Roots of the xerophyte Panicum turgidum host a cohort of ionizing-radiation-resistant biotechnologically-valuable bacteria

Guesmi, Sihem; Najjari, Afef; Pujic, Petar; Ghedira, Kaïs; Ouertani, Rania; Jabberi, Marwa; Chérif, Ameur; Normand, Philippe; Sghaier, Haïtham

doi:10.1016/j.sjbs.2021.09.020

Cited by 7 publications

(7 citation statements)

References 62 publications

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“…Our findings revealed that the predominant phyla in the studied communities are Proteobacteria and Actinobacteria. These results are consistent with the majority of the reports on the microbiota of the Arabian Peninsula desert ( Inostroza et al., 2017 ; Bang et al., 2018 ; Andrés-Barrao et al., 2017 ; Eida et al., 2018 ; Guesmi et al., 2022 ; Li et al., 2023 ; Osman et al., 2016 ). Bacteria of these phyla serve crucial ecological roles to their host plants.…”

Section: Discussionsupporting

confidence: 93%

“…Among the four studied plants, H. persicum (HP) , and H. strobilaceum (HS) have the most enriched microbial communities, followed by P. turgidum (PT), whereas A. hispidissima (AH) exhibited the least diverse microbial composition. To the best of our knowledge, our study is the first report on the AH microbiome while there are some previous reports on the microbiome of PT, HP, and HS, with a focus on rhizosphere microbes ( Guesmi et al., 2022 ; Li et al., 2023 ).…”

Section: Discussionmentioning

confidence: 96%

“…Our data reveals that the rhizosphere of P. turgidum (PT) is enriched in radiotolerant, halotolerant, and thermotolerant bacteria. This aligns with a previous study, which cultured various radiation-resistant species from PT, such as Promicromonospora panici , Kocuria rhizophila , Micrococcus sp., and Microbacterium sp ( Guesmi et al., 2022 ).. In the same study, researchers conducted metagenomic analysis to examine the bacterial community of irradiated roots.…”

Section: Discussionmentioning

confidence: 99%

“…The results demonstrated a shift in microbial structure characterized by an increased abundance of Actinobacteria and Proteobacteria. Interestingly, this shift was accompanied by the induction of metabolic pathways related to oxidative stress tolerance, including DNA repair ( Guesmi et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

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High-throughput sequencing reveals the structure and metabolic resilience of desert microbiome confronting climate change

Mousa,

Abu-Izneid,

Salah-Tantawy

2024

Front. Plant Sci.

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IntroductionDesert ecosystems harbor a unique microbial diversity that is crucial for ecological stability and biogeochemical cycles. An in-depth understanding of the biodiversity, compositions, and functions of these microbial communities is imperative to navigate global changes and confront potential threats and opportunities applicable to agricultural ecosystems amid climate change. MethodsThis study explores microbial communities in the rhizosphere and endosphere of desert plants native to the Arabian Peninsula using next-generation sequencing of the 16S rRNA gene (V3-V4 hypervariable region). ResultsOur results reveal that each microbial community has a diverse and unique microbial composition. Based on alpha and beta diversity indices, the rhizosphere microbiome is significantly diverse and richer in microbial taxa compared to the endosphere. The data reveals a shift towards fast-growing microbes with active metabolism, involvement in nutrient cycling, nitrogen fixation, and defense pathways. Our data reveals the presence of habitat-specific microbial communities in the desert, highlighting their remarkable resilience and adaptability to extreme environmental conditions. Notably, we observed the existence of radiation-resistant microbes such as Deinococcus radiotolerans, Kocuria sp., and Rubrobacter radiotolerans which can tolerate high levels of ionizing radiation. Additionally, examples of microbes exhibiting tolerance to challenging conditions include Nocardioides halotolerans, thriving in high-salinity environments, and hyperthermophilic microbes such as Quasibacillus thermotolerans. Moreover, functional analysis reveals enrichment in chaperon biosynthesis pathways associated with correct protein folding under heat stress conditions.DiscussionOur research sheds light on the unique diversity of desert microbes and underscores their potential applications to increase the resilience of agriculture ecosystems, offering a promising strategy to fortify crops against the challenges posed by climate change, ultimately supporting sustainable food production for our ever-expanding global population.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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High-throughput sequencing reveals the structure and metabolic resilience of desert microbiome confronting climate change

Mousa,

Abu-Izneid,

Salah-Tantawy

2024

Front. Plant Sci.

