roots in four variants of rooting substrate (c1-c4) for both types of proposed Chrysanthemum cuttings (b1-b2).

show abstract

Rooting of carnation cuttings

Cited by 10 publications

References 20 publications

Comparative proteomic analysis of tetraploid black locust (Robinia pseudoacacia L.) cuttings in different phases of adventitious root development

Comparative proteomic analysis of tetraploid black locust (Robinia pseudoacacia L.) cuttings in different phases of adventitious root development

When stress and development go hand in hand: main hormonal controls of adventitious rooting in cuttings

DEVELOPMENT AND TESTING A NEW TECHNOLOGY FOR PRODUCTION OF CHRYSANTHEMUMS PLANTING MATERIAL (CHRYSANTHEMUM s.l.)

Contact Info

Product

Resources

About