Roseovarius sediminilitoris sp. nov., isolated from seashore sediment

Abstract

A Gram-negative, motile and ovoid-to rod-shaped bacterial strain, designated M-M10 T , was isolated from a seashore sediment collected from the South Sea, South Korea. Strain M-M10 T grew optimally at pH 7.0-8.0, at 30 6C and in the presence of 2 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences revealed that strain M-M10 T clustered with the type strains of Roseovarius crassostreae, Roseovarius halocynthiae and Roseovarius marinus, with which it exhibited sequence similarities of 97.4, 97.3 and 95.1 %, respectively. It exhibited 93.2-95.1 % sequence similarity to the type strains of the other species of the genus Roseovarius. Strain M-M10 T contained Q-10 as the predominant ubiquinone and C 18 : 1 v7c and C 16 : 0 as the major fatty acids, as observed in the genus Roseovarius. The polar lipid profile of strain M-M10 T was similar to that of Roseovarius tolerans DSM 11457 T . The DNA G+C content of strain M-M10 T was 63.0 mol% and its mean DNA-DNA relatedness values with Roseovarius crassostreae DSM 16950 T and Roseovarius halocynthiae MA1-10 T were 16 % and 22 %, respectively. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, demonstrated that strain M-M10 T is distinct from other species of the genus Roseovarius. On the basis of the data presented, strain M-M10 T is considered to represent a novel species of the genus Roseovarius, for which the name Roseovarius sediminilitoris sp. nov. is proposed. The type strain is M-M10 T (5KCTC 23959 T 5CCUG 62413 T ).The genus Roseovarius, which belongs to the Alphaproteobacteria, was established by Labrenz et al. (1999) with Roseovