Rootstock-induced physiological and biochemical mechanisms of drought tolerance in sweet orange

Gonçalves, Luana Pereira; Alves, Tainã F. O.; Martins, Cristina P. S.; Sousa, Aurizângela Oliveira de; Santos, Ivanildes Conceição dos; Pirovani, Carlos Priminho; Almeida, Alex-Alan Furtado de; Filho, M. A. Coelho; Gesteira, Abelmon S.; Filho, Walter dos Santos Soares; Girardi, Eduardo Augusto; Costa, Márcio Gilberto Cardoso

doi:10.1007/s11738-016-2198-3

Cited by 34 publications

(28 citation statements)

References 45 publications

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“…limonia Osbeck) were used in the drought stress experiment. The experiment was carried out under greenhouse conditions, with the plants grown in plastic pots of 45L containing a mixture of Oxisol and washed sand (3:1 ratio) and subjected to control (leaf predawn water potential between -0.2 to -0.4 MPa) or drought stress (leaf predawn water potential of -1.5 MPa) treatments, as previously described [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

“…The FW and root length of individual seedlings was measured 30 days after the treatments. At the end of the experiment, lipid peroxidation was determined by measuring the thiobarbituric acid reactive substance (TBARS) content according to the method described by [ 37 ]. For the soil dry-down experiment, 30-day-old WT and transgenic (T 1 generation) plants, with an average of 10 to 15 leaves, were first transplanted into 20-liter pots.…”

Section: Methodsmentioning

confidence: 99%

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Genome-wide characterization and expression analysis of citrus NUCLEAR FACTOR-Y (NF-Y) transcription factors identified a novel NF-YA gene involved in drought-stress response and tolerance

et al. 2018

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Nuclear factor Y (NF-Y) is a ubiquitous transcription factor found in eukaryotes. It is composed of three distinct subunits called NF-YA, NF-YB and NF-YC. NF-Ys have been identified as key regulators of multiple pathways in the control of development and tolerance to biotic and abiotic factors. The present study aimed to identify and characterize the complete repertoire of genes coding for NF-Y in citrus, as well as to perform the functional characterization of one of its members, namely CsNFYA5, in transgenic tobacco plants. A total of 22 genes coding for NF-Y were identified in the genomes of sweet orange (Citrus sinensis) and Clementine mandarin (C. clementina), including six CsNF-YAs, 11 CsNF-YBs and five CsNF-YCs. Phylogenetic analyses showed that there is a NF-Y orthologous in the Clementine genome for each sweet orange NF-Y gene; this was not observed when compared to Arabidopsis thaliana. CsNF-Y proteins shared the same conserved domains with their orthologous proteins in other organisms, including mouse. Analysis of gene expression by RNA-seq and EST data demonstrated that CsNF-Ys have a tissue-specific and stress inducible expression profile. qRT-PCR analysis revealed that CsNF-YA5 exhibits differential expression in response to water deficit in leaves and roots of citrus plants. Overexpression of CsNF-YA5 in transgenic tobacco plants contributed to the reduction of H2O2 production under dehydration conditions and increased plant growth and photosynthetic rate under normal conditions and drought stress. These biochemical and physiological responses to drought stress promoted by CsNF-YA5 may confer a productivity advantage in environments with frequent short-term soil water deficit.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Genome-wide characterization and expression analysis of citrus NUCLEAR FACTOR-Y (NF-Y) transcription factors identified a novel NF-YA gene involved in drought-stress response and tolerance

et al. 2018

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“…Briefly, samples were ground in liquid nitrogen in the presence of 0.7% (w/w)polyvinylpyrrolidone (PVP) and subsequently homogenized in the appropriate extraction buffer 16 . After sonication (8 pulses of 5 s each, with intervals of 10 s) and centrifugation (13,000 g , 10 min, 4 °C), the supernatant was used to determine the SOD, CAT, GPX and APX activities as described in Gonçalves et al .…”

Section: Methodsmentioning

confidence: 99%

“…After sonication (8 pulses of 5 s each, with intervals of 10 s) and centrifugation (13,000 g , 10 min, 4 °C), the supernatant was used to determine the SOD, CAT, GPX and APX activities as described in Gonçalves et al . 16 .…”

Section: Methodsmentioning

confidence: 99%

“…However, despite its agricultural importance, whether and how the memory to stress protects citrus plants during successive stress events remains to be explored. Water deficit has been shown to trigger a wide range of responses in citrus, including physiological, biochemical, hormonal and gene expression changes that improve the survival of these plants under water-limiting conditions 15 , 16 . Citrus rootstock combinations have been identified as a promising strategy to improve the fitness of this crop when challenged by moderate or severe drought 15 , 17 , 18 .…”

Section: Introductionmentioning

confidence: 99%

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Recurrent water deficit causes epigenetic and hormonal changes in citrus plants

Neves

Almeida²,

Santana-Vieira

et al. 2017

Sci Rep

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The present study evaluated the physiological, molecular and hormonal parameters from scion/rootstock interaction of citrus plants during recurrent water deficit. Responses of the Valencia (VO) scion variety grafted on two rootstocks with different soil water extraction capacities, Rangpur Lime (RL) and Sunki Maravilha (SM), during three successive periods of water deficit: plants exposed to a single episode of water deficit (WD1) and plants exposed to two (WD2) and three (WD3) recurrent periods of WD were compared. The combinations VO/RL and VO/SM presented polymorphic alterations of epigenetic marks and hormonal (i.e. abscisic acid, auxins and salicylicacid) profiles, which were particularly prominent when VO/SM plantswere exposed toWD3 treatment. Upon successive drought events, the VO/SM combination presented acclimatization characteristics that enable higher tolerance to water deficit by increasing transpiration (E), stomatal conductance (g s) and photosynthetic rate (A), which in turn may have facilitated the whole plant survival. Besides providing comprehensive data on the scion/rootstock interactions upon successive stress events, this study brings the first dataset suggesting that epigenetic alterations in citrus plants triggered by recurrent water deficit lead to improved drought tolerance in this crop species.

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Abiotic Stress Resistance

Piero

Roberta²

2020

Compendium of Plant Genomes

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Rootstock-induced physiological and biochemical mechanisms of drought tolerance in sweet orange

Cited by 34 publications

References 45 publications

Genome-wide characterization and expression analysis of citrus NUCLEAR FACTOR-Y (NF-Y) transcription factors identified a novel NF-YA gene involved in drought-stress response and tolerance

Genome-wide characterization and expression analysis of citrus NUCLEAR FACTOR-Y (NF-Y) transcription factors identified a novel NF-YA gene involved in drought-stress response and tolerance

Recurrent water deficit causes epigenetic and hormonal changes in citrus plants

Abiotic Stress Resistance

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