Roseomonas algicola sp. nov., isolated from a green alga, Pediastrum duplex

Abstract

A Gram-stain-negative, strictly aerobic bacterium, designated strain PeD5T, was isolated from a green alga Pediastrum duplex from the Nakdong river of the Republic of Korea. Cells were non-motile cocci, catalase-negative and oxidase-positive. Growth of PeD5T was observed at 25–40 °C (optimum, 35 °C) and pH 5.0–10.0 (optimum, pH 7–8), and in the presence of 0–0.25% (w/v) NaCl (optimum, 0%). PeD5T contained C16:0, C18:1ω7c 11-methyl, summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c) and summed feature 8 (comprising C18:1ω7c and/or C18:1ω6c) as major cellular fatty acids (>5%) and ubiquinone-10 as the sole isoprenoid quinone. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified phospholipid and an unidentified aminolipid were detected as major polar lipids. The genomic DNA G+C content of PeD5T was 71.0 mol%. PeD5T was most closely related to Roseomonas stagni HS-69T with a 97.6% 16S rRNA sequence similarity and shared less than 96.3% 16S rRNA sequence similarities with type strains of other species. Phylogenetic analysis based on 16S rRNA gene sequences indicated that PeD5T formed a phyletic lineage with Roseomonas stagni HS-69T within the genus Roseomonas . On the basis of results of phenotypic, chemotaxonomic and molecular analysis, strain PeD5T clearly represents a novel species of the genus Roseomonas , for which the name Roseomonas algicola sp. nov. is proposed. The type strain is PeD5T (=KACC 19925T=JCM 33309T).

Ramlibacter algicola sp. nov., isolated from a freshwater alga Cryptomonas obovoidea

“…The culture of C. obovoidea was homogenized and serially diluted using phosphate buffered saline (137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 2 mM KH 2 PO 4 , pH 7.2) and aliquots of each serially diluted sample were spread on R2A agar (BD). After aerobic incubation at 30 °C for 3 days, 16S rRNA genes of colonies grown on R2A agar were PCR-amplified using universal primers F1 (5′-AGA GTT TGA TCM TGG CTC AG-3′) and R13 (5′-TAC GGY TAC CTT GTT ACG ACT T-3′) and double-digested with Hae III and Hha I, as described previously [14]. Representative PCR amplicons showing distinct fragment patterns were selected and partially sequenced using the primer 340F (5′-CCT ACG GGA GGC AGC AG-3′) at Macrogen (Seoul, Republic of Korea).…”

“…The PCR-amplified 16S rRNA gene product of CrO1 T was further sequenced using the universal primers 518R (5′-ATT ACC GCG GCT GCT GG-3′) and 805F (5′-GAT TAG ATA CCC TGG TAG TC-3′) at Macrogen (Seoul, Republic of Korea) to obtain an almost complete 16S rRNA gene sequence, as described previously [14]. The sequences produced by 340F, 518R and 805F primers were assembled and 16S rRNA gene sequence similarities between CrO1 T and closely related type strains were calculated using the web-based tool of EzBioCloud server (www.ezbiocloud.net) [16].…”

“…Because it has been reported that some bacteria living in algal spheres can interact with and influence the growth of algae, alga-associated bacteria have gained great attention in order to understand algal growth and physiology [10]. Therefore, we also have isolated various alga-associated bacteria for studies on interactions between algae and bacteria [11][12][13][14]. In this study, a presumably novel strain belonging to the genus Ramlibacter, designated strain CrO1 T , was isolated from a freshwater alga Cryptomonas obovoidea and its taxonomic characteristics were analysed using a polyphasic approach.…”

Falsiroseomonas oryziterrae sp. nov., and Falsiroseomonas oryzae sp. nov., isolated from rice paddy soil

“…At the time of writing, the genus included 10 validly published species with correct names (https://lpsn.dsmz.de/genus/falsiroseomonas), and the type species is Falsiroseomonas stagni [3]. The type strains of this genus have been isolated from various environmental habitats, including green alga [4], arctic tundra soil [5], lake sediment [6], water-cooling system [7], freshwater [8,9], urban soil [10], pond water [11], agricultural soil [12] and a cooling tower [13]. We have been trying to isolate a variety of purple phototrophic bacteria (PPB) from rice paddy soil.…”

Roseomonas oleicola sp. nov., isolated from an oil production mixture in Yumen Oilfield, and emended description of Roseomonas frigidaquae

“…Roseomonas strains were initially isolated from human samples such as blood, wounds, sputum and exudates [1][2][3][4], but they were later isolated from other environmental samples such as air [5,6], soil [7][8][9][10], water [11,12], sediments [13,14], rhizosphere [15,16] and phyllosphere [17,18]. Recently, species of Roseomonas have also been isolated from aquatic sources, such as coral [19] and algae [20]. At the time of writing, the genus Roseomonas comprises 43 species and two subspecies that were validly published with correct names [21] (https:// lpsn.…”