Roseovarius arcticus sp. nov., a bacterium isolated from Arctic marine sediment

Abstract

An aerobic, Gram-stain-negative, motile, rod or long-rod-shaped bacterial isolate, strain MK6-18T, was isolated from a marine sediment sample from Kongsfjorden, Arctic. The bacterium grew optimally at 20 °C, pH 7.0 and in the presence of 1.0–2.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MK6-18T belonged to the genus Roseovarius . Its closest phylogenetic neighbour was Roseovarius nanhaiticus NH52JT showing 96.97 % 16S rRNA gene sequence similarity. The genome of strain MK6-18T is 4.2 Mb long in size with a G+C content of 59.5 mol%. The average nucleotide identity value between the genomes of strain MK6-18T and Roseovarius nanhaiticus NH52JT, was 78.0 %. Similar to other species of the genus Roseovarius , strain MK6-18T had ubiquinone 10 as the predominant ubiquinone and C12 : 0, C16 : 0, summed feature 3 (C16 : 1ω7c/ω6c) and summed feature 8 (C18 : 1ω7c/ω6c) as the major fatty acids. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine; one unidentified polar lipid, one unidentified aminolipid and one unidentified lipid were also detected. This is the first time that a member of the genus Roseovarius has been isolated from the Arctic, which may promote the study of the distribution characteristics and environmental adaptability of this genus. On the basis of the data provided here, strain MK6-18T should be classed as representing a novel species of the genus Roseovarius , for which the name Roseovarius arcticus sp. nov. is proposed. The type strain is MK6-18T (=CCTCC AB 2018219T=KCTC 72187T).

Roseovarius carneus sp. nov., a novel bacterium isolated from a coastal phytoplankton bloom in Xiamen

“…Members of the genus Roseovarius are catalase-positive, Gram-stain-negative, ovoid or rod-shaped, obligately aerobic bacteria. Most of Roseovarius type strains have been isolated from the marine environments such as coastal seawater [3], tidal flat [4], marine sediment [5], sponge [6] and whale bone [7]. By using high-throughput sequencing, a previous study found that the genus Roseovarius occurred in phytoplankton blooms and accounted for 0.15 % of the relative abundance of the bacterial community [8].…”

Draft Genome Sequence of Roseovarius sp. PS-C2, Isolated from Sekinchan Beach in Selangor, Malaysia

“…Roseovarius spp. are halophilic or halotolerant bacteria that thrive in various marine habitats ( 1 ). These bacteria have been reported as potential candidates for bioremediation and quorum sensing ( 2 , 3 ).…”

Pseudopuniceibacterium antarcticum sp. nov., isolated from an Antarctic marine sponge

“…Catalase activity was tested through bubble production in 3 % ( For fatty acid analysis, strain HQ09 T and the reference strain CY03 T were grown on marine agar 2216 plates at 25°C for 36 h. Fatty acid methyl esters were analysed by a Hewlett Packard 6890 N gas chromatograph and identified using the Sherlock Microbial Identification System (version 6.2). Polar lipids of strains HQ09 T and CY03 T were extracted and analysed by two-dimensional thin-layer chromatography (silica gel plates, layer thickness 0.2 mm; Merck) as described previously [31,32]. Both strains were cultivated in marine broth 2216 for respiratory quinone analysis.…”

“…Both strains were cultivated in marine broth 2216 for respiratory quinone analysis. Extraction and identification of respiratory quinones through HPLC (UltiMate 3000; Dionex) were performed as described previously [31].…”