Background and Objectives
β‐amylase plays a vital role in starch hydrolysis during mashing to obtain fermentable wort for beer production. Since starch gelatinization requires a high temperature, the catalytic activity, and thermostability of β‐amylase are crucial for obtaining high‐quality malt owing to its heat‐labile character. This study aimed to determine the variation in thermostability of β‐amylase among a diverse set of barley genotypes (hulled, hulless, mutants, and wild).
Findings
Statistical analysis indicated the significant effect of genotype and temperature on β‐amylase activity. The mutant genotypes had a wider variation for β‐amylase activity followed by hulled, hulless, and wild genotypes. The % relative residual activity was found to be the highest at 55°C for all the genotypes. Among 54 barley genotypes, PL891 (hulless) was reported to have the highest activity at all temperatures followed by BL1714 (hulled) and BL2105 (mutant) which shows the thermostable nature of β‐amylase in these genotypes. The β‐amylase activity was positively correlated with the amount of reducing sugars formed at a 1% level of significance (r = .852), further corroborating the results.
Conclusions
The β‐amylase activity declined after 55°C in the majority of the genotypes depicting its optimum temperature. Few genotypes, namely PL891, BL1714, and BL2105 had been identified that possessed high β‐amylase activity at all temperatures.
Significance and Novelty
β‐amylase with high thermostability may prove more helpful than selecting the genotype with higher β‐amylase levels if one attempts to increase the amount of fermentable sugars in the wort. Therefore, in the current study genotypes identified with high thermostability of β‐amylase may be exploited in the genetic improvement programs for incorporating beneficial traits into low malting efficiency cultivars but possessing high yield and resistance to various stresses.