Roseomonas elaeocarpi sp. nov., isolated from olive (Elaeocarpus hygrophilus Kurz.) phyllosphere

Abstract

An aerobic, Gram-stain-negative, coccobacillus-shaped, non-endospore-forming, pink-pigmented bacterium, designated PN2 T , was isolated from an olive leaf. The strain grew at 15-35 8C with an optimum temperature for growth at 30 8C, and at pH 5.0-7.5 with an optimum pH for growth at 6.0. Growth was observed in the presence of up to 1.02 % (w/v) NaCl. The major fatty acids were C 19 : 0 cyclo v8c, C 16 : 0 and C 18 : 1 v7c. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, unknown aminolipids, an unknown phospholipid and an unknown lipid. The respiratory quinone was ubiquinone-10. The DNA G+C content of strain PN2 T was 70.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PN2 T was closely related to members of the genus Roseomonas and shared highest similarity with Roseomonas mucosa ATCC BAA-692 T (96.5 %), Roseomonas gilardii subsp. gilardii ATCC 49956 T (96.2 %) and Roseomonas gilardii subsp. rosea ATCC BAA-691 T (96.2 %). Furthermore, the DNA-DNA relatedness value between strain PN2 T and the closest related species R. mucosa ATCC BAA-692 T was 27 %. These data allowed the phenotypic and genotypic differentiation of strain PN2 T from its closest phylogenetic neighbour (R. mucosa ATCC BAA-692 T ). Based on phenotypic and genotypic characteristics, strain PN2 T is classified as representing a novel species of the genus Roseomonas for which the name Roseomonas elaeocarpi sp. nov. is proposed. The type strain is PN2 T (5BCC 44864 T 5NBRC 107871 T ).Bacteria of the genus Roseomonas were first described by Rihs et al. (1993). They are Gram-stain-negative with plump coccus, coccobacillus or short rod shapes. Based on 16S rRNA gene sequence analysis, this genus belongs to the Alphaproteobacteria (Weyant & Whitney, 2005). At the time of writing, 23 species and two subspecies with validly published names are members of the genus Roseomonas, recently recognized species including Roseomonas aerophila (Kim et al., 2013) There have been reports on the isolation of Roseomonas species from various sources such as medical and environmental samples including plant phyllosphere. Together, these findings indicate that members of the genus Roseomonas exist widely in nature. Rihs et al. (1993) reported that the DNA G+C content of the genus Roseomonas ranges from 65 to 71 mol%. However, Kim et al. (2013) showed the DNA G+C content of R. aerophila was 73.0 mol%, slightly above values previously reported, and Dong et al. (2014) reported a 63.0 mol% DNA G+C content for R. alkaliterrae, a value that was slightly below values for species in the genus Roseomonas. From a literature review, the highest content for aThe GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain PN2 T is AB594202. major fatty acid of this genus was for C 18 : 1 v7c under the conditions studied (Gallego et al., 2006;Yoon et al., 2007;Furuhata et al., 2008Furuhata et al., , 2013Yoo et al., 2008;Zhang et al., 2008; Venkata Ramana et al., 2010;Lo...

Phylotaxogenomics for the Reappraisal of the Genus Roseomonas With the Creation of Six New Genera

“…Group I members were the only ones to have been isolated from a human blood sample (R. gilardii, R. mucosa, R. gilardii subsp. rosea) (Rihs et al, 1993;Han et al, 2003) except for R. elaeocarpi which was isolated from olive phyllosphere (Damtab et al, 2016). An examination of the literature demonstrated that the strains of Roseomonas have been widely reported in human infections and isolated from clinical samples like sputum, wounds, and genitourinary sites (Wallace et al, 1990;Rihs et al, 1993).…”

Roseomonas bella sp. nov., isolated from lake sediment

“…+, Positive; −, negative; nd, not determined. Reference data are from: a, Lee et al [25]; b, Rihs et al [5]; c, Damtab et al [26]; d, Kim et al [27].…”

Bacterial Contamination of Plant in vitro Cultures in Commercial Production Detected by High-Throughput Amplicon Sequencing

“…syringae. Roseomonas mucosa was detected among endophytic bacteria associated with Curcuma zedoaria (Sulistiyani et al, 2014), and Roseomonas species were found in banana (Nutaratat et al, 2013) and olive (Damtab et al, 2016) phyllosphere. To our knowledge, so far there are only two reports revealing a direct linkage of bacteria from genus Roseomonas to plant in vitro cultures (Pham et al, 2017;Barberini et al 2012).…”