Rose bengal as a photosensitizer in the photodynamic therapy of breast cancer cell lines

Atenco-Cuautle, Juan Carlos; Delgado-López, María Guadalupe; Ramos-Garcı́a, R.; Ramírez-San-Juan, J. C.; Ramírez-Ramírez, J.; Spezzia-Mazzocco, Teresita

doi:10.1117/12.2525456

Cited by 5 publications

(7 citation statements)

References 12 publications

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“…Finally, judging from morphological changes shown in Figure 8D, FLTX2-induced cell death likely occurs though induction of apoptosis as suggested by the signs of chromatin condensation, intracellular vacuolation (black arrows), extensive membrane blebbing (blue arrows), cell shrinkage (red arrows), altered membrane integrity, aberrant morphology, and cellular fragmentation (green arrows), which collectively represent typical damage-associated patterns of extrinsic apoptosis or type I PCD (programmed cell death) [48,49]. In agreement with our findings, previous studies in cultured cells have shown that Rose Bengal acetate photodynamic therapy (RBAc-PDT) induces exposure and release of damage-associated molecular patterns (DAMPs) [50,51]. The interpretation of these findings is that irradiated FLTX2 leads to uncontrolled generation of ROS to levels where the intracellular antioxidant systems become overwhelmed and provoke irreversible oxidative stress.…”

Section: Photodynamic Effects Of Fltx2 On Cell Culturessupporting

confidence: 90%

FLTX2: A Novel Tamoxifen Derivative Endowed with Antiestrogenic, Fluorescent, and Photosensitizer Properties

Dı́az

Lobo

Hernández

et al. 2021

IJMS

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Tamoxifen is the most widely used selective modulator of estrogen receptors (SERM) and the first strategy as coadjuvant therapy for the treatment of estrogen-receptor (ER) positive breast cancer worldwide. In spite of such success, tamoxifen is not devoid of undesirable effects, the most life-threatening reported so far affecting uterine tissues. Indeed, tamoxifen treatment is discouraged in women under risk of uterine cancers. Recent molecular design efforts have endeavoured the development of tamoxifen derivatives with antiestrogen properties but lacking agonistic uterine tropism. One of this is FLTX2, formed by the covalent binding of tamoxifen as ER binding core, 7-nitrobenzofurazan (NBD) as the florescent dye, and Rose Bengal (RB) as source for reactive oxygen species. Our analyses demonstrate (1) FLTX2 is endowed with similar antiestrogen potency as tamoxifen and its predecessor FLTX1, (2) shows a strong absorption in the blue spectral range, associated to the NBD moiety, which efficiently transfers the excitation energy to RB through intramolecular FRET mechanism, (3) generates superoxide anions in a concentration- and irradiation time-dependent process, and (4) Induces concentration- and time-dependent MCF7 apoptotic cell death. These properties make FLTX2 a very promising candidate to lead a novel generation of SERMs with the endogenous capacity to promote breast tumour cell death in situ by photosensitization.

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Section: Photodynamic Effects Of Fltx2 On Cell Culturessupporting

confidence: 90%

FLTX2: A Novel Tamoxifen Derivative Endowed with Antiestrogenic, Fluorescent, and Photosensitizer Properties

Dı́az

Lobo

Hernández

et al. 2021

IJMS

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“…A previous study demonstrated that PDT with RB induces its anticancer effect through singlet oxygen-mediated cellular death [8]. RB is a well-known photosensitiser investigated for antibacterial [14], antifungal [15,16], anticancer [17], and tissue-bonding [18] applications. RB and its derivatives in solution exhibited cytotoxic activity against cancer cells [17,19]; for the treatment of hepatocellular cancer, RB in solution has also received approval from the US Food and Drug Administration [20].…”

Section: Introductionmentioning

confidence: 99%

“…RB is a well-known photosensitiser investigated for antibacterial [14], antifungal [15,16], anticancer [17], and tissue-bonding [18] applications. RB and its derivatives in solution exhibited cytotoxic activity against cancer cells [17,19]; for the treatment of hepatocellular cancer, RB in solution has also received approval from the US Food and Drug Administration [20]. However, being a hydrophilic photosensitiser, RB has a limited capacity to cross cellular membranes, therefore restricting its clinical application [21].…”

