Roseoloviruses: unmet needs and research priorities

Caserta, Mary T.; Krug, Laurie T.; Pellett, Philip E.

doi:10.1016/j.coviro.2014.10.005

Cited by 6 publications

(7 citation statements)

References 11 publications

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“…Fourteen (50%) studies reported research priorities to prevent mortality and manage other non‐graft‐related complications (including cancer, cardiovascular disease, diabetes, and infection) in transplant recipients . Some broad research priorities including minimizing side‐effects (e.g., diarrhea ) and organ damage were proposed .…”

Section: Resultsmentioning

confidence: 99%

“…Eight studies identified research priorities on the diagnosis, prevention and management of infections , which were mainly focused on viral infections (CMV , EBV , HCV ). Prevention of CMV through prophylactic therapy , determining “residual risk of late‐onset CMV disease” , and linking “viral load determinations with prediction of disease” were specified.…”

Section: Resultsmentioning

confidence: 99%

“…In liver transplantation, participants prioritized the effect of specific immunosuppressive regimens, interferon, and donor–recipient HLA matching on recurrence of HCV , “longitudinal histological studies” in patients with recurrent HCV to examine outcomes such as cirrhosis, and “maternal–fetal” HCV transmission . One study identified priorities on roseoloviruses and ciHHV‐6 .…”

Section: Resultsmentioning

confidence: 99%

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Research priority setting in organ transplantation: a systematic review

et al. 2017

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Barriers to access and long-term complications remain a challenge in transplantation. Further advancements may be achieved through research priority setting with patient engagement to strengthen its relevance. We evaluated research priority setting in solid organ transplantation and described stakeholder priorities. Databases were searched to October 2016. We synthesized the findings descriptively. The 28 studies (n = 2071 participants) addressed kidney [9 (32%)], heart [7 (25%)], liver [3 (11%)], lung [1 (4%)], pancreas [1 (4%)], and nonspecified organ transplantation [7 (25%)] using consensus conferences, expert panel meetings, workshops, surveys, focus groups, interviews, and the Delphi technique. Nine (32%) reported patient involvement. The 336 research priorities addressed the following: organ donation [43 priorities (14 studies)]; waitlisting and allocation [43 (10 studies)]; histocompatibility and immunology [31 (8 studies)]; immunosuppression [21 (10 studies)]; graft-related complications [38 (13 studies)]; recipient (non-graft-related) complications [86 (14 studies)]; reproduction [14 (1 study)], psychosocial and lifestyle [49 (7 studies)]; and disparities in access and outcomes [10 (4 studies)]. The priorities identified were broad but only one-third of initiatives engaged patients/caregivers, and details of the process were lacking. Setting research priorities in an explicit manner with patient involvement can guide investment toward the shared priorities of patients and health professionals.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Research priority setting in organ transplantation: a systematic review

et al. 2017

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“…Because the primer sequences used in the multiplex assay are not available, we cannot directly test this hypothesis. HHV-7 is commonly detected in human samples, usually in the absence of disease manifestations (49). Of the 4 samples with HHV-7 detected by sequencing, 3 were from the respiratory tract and 1 was whole blood.…”

Section: Discussionmentioning

confidence: 99%

Detection of Viruses in Clinical Samples by Use of Metagenomic Sequencing and Targeted Sequence Capture

Wylie

Buller

et al. 2018

J Clin Microbiol

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Metagenomic shotgun sequencing (MSS) is a revolutionary approach to viral diagnostic testing that allows simultaneous detection of a broad range of viruses, detailed taxonomic assignment, and detection of mutations associated with antiviral drug resistance. To enhance sensitivity for virus detection, we previously developed ViroCap, a targeted sequence capture panel designed to enrich nucleic acid from a comprehensive set of eukaryotic viruses prior to sequencing. To demonstrate the utility of MSS with targeted sequence capture for detecting clinically important viruses and characterizing clinically important viral features, we used ViroCap to analyze clinical samples from a diagnostic virology laboratory containing a broad range of medically relevant viruses. From 26 samples, MSS with ViroCap detected all of the expected viruses and 30 additional viruses. Comparing sequencing after capture enrichment with standard MSS, we detected 13 viruses only with capture enrichment and observed a consistent increase in the number and percentage of viral sequence reads as well as the breadth and depth of coverage of the viral genomes. Compared with clinical testing, MSS enhanced taxonomic assignment for 15 viruses, and codons associated with antiviral drug resistance in influenza A virus, herpes simplex virus (HSV), human immunodeficiency virus (HIV), and hepatitis C virus (HCV) could be analyzed. Overall, in clinical samples, MSS with targeted sequence capture provides enhanced virus detection and information of clinical and epidemiologic relevance compared with clinical testing and MSS without targeted sequence capture.

