Roseovarius bejariae sp. nov., a moderately halophilic bacterium isolated from a hypersaline steep-sided river bed

Abstract

<jats:p>An aerobic, Gram-stain-negative ovoid, designated as strain A21<jats:sup>T</jats:sup>, was isolated using the dilution-to-extinction method from a soil sample taken from Rambla Salada, an athalassohaline habitat located in Murcia (south-eastern Spain). Strain A21<jats:sup>T</jats:sup> is non-motile, has a respiratory metabolism and grows at NaCl concentrations within the range 0.5–15 % (w/v) [optimum, 5 % (w/v)], at 5–35 °C (optimum, 28 °C) and at pH 6–8 (optimum, pH 7.0). This strain is positive for catalase activity, oxidase activity and nitrate reduction. The 16S rRNA gene sequence indicates that it belongs to the genus <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1139" xlink:type="simple">Roseovarius</jats:ext-link> </jats:named-content> </jats:italic> in the class <jats:italic> <jats:named-content content-type="class"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.809" xlink:type="simple">Alphaproteobacteria</jats:ext-link> </jats:named-content> </jats:italic>. The most closely related species are <jats:italic>Roseovarius pacificus and Roseovarius halotolerans</jats:italic> to which the strain A21<jats:sup>T</jats:sup> shows 16S rRNA gene sequence similarity values of 98.06 and 97.7 %, respectively. The average nucleotide identity in <jats:sc>blast</jats:sc> and digital DNA–DNA hybridization values between strain A21<jats:sup>T</jats:sup> and <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.14288" xlink:type="simple">R. pacificus</jats:ext-link> </jats:named-content> </jats:italic> LMG 24575<jats:sup>T</jats:sup> are 76.8 and 21 %, respectively. The DNA G+C content based on the genome is 61.28 mol%. The major fatty acids (&gt;5 % of the total fatty acids) of strain A21<jats:sup>T</jats:sup> are C<jats:sub>18 : 1</jats:sub> ω7<jats:italic>c</jats:italic>/C<jats:sub>18 : 1</jats:sub> ω6<jats:italic>c</jats:italic> and C<jats:sub>16 : 0</jats:sub>. The only detected isoprenoid quinone in strain A21<jats:sup>T</jats:sup> is ubiquinone 10 (Q-10). The polar lipid profile contains phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and three unidentified polar lipids. Based on the phylogenetic, genotypic, phenotypic and chemotaxonomic data, the strain represents a novel species of the genus <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1139" xlink:type="simple">Roseovarius</jats:ext-link> </jats:named-content> </jats:italic>, for which the name <jats:italic>Roseovarius bejariae</jats:italic> sp. nov. is proposed. Strain A21<jats:sup>T</jats:sup> (=CECT 9817<jats:sup>T</jats:sup>=LMG 31311<jats:sup>T</jats:sup>) is the type strain.</jats:p>

Taxogenomic and Metabolic Insights into Marinobacterium ramblicola sp. nov., a New Slightly Halophilic Bacterium Isolated from Rambla Salada, Murcia

“…It is an extensive area formed by sedimentary materials in which, due to the low rainfall together with underground water that emerges, originates a number of streams and wadis. The prokaryotic community of Rambla Salada has been studied by our research group during recent decades [ 30 , 31 , 32 , 33 ], and we have described so far six novel halophilic bacterial species from this athalassohaline habitat: Idiomarina ramblicola [ 34 ], Halomonas cerina [ 35 ], Halomonas ramblicola [ 36 ], Blastomonas quesadae [ 37 ], Roseovarius ramblicola [ 38 ] and more recently, Roseovarius bejariae [ 39 ].…”

Cocaine esterase occurrence in global wastewater microbiomes and potential for biotransformation of novel psychoactive substances

“…Bacteria belonging to Streptomyces (Olanrewaju and Babalola, 2019) and Variovorax (Kim et al ., 2006) genera have been reported in soil. The genus Roseovarius was also found in soil and aquatic environment (Castro et al ., 2020), while the genus Rhizobium encompasses bacteria commonly encountered in soil and rhizosphere, such as Rhizobium favelukesii (Torres Tejerizo et al ., 2016). In turn, Komagataeibacter saccharivorans has been found in agroindustrial wastes (Abol‐Fotouh et al ., 2020) and the genus Listeria is ubiquitous, with L. monocytogenes well recognized as foodborne pathogen (Ranjbar and Halaji, 2018).…”