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“…Kocuria rhizophila emerged as a statistically significant strain, with its incidence showing a significant increase in patients post-radiotherapy( 52,53 ). Similarly, although not statistically significant, similar observations were noted for strains of Micrococcus luteus .…”

Section: Discussionmentioning

confidence: 99%

Urine microbiome changes during and after radiotherapy for prostate cancer

Złoch,

Sibińska,

Monedeiro

et al. 2024

Preprint

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Background: The urinary microbiome may play a new important role in the development of complications, but still, there is no information about their changes during and after radiotherapy (RT). This study aimed to use the matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) technique to identify the microbiome and assess its changes in urine samples of 88 patients irradiated for prostate cancer. Material and methods: Blood for biochemical analysis and urine samples for MALDI were collected at various time points before gold fiducial implantation (t1) at the beginning (t2) and end of radiotherapy (t3); during follow-up, 1 (t4), 4 (t5), 7 (t6) months after the end of treatment. Results: We identified 1801 different microbial isolates, in 89% (470/528) samples revealed the presence of at least one microbial species among which 79% (373/470) were polymicrobial. Species level: 136 G+, 29 G-, 2 Candida have been noted. The far most abundant group of the identified microorganisms was Staphylococcus members - 51.6% of all isolates followed by Micrococcus (9.1%), Enterococcus (7.6%), Kocuria (5.6%), Corynebacterium (5.4%), and Streptococcus (2.2%). A lower variety of microorganisms incident was observed at the end of RT. The total number of species (TNS) was 50 at t1, increased up to 61 at t2, and then fell to the initial value of 52 at t3. The increase in biodiversity was noted after radiotherapy t4-68, t5-86, and t6-75 (p<0.05). Changes in the biodiversity of the urinary microbiota were also reflected in the differences in the total number of isolates (TNI) - 261, 281, and 273 for time points t1-t3 compared to the 292, 362, and 332 for time points t4-t6 as well as in the total number of detected genera (TNG) - 25, 29, 23 (t1-t3) and 28, 38, 31 (t4-t6). Actinomyces, Corynebacterium, Staphylococcus, Streptococcus, demonstrated significant correlation with the RT stages. Concerning individual species, only K. rhizophila abundance significantly increased with time (p=0.045). Bacteria incidence was strongly correlated with glucose levels in urine. The same correlation was observed for glucose levels in blood, but in a weak manner. Staphylococcus presence was related to higher tPSA. Conclusion: RT for prostate cancer induces a dynamic response in the urinary microbiome, characterized by an initial reduction in diversity post-RT followed by a subsequent increase. Our findings highlight the significant influence of glucose levels in both urine and blood on the urinary microbiota. These insights contribute to the evolving understanding of the interplay between RT, the urinary microbiome, and patient health, paving the way for more targeted interventions and personalized approaches in prostate cancer treatment.

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Biotechnological potential of Kocuria rhizophila PT10 isolated from roots of Panicum turgidum

Guesmi

Mahjoubi

Pujic

et al. 2022

Int. J. Environ. Sci. Technol.

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Roots of the xerophyte Panicum turgidum host a cohort of ionizing-radiation-resistant biotechnologically-valuable bacteria

Cited by 7 publications

References 62 publications

High-throughput sequencing reveals the structure and metabolic resilience of desert microbiome confronting climate change

High-throughput sequencing reveals the structure and metabolic resilience of desert microbiome confronting climate change

Urine microbiome changes during and after radiotherapy for prostate cancer

Biotechnological potential of Kocuria rhizophila PT10 isolated from roots of Panicum turgidum

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