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“…However, human herpesvirus 6A (HHV-6A), HHV-6B, and HHV-7 (HHV-6A/6B/7) are highly related members of the less-well-characterized Roseolovirus genus of the human Betaherpesvirinae subfamily. HHV-6B is among the most prevalent human herpesviruses, but little is known about its pathogenesis, disease sequelae, or the host immune response (4).…”

mentioning

confidence: 99%

A Murine Herpesvirus Closely Related to Ubiquitous Human Herpesviruses Causes T-Cell Depletion

Patel

Zhao

Penna

et al. 2017

J Virol

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The human roseoloviruses human herpesvirus 6A (HHV-6A), HHV-6B, and HHV-7 comprise the Roseolovirus genus of the human Betaherpesvirinae subfamily. Infections with these viruses have been implicated in many diseases; however, it has been challenging to establish infections with roseoloviruses as direct drivers of pathology, because they are nearly ubiquitous and display species-specific tropism. Furthermore, controlled study of infection has been hampered by the lack of experimental models, and until now, a mouse roseolovirus has not been identified. Herein we describe a virus that causes severe thymic necrosis in neonatal mice, characterized by a loss of CD4 ϩ T cells. These phenotypes resemble those caused by the previously described mouse thymic virus (MTV), a putative herpesvirus that has not been molecularly characterized. By next-generation sequencing of infected tissue homogenates, we assembled a contiguous 174-kb genome sequence containing 128 unique predicted open reading frames (ORFs), many of which were most closely related to herpesvirus genes. Moreover, the structure of the virus genome and phylogenetic analysis of multiple genes strongly suggested that this virus is a betaherpesvirus more closely related to the roseoloviruses, HHV-6A, HHV-6B, and HHV-7, than to another murine betaherpesvirus, mouse cytomegalovirus (MCMV). As such, we have named this virus murine roseolovirus (MRV) because these data strongly suggest that MRV is a mouse homolog of HHV-6A, HHV-6B, and HHV-7.IMPORTANCE Herein we describe the complete genome sequence of a novel murine herpesvirus. By sequence and phylogenetic analyses, we show that it is a betaherpesvirus most closely related to the roseoloviruses, human herpesviruses 6A, 6B, and 7. These data combined with physiological similarities with human roseoloviruses collectively suggest that this virus is a murine roseolovirus (MRV), the first definitively described rodent roseolovirus, to our knowledge. Many biological and clinical ramifications of roseolovirus infection in humans have been hypothesized, but studies showing definitive causative relationships between infection and disease susceptibility are lacking. Here we show that MRV infects the thymus and causes T-cell depletion, suggesting that other roseoloviruses may have similar properties.KEYWORDS herpesviruses, roseolovirus T he study of herpesviruses has been fruitful, not only revealing mechanisms that drive disease but also providing insight into diverse processes, including oncogenesis and immune regulation (1). The Herpesviridae family includes several highly prevalent human pathogens (1-3). While they are related, these viruses have diverse genomes,

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Roseoloviruses: unmet needs and research priorities

Cited by 6 publications

References 11 publications

Research priority setting in organ transplantation: a systematic review

Research priority setting in organ transplantation: a systematic review

Detection of Viruses in Clinical Samples by Use of Metagenomic Sequencing and Targeted Sequence Capture

A Murine Herpesvirus Closely Related to Ubiquitous Human Herpesviruses Causes T-Cell Depletion

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