ROS Signalling in Plant Embryogenesis

Elhiti, Mohamed; Stasolla, Claudio

doi:10.1007/978-3-319-10079-1_10

Cited by 7 publications

(5 citation statements)

References 83 publications

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“…The intensive crosstalk between ROS and auxin has been reported to control the cell responsiveness to different developmental stimuli ( Tognetti et al, 2017 ). Accordingly, auxin-induced embryogenic cultures were observed to accumulate ROS ( Kairong et al, 1999 ; Elhiti and Stasolla, 2015 ; Cheng et al, 2015 , 2016 ), and similarly, we found the intensive production of superoxide anion (O 2 .- ) in the TSA-treated explants. It is noteworthy that ROS accumulation was not observed in the TSA-treated roots of Populus ( Ma et al, 2016 ) and this finding strengthens the assumption that ROS are produced in relation to the auxin accumulation rather than in response to the TSA treatment per se .…”

Section: Discussionsupporting

confidence: 66%

Trichostatin A Triggers an Embryogenic Transition in Arabidopsis Explants via an Auxin-Related Pathway

et al. 2018

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Auxin is an important regulator of plant ontogenies including embryo development and the exogenous application of this phytohormone has been found to be necessary for the induction of the embryogenic response in plant explants that have been cultured in vitro. However, in the present study, we show that treatment of Arabidopsis explants with trichostatin A (TSA), which is a chemical inhibitor of histone deacetylases, induces somatic embryogenesis (SE) without the exogenous application of auxin. We found that the TSA-treated explants generated somatic embryos that developed efficiently on the adaxial side of the cotyledons, which are the parts of an explant that are involved in auxin-induced SE. A substantial reduction in the activity of histone deacetylase (HDAC) was observed in the TSA-treated explants, thus confirming a histone acetylation-related mechanism of the TSA-promoted embryogenic response. Unexpectedly, the embryogenic effect of TSA was lower on the auxin-supplemented media and this finding further suggests an auxin-related mechanism of TSA-induced SE. Congruently, we found a significantly increased content of indolic compounds, which is indicative of IAA and an enhanced DR5::GUS signal in the TSA-treated explants. In line with these results, two of the YUCCA genes (YUC1 and YUC10), which are involved in auxin biosynthesis, were found to be distinctly up-regulated during TSA-induced SE and their expression was colocalised with the explant sites that are involved in SE. Beside auxin, ROS were extensively accumulated in response to TSA, thereby indicating that a stress-response is involved in TSA-triggered SE. Relevantly, we showed that the genes encoding the transcription factors (TFs) that have a regulatory function in auxin biosynthesis including LEC1, LEC2, BBM, and stress responses (MYB118) were highly up-regulated in the TSA-treated explants. Collectively, the results provide several pieces of evidence about the similarities between the molecular pathways of SE induction that are triggered by TSA and 2,4-D that involve the activation of the auxin-responsive TF genes that have a regulatory function in auxin biosynthesis and stress responses. The study suggests the involvement of histone acetylation in the auxin-mediated release of the embryogenic program of development in the somatic cells of Arabidopsis.

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Section: Discussionsupporting

confidence: 66%

Trichostatin A Triggers an Embryogenic Transition in Arabidopsis Explants via an Auxin-Related Pathway

et al. 2018

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“…Other pathways in this cluster included jasmonic acid and ethylene responses, and immune and defense responses. ROS have been reported to accumulate shortly after wounding and to participate in many stress responses [36, 37]. This explains why the first group of oxidative detoxification genes had co-expression relationships with genes involved in wounding and defense responses.…”

Section: Discussionmentioning

confidence: 99%

Gene co-expression network analysis reveals pathways associated with graft healing by asymmetric profiling in tomato

2019

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Background The ability of severed rootstocks and shoots to re-establish vascular connections is used to generate grafted plants that combine desirable traits from both scions and rootstocks. Clarifying the mechanisms of graft healing is essential for its further application. We performed RNA sequencing of internodes near the cut position, making a distinction between separated or grafted tissues above and below the cut, in order to obtain a genetic description of graft union formation. Results Using weighted gene co-expression analysis, variable transcripts were clustered into 10 distinct co-expression networks (modules) based on expression profiles, and genes with the most “hubness” (“hub” genes show the most connections in a network) within each module were predicted. A large proportion of modules were related to Position, and represent asymmetric expression networks from different pathways. Expression of genes involved in auxin and sugar transport and signaling, and brassinosteroid biosynthesis was increased above the cut, while stress response genes were up-regulated below the cut. Some modules were related to graft union formation, among which oxidative detoxification genes were co-expressed along with both wounding response and cell wall organization genes. Conclusions The present work provides a comprehensive understanding of graft healing-related gene networks in tomato. Also, the candidate pathways and hub genes identified here will be valuable for future studies of grafting in tomato. Electronic supplementary material The online version of this article (10.1186/s12870-019-1976-7) contains supplementary material, which is available to authorized users.

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“…In our study, NAA treatment had higher PPO activities compared with the control at the IL stage while had lower PPO activities at the CA and VB stage, potentially because the isolated layer disappeared, better formation of callus and the vascular bundle was beneficial to improve the healing speed and survival rate. As is well-known, ROS, the second messenger, regulates many biological processes, such as programmed cell death, cell cycle, and biotic or abiotic stress ( Elhiti & Stasolla, 2015 ). And the higher expression of genes involved in ROS played an vital to the healing of grafted tomatoes ( Xie, Dong & Shang, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis and physiological characteristics of exogenous naphthylacetic acid application to regulate the healing process of oriental melon grafted onto squash

Guo

et al. 2022

PeerJ

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The plant graft healing process is an intricate development influenced by numerous endogenous and environmental factors. This process involves the histological changes, physiological and biochemical reactions, signal transduction, and hormone exchanges in the grafting junction. Studies have shown that applying exogenous plant growth regulators can effectively promote the graft healing process and improve the quality of grafted plantlets. However, the physiological and molecular mechanism of graft healing formation remains unclear. In our present study, transcriptome changes in the melon and cucurbita genomes were analyzed between control and NAA treatment, and we provided the first view of complex networks to regulate graft healing under exogenous NAA application. The results showed that the exogenous NAA application could accelerate the graft healing process of oriental melon scion grafted onto squash rootstock through histological observation, increase the SOD, POD, PAL, and PPO activities during graft union development and enhance the contents of IAA, GA 3 , and ZR except for the IL stage. The DEGs were identified in the plant hormone signal-transduction, phenylpropanoid biosynthesis, and phenylalanine metabolism through transcriptome analysis of CK vs. NAA at the IL, CA, and VB stage by KEGG pathway enrichment analysis. Moreover, the exogenous NAA application significantly promoted the expression of genes involved in the hormone signal-transduction pathway, ROS scavenging system, and vascular bundle formation.

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ROS Signalling in Plant Embryogenesis

Cited by 7 publications

References 83 publications

Trichostatin A Triggers an Embryogenic Transition in Arabidopsis Explants via an Auxin-Related Pathway

Trichostatin A Triggers an Embryogenic Transition in Arabidopsis Explants via an Auxin-Related Pathway

Gene co-expression network analysis reveals pathways associated with graft healing by asymmetric profiling in tomato

Transcriptomic analysis and physiological characteristics of exogenous naphthylacetic acid application to regulate the healing process of oriental melon grafted onto squash

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