ROS-scavenging-associated transcriptional and biochemical shifts during nectarine fruit development and ripening

Vall‐llaura, Núria; Fernández-Cancelo, Pablo; Nativitas-Lima, Isabel; Echeverría, Gemma; Teixidó, Neus; Larrigaudière, Christian; Giné-Bordonaba, Jordi

doi:10.1016/j.plaphy.2021.12.022

Cited by 23 publications

(12 citation statements)

References 64 publications

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“…Reactive oxygen species (ROS), as a common product of plants exposed to stress, not only cause irreversible damage to cells but also attack macromolecules [ 48 ]. Excess ROS accumulation promotes pulp decomposition and reduces fruit quality [ 49 ]. Under normal circumstances, the antioxidant system maintains ROS production and metabolism in a dynamic balance.…”

Section: Discussionmentioning

confidence: 99%

“…The higher SOD activity in the FPR (CK) group than in the other groups enhanced the fruit’s ability to remove O 2 − . The enzymatic reaction of SOD led to rapid accumulation of H 2 O 2 , which in turn promoted the scavenging action of POD and CAT [ 49 , 50 ]. Compared with that in the FPR (CK) group, the POD activity in the FPB and FBR treatment groups was markedly decreased.…”

Section: Discussionmentioning

confidence: 99%

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Integrated Physiological and Metabolomic Analyses Reveal the Differences in the Fruit Quality of the Blueberry Cultivated in Three Soilless Substrates

Yang

Duan

Wei

et al. 2022

Foods

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With improving living standards, traditional blueberry planting modes cannot meet commercial demands, and blueberry cultivation with soilless substrate has become a popular solution in the blueberry industry. In this study, different soilless substrate treatments were found to markedly influence fruit appearance and intrinsic quality. The fruit in the 50:50 peat/pine bark (v/v) (FPB) treatment group had the maximum single fruit weight, largest vertical diameter, and brightest color, as well as the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) value, solid-acid ratio and anthocyanin content. The fruit in the 50:50 pine bark/rice husk (v/v) (FBR) treatment group had the highest total phenol and flavonoid levels, largest drip loss value, and lowest total pectin content and firmness value. Metabolomic analysis showed that flavonoid, carbohydrate, and carbohydrate conjugate, and amino acid, peptide, and analog levels were significantly different between groups. Fruit in the FPB group had the highest sucrose, D-fructose 1,6-bisphosphate, salidroside, tectorigenin, naringenin chalcone, trifolirhizin, and galangin contents. The increase in the relative expression of phenylalanine (Phe) promoted the synthesis of fruit polyphenols in the FBR group. Our results provide new insights into the effects of different substrates on the quality of blueberries and a reference for the soilless substrate cultivation of blueberries.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Integrated Physiological and Metabolomic Analyses Reveal the Differences in the Fruit Quality of the Blueberry Cultivated in Three Soilless Substrates

Yang

Duan

Wei

et al. 2022

Foods

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“…In a previous study, Pilati et al [ 33 ] observed a rapid accumulation of H 2 O 2 during grape ripening, which may be associated with grape softening and ripening. In addition, the H 2 O 2 levels linearly increased as the nectarine fruit developed and ripened on trees [ 32 ]. The promoting effect of H 2 O 2 on fruit ripening has also been observed in pears [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

“…During peach fruit ripening, O 2 •− is required at the middle stage of fruit development, and H 2 O 2 acts as a potential signaling molecule to stimulate the late stage of fruit development [ 31 ]. In addition, H 2 O 2 is a signaling molecule that accumulates during the ripening of nectarine fruit and at the beginning of grape berry ripening [ 32 , 33 ]. Oxidative stress is also an integrative factor for triggering tomato fruit ripening, which suggests the signaling role of oxidants in initiating fruit ripening [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

A D-cysteine desulfhydrase, SlDCD2, participates in tomato fruit ripening by modulating ROS homoeostasis and ethylene biosynthesis

Zhao

Yao

et al. 2023

Horticulture Research

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Hydrogen sulfide (H2S) is involved in multiple processes during plant growth and development. D-cysteine desulfhydrase (DCD) can produce H2S with D-cysteine as the substrate; however, the potential developmental roles of DCD have not been explored during the tomato lifecycle. In the present study, SlDCD2 showed increasing expression during fruit ripening. Compared with the control fruits, the silencing of SlDCD2 by pTRV2-SlDCD2 accelerated fruit ripening. A SlDCD2 gene-edited mutant was constructed by CRISPR/Cas9 transformation, and the mutant exhibited accelerated fruit ripening, decreased H2S release, higher cysteine and ethylene contents, enhanced chlorophyll degradation and increased carotenoid accumulation. Additionally, the expression of multiple ripening-related genes, including NYC1, PAO, SGR1, PDS, PSY1, ACO1, ACS2, E4, CEL2 and EXP, was enhanced during the dcd2 mutant tomato fruit ripening. Compared with the wild-type fruits, SlDCD2 mutation induced H2O2 and malondialdehyde (MDA) accumulation in fruits, which led to an imbalance in reactive oxygen species (ROS) metabolism. A correlation analysis indicated that H2O2 was strongly positively correlated with carotenoid and ripening-related gene expression and negatively correlated with the chlorophyll and ethylene contents. Additionally, the dcd2 mutant showed earlier leaf senescence, which may be due to disturbed ROS homeostasis. In short, our findings show that SlDCD2 is involved in H2S generation and that the reduction in endogenous H2S production in the dcd2 mutant causes accelerated fruit ripening and premature leaf senescence. Additionally, decreased H2S in the dcd2 mutant causes excessive H2O2 accumulation and increased ethylene release, suggesting a role of H2S and SlDCD2 in modulating ROS homeostasis and ethylene biosynthesis.

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“…Plant develops several ROS-scavenging mechanism to counter adverse impacts of ROS accumlation and to establish appropriate ROS homeostasis. Antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX) and catalase (CAT) plays a vital role in ROS scavenging and maintain homestasis in fruit and plants ( Apel and Hirt, 2004 ; Vall-Llaura et al., 2022 ). ROS apart from being considered a toxic by-product, also plays significant role as a signaling molecule in various biological processes including fruit development, ripening and responses to various abiotic and biotic stresses ( Fichman and Mittler, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Fruit nutritional composition, antioxidant and biochemical profiling of diverse tomato (Solanum lycopersicum L.) genetic resource

2022

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Tomato is the second most important vegetable crop consumed globally, by the virtue of its antioxidant-rich phytochemicals and bioactive compounds. Identifying genotypes with high antioxidant capacities and nutritionally rich phytochemicals is imperative for improving human health. The present study aimed to analyze 21 antioxidant and nutritional compounds in 93 geographically diverse, high yielding, better quality, stress tolerant tomato genotypes (hybrids, parental lines, inbred lines, and advanced lines). Significant variation (p < 0.05) was detected for investigated traits among the tested genotypes. Principal component analysis revealed the hybrids NIAB-Jauhar, Iron-lady F1, NBH-258, Ahmar F1, NIAB-Gohar, the parents H-24, B-25, AVTO1080, Astra and AVTO1003, as well as the lines LBR-17, AVTO1315, AVTO1311 and Lyp-1 revealed superior performance for the traits such as chlorophylls, lycopene, total carotenoids, total antioxidant capacity, total oxidant status, protease, alpha-amylase and total flavonoid content. Whereas the hybrids Surkhail F1, NBH-204, NBH-229, NBH-151, NBH-196, NBH-152, NBH-261, NBH-228, NIAB-Jauhar, NBH-256 and NBH-255, the lines 21354, AVTO1315, Newcherry, LA4097, AVTO1311 and UAF-1 together with the parents Naqeeb, NCEBR-5, M-82 and LBR-10 exhibited significant contribution to the traits such as total soluble sugars, reducing sugars, malondialdehyde, ascorbic acid, esterase, peroxidase and superoxide dismutase. Moreover, the semi-determinate and determinate tomato genotypes together with the categories parent and line with positive factor scores of 3.184, 0.015, 0.325 and 0.186 in PC- I, exhibited better performance for the trait such as total chlorophylls, lycopene, total carotenoids, total oxidant status, protease, alpha-amylase, total antioxidant capacity, esterase and total flavonoid content. Whereas again the semi-determinate and indeterminate tomato genotypes along with the category hybrid with positive factor scores of 2.619, 0.252 and 0.114 in PC- II, exhibited better performance for the traits such as total soluble sugars, reducing sugars, chlorophyll b, malondialdehyde content, ascorbic acid, superoxide dismutase and peroxidase. Hybrid vigor was observed in the hybrids for investigated traits. The aforementioned tomato genotypes showing outstanding performance in the respective traits can be exploited in the breeding programs to improve nutritional quality of tomato that can further improve human health.

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ROS-scavenging-associated transcriptional and biochemical shifts during nectarine fruit development and ripening

Cited by 23 publications

References 64 publications

Integrated Physiological and Metabolomic Analyses Reveal the Differences in the Fruit Quality of the Blueberry Cultivated in Three Soilless Substrates

Integrated Physiological and Metabolomic Analyses Reveal the Differences in the Fruit Quality of the Blueberry Cultivated in Three Soilless Substrates

A D-cysteine desulfhydrase, SlDCD2, participates in tomato fruit ripening by modulating ROS homoeostasis and ethylene biosynthesis

Fruit nutritional composition, antioxidant and biochemical profiling of diverse tomato (Solanum lycopersicum L.) genetic resource

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