Rosemary essential oil enhances culture establishment and inhibits contamination and enzymatic browning: Applications for in vitro propagation of Aloe vera L.

Hamdeni, Imtinene; Slim, Slim; Sanaa, Adnen; Louhaichi, Mounir; Boulila, Abdennacer; Bettaieb, Taoufik

doi:10.1016/j.sajb.2021.06.009

Cited by 14 publications

(6 citation statements)

References 36 publications

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“…The use of plants' extracts and essential oils was reported to be capable of inhibiting microbial contamination effectively in Aloe vera, Fragaria ananassa, Phoenix dactylifera, Pennisetum purpureum, Manihot utilissima, and Zea mays cultures [13,[123][124][125]. In addition, the application of the CRISPR and TALENs methods was successful in producing disease-free plants in Oryza sativa, Solanum lycopersicum, and Malus domestica towards pathogenic mi-crobes such as Blumeria graminis, Oidium neolycopersici, and Magnaporthe oryzae [126][127][128][129].…”

Section: Challenges and Limitationsmentioning

confidence: 99%

“…Browning is usually caused by a variety of chemical reactions, such as caramelization, phenylalanine deamination, carbonyl amine reaction, o-quinone secondary reactions, lipid oxidation, and enzymatic oxidation [8,11,12]. Enzymatic browning of plant tissues causes unappealing changes in the explants and can cause explant death and failure of regeneration in explant culture for propagation [13], thus affecting both the survival and competitive ability of the explants.…”

Section: Introductionmentioning

confidence: 99%

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Managing Lethal Browning and Microbial Contamination in Musa spp. Tissue Culture: Synthesis and Perspectives

et al. 2023

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Browning and contamination are regarded as the main constraints in the plant tissue culture of Musa spp. that can hinder the success of plant propagation in vitro. Browning is caused by enzymatic reactions due to explant injury, while microbial contamination is caused by phyllospheric, rhizospheric, and endophytic microorganisms that reside on, in, and inside the plants. When not properly addressed, they can cause decreased regenerative ability, decreased callus growth, inhibited adventitious shoot growth, and even tissue death. To overcome the browning problem, various attempts have been made in vitro, e.g., immersing the explants in an anti-browning solution, incorporating anti-browning compounds into the medium, and manipulating cultural practices. Correspondingly, to control the problem of contamination, efforts have been made, for example, using various methods, such as thermotherapy, chemotherapy, and cryotherapy, and chemical agents, such as disinfectants, antiseptics, and nanoparticles. This review aims to investigate and provide a comprehensive understanding of the causes of browning and contamination as well as the many approaches used to control browning and contamination problems in Musa spp. tissue cultures.

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Section: Challenges and Limitationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Managing Lethal Browning and Microbial Contamination in Musa spp. Tissue Culture: Synthesis and Perspectives

et al. 2023

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“…Backer) tissue cultures (Ornellas et al, 2017 ), as well as in chrysanthemum, Anthurium , Hosta Jacq., raspberry, blackberry, and strawberry in vitro cultures (Orlikowska et al, 2015 ), with some success. Essential oils with antimicrobial properties have also been explored for use in controlling contamination in plant cultures (Hamdeni et al, 2022 ). Rosemary ( Salvia rosmarinus Schleid.)…”

Section: Establishing and Maintaining “Clean” In Vitro Culturesmentioning

confidence: 99%

“…and thyme ( Thymus vulgaris L.) essential oils were tested for their ability to inhibit microbial growth in Aloe vera L. shoot cultures. Although thyme essential oil was toxic to plant growth, rosemary essential oil was effective at controlling both microbial contamination and oxidative browning at the concentrations tested (Hamdeni et al, 2022 ).…”

Section: Establishing and Maintaining “Clean” In Vitro Culturesmentioning

confidence: 99%

Minimizing the deleterious effects of endophytes in plant shoot tip cryopreservation

et al. 2022

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Plant cryopreservation technologies are used within gene banks for the long‐term preservation of vegetatively propagated collections. Surface‐sterilized plant tissues grown in the field, greenhouse/screenhouse, growth chamber, or in vitro are the source of shoot tips subjected to vitrification‐based cryopreservation methods. Here, we describe the methods used to minimize microbial contamination during the tissue culture initiation process. We also discuss the occurrence and possible elimination of endophytes after extended in vitro culture and during recovery after liquid nitrogen exposure. We describe two case studies in which bacterial endophytes were observed in Citrus gene bank accessions during recovery after cryopreservation. These were identified using the MinION Oxford Nanopore system and Kirby–Bauer disc diffusion assays to examine the bacterial responses to antibiotic exposure. The methods used in this case study could be applied to identify endophytes to better target antimicrobial treatments of plant tissue collections.

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“…The particle size and polymer dispersity index (PDI) of different nanoparticles were measured by a laser particle size analyzer (Nano ZS90, Malvern Instruments, Worcester, UK). The standard curve of REO was obtained through GC-MS (6890 GC/5973 NMSD, Agilent, Palo Alto, CA, American) [37]. The initial temperature was 60 • C for 1 min, then the temperature was raised to 120 • C at 4 • C/min and raised to 150 • C at 10 • C/min for 5 min.…”

Section: Particle Size Pdi and Encapsulation Efficiency Of Nanoparticlesmentioning

confidence: 99%

A Novel Biocompatible Ternary Nanoparticle with High Antibacterial Activity: Synthesis, Characterization, and Its Application in Beef Preservation

Lin

Luo

Li³

et al. 2022

Foods

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Edible nanoparticles containing antibacterial agents are one of the effective strategies to control foodborne diseases. Herein, novel ternary nanoparticles (TNP) were prepared from rosemary essential oil (REO), nisin and Lycium barbarum polysaccharides (LBP) through hydrophobic and electrostatic interaction. The average particle size of TNP was 211.5 nm, and its encapsulation efficiency reached 86.6%. After the addition of LBP, the physical stability, thermal stability and storage stability of TNP were significantly improved. In vitro, compared with the control group, the population of S. aureus and E. coli O157:H7 in the TNP-treated group was reduced by 2.386 log CFU/mL and 1.966 log CFU/mL, respectively, on the fifth day. The free radical scavenging rate of TNP was 63.15%. The application of TNP on beef presented favorable preservation effects without affecting its color and texture. Therefore, the synthesis strategy of TNP has important reference significance for the research and development of new food antibacterial agents.

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Rosemary essential oil enhances culture establishment and inhibits contamination and enzymatic browning: Applications for in vitro propagation of Aloe vera L.

Cited by 14 publications

References 36 publications

Managing Lethal Browning and Microbial Contamination in Musa spp. Tissue Culture: Synthesis and Perspectives

Managing Lethal Browning and Microbial Contamination in Musa spp. Tissue Culture: Synthesis and Perspectives

Minimizing the deleterious effects of endophytes in plant shoot tip cryopreservation

A Novel Biocompatible Ternary Nanoparticle with High Antibacterial Activity: Synthesis, Characterization, and Its Application in Beef Preservation

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