Section: Introductionmentioning

confidence: 99%

Photodynamic Treatment of Human Breast and Prostate Cancer Cells Using Rose Bengal-Encapsulated Nanoparticles

Uddin,

Bekmukhametova,

Antony

et al. 2023

Molecules

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Cancer, a prominent cause of death, presents treatment challenges, including high dosage requirements, drug resistance, poor tumour penetration and systemic toxicity in traditional chemotherapy. Photodynamic therapy, using photosensitizers like rose bengal (RB) with a green laser, shows promise against breast cancer cells in vitro. However, the hydrophilic RB struggles to efficiently penetrate the tumour site due to the unique clinical microenvironment, aggregating around rather than entering cancer cells. In this study, we have synthesized and characterized RB-encapsulated chitosan nanoparticles with a peak particle size of ~200 nm. These nanoparticles are readily internalized by cells and, in combination with a green laser (λ = 532 nm) killed 94–98% of cultured human breast cancer cells (MCF-7) and prostate cancer cells (PC3) at a low dosage (25 μg/mL RB-nanoparticles, fluence ~126 J/cm2, and irradiance ~0.21 W/cm2). Furthermore, these nanoparticles are not toxic to cultured human normal breast cells (MCF10A), which opens an avenue for translational applications.

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“…Rose Bengal (RB) is a highly effective and biocompatible photosensitiser commonly employed in PDT for microorganisms and cancer [ 19 , 20 , 21 , 22 , 23 ]. It has demonstrated successful eradication of triple-negative breast cancer cells and highly infiltrating ductal type T47D cells [ 24 ]. Yet, the clinical application of PDT using RB faces limitations due to the insufficient penetration of RB within the solid tumour microenvironment.…”

Section: Introductionmentioning

confidence: 99%

Encapsulated Rose Bengal Enhances the Photodynamic Treatment of Triple-Negative Breast Cancer Cells

Uddin,

Bekmukhametova,

Antony

et al. 2024

Molecules

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Among breast cancer subtypes, triple-negative breast cancer stands out as the most aggressive, with patients facing a 40% mortality rate within the initial five years. The limited treatment options and unfavourable prognosis for triple-negative patients necessitate the development of novel therapeutic strategies. Photodynamic therapy (PDT) is an alternative treatment that can effectively target triple-negative neoplastic cells such as MDA-MB-231. In this in vitro study, we conducted a comparative analysis of the PDT killing rate of unbound Rose Bengal (RB) in solution versus RB-encapsulated chitosan nanoparticles to determine the most effective approach for inducing cytotoxicity at low laser powers (90 mW, 50 mW, 25 mW and 10 mW) and RB concentrations (50 µg/mL, 25 µg/mL, 10 µg/mL and 5 µg/mL). Intracellular singlet oxygen production and cell uptake were also determined for both treatment modalities. Dark toxicity was also assessed for normal breast cells. Despite the low laser power and concentration of nanoparticles (10 mW and 5 µg/mL), MDA-MB-231 cells experienced a substantial reduction in viability (8 ± 1%) compared to those treated with RB solution (38 ± 10%). RB nanoparticles demonstrated higher singlet oxygen production and greater uptake by cancer cells than RB solutions. Moreover, RB nanoparticles display strong cytocompatibility with normal breast cells (MCF-10A). The low activation threshold may be a crucial advantage for specifically targeting malignant cells in deep tissues.

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Rose bengal as a photosensitizer in the photodynamic therapy of breast cancer cell lines

Cited by 5 publications

References 12 publications

FLTX2: A Novel Tamoxifen Derivative Endowed with Antiestrogenic, Fluorescent, and Photosensitizer Properties

FLTX2: A Novel Tamoxifen Derivative Endowed with Antiestrogenic, Fluorescent, and Photosensitizer Properties

Photodynamic Treatment of Human Breast and Prostate Cancer Cells Using Rose Bengal-Encapsulated Nanoparticles

Encapsulated Rose Bengal Enhances the Photodynamic Treatment of Triple-Negative Breast Cancer Cells

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