Roseibium litorale sp. nov., isolated from a tidal flat sediment and proposal for the reclassification of Labrenzia polysiphoniae as Roseibium polysiphoniae comb. nov.

Abstract

A novel Gram-stain-negative, facultatively anaerobic, rod-shaped and non-motile bacterial strain, designated as 4C16AT, was isolated from a tidal flat sediment and characterized by using a polyphasic taxonomic approach. Strain 4C16AT was found to grow at 10–40 °C (optimum, 28 °C), at pH 5.0–10.0 (optimum, pH 6.0–7.0) and in 0–6 % (w/v) NaCl (optimum, 1 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 4C16AT fell into the genus Roseibium , and shared the highest identity of 98.9 % with the closest type strain Roseibium suaedae KACC 13772T and less than 98.0 % identity with other type strains of recognized species within this genus. The phylogenomic analysis indicated that strain 4C16AT formed an independent branch within this genus. The 28.6 % digital DNA–DNA hybridization estimate and 85.0 % average nucleotide identity between strains 4C16AT and R. suaedae KACC 13772T were the highest, but still far below their respective threshold for species definition, implying that strain 4C16AT should represent a novel genospecies. The predominant cellular fatty acid was summed feature 8; the polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylmonomethylethanolamine; the respiratory quinones were Q-9 and Q-10. The genomic DNA G+C content was 59.8mol %. Based on phylogenetic analyses and phenotypic and chemotaxonomic characteristics, strain 4C16AT is concluded to represent a novel species of the genus Roseibium , for which the name Roseibium litorale sp. nov. is proposed. The type strain of the species is 4C16AT (=GDMCC 1.1932T=KACC 22078T). We also propose the reclassification of Labrenzia polysiphoniae as Roseibium polysiphoniae comb. nov. and ‘Labrenzia callyspongiae’ as Roseibium callyspongiae sp. nov.

Roseibium limicola sp. nov., isolated from tidal mudflat

“…[1], is affiliated with the family Rhodobacteraceae. At the time of writing, the genus Roseibium belongs to the class Alphaproteobacteria and includes 13 validly described species (https://lpsn.dsmz.de/genus/roseibium) widely isolated from diverse environments, such as sand [1], marine sediment [2, 3], fresh water [4], oysters [5], dinoflagellate [6], saline lake [7], tidal-flat sediment [8, 9], red algae [1, 10] and rhizosphere of sea plant [11], and annual sea plant [12]. The taxonomy of most members in the genus Roseibium is rod-shaped, Gram-stain-negative, motile, chemoheterotrophic and non-spore-forming, with ubiquinone-10 (Q-10) as the major respiratory quinone and summed feature 8 containing C 18 : 1 ω 7 c and/or C 18 : 1 ω 6 c as the major fatty acid.…”

Comparative Genomics Reveals Genetic Diversity and Metabolic Potentials of the Genus Qipengyuania and Suggests Fifteen Novel Species

“…Cellular and colony morphology, motility, Gram staining, and anaerobic growth of isolates were performed according to the methods described previously ( 78 ). Catalase and oxidase activities, the ranges and optima of temperature, pH, and NaCl, and the growth on different media were performed according to the methods described previously ( 79 ). The autotrophic growth was determined according to the method by Knittel et al ( 80 ) and using the strain Thiomicrospira sp.…”

Roseibium algicola sp. nov. and Roseibium porphyridii sp. nov., isolated from marine red algae

“…The genus Roseibium belonging to the family Stappiaceae of the phylum Pseudomonadota was first proposed by Suzuki et al [1] in 2000 to describe Roseibium denhamense and Roseibium hamelinense with Roseibium denhamense as the type species, and amended by Biebl et al [2]. At the time of this writing (December 2023), this genus includes 14 validly and one invalidly published species (https://lpsn.dsmz.de/genus/roseibium), which were isolated from mainly from marine environments, such as marine sediment [1,3], river estuary [4], oyster [5], saline lake [6], seawater [7], tidal-flat sediment [8,9], red algae [10] and sea plants [11,12]. Members of the genus Roseibium are Gram-stain-negative, aerobic, non-endospore-forming, motile or non-motile, oxidase-positive, rod-shaped bacteria having ubiquinone-10 (Q-10) as the major respiratory quinone, DNA G+C contents ranging from 56.5 to 64.8 mol%, and C 18 : 0 , C 18: 1 ω7c 11-methyl, C 20 : 1 ω7c and summed feature 8 (C 18 : 1 ω7c and/or C 18 : 1 ω6c) as major fatty acids [1][2][3][4][5][6][7][8][9][10][11][12].…”

“…At the time of this writing (December 2023), this genus includes 14 validly and one invalidly published species (https://lpsn.dsmz.de/genus/roseibium), which were isolated from mainly from marine environments, such as marine sediment [1,3], river estuary [4], oyster [5], saline lake [6], seawater [7], tidal-flat sediment [8,9], red algae [10] and sea plants [11,12]. Members of the genus Roseibium are Gram-stain-negative, aerobic, non-endospore-forming, motile or non-motile, oxidase-positive, rod-shaped bacteria having ubiquinone-10 (Q-10) as the major respiratory quinone, DNA G+C contents ranging from 56.5 to 64.8 mol%, and C 18 : 0 , C 18: 1 ω7c 11-methyl, C 20 : 1 ω7c and summed feature 8 (C 18 : 1 ω7c and/or C 18 : 1 ω6c) as major fatty acids [1][2][3][4][5][6][7][8][9][10][11][12]. In the course of our research on interactions between marine algae and bacteria, numerous novel bacteria have been isolated from marine red algae [13][14][15][16][17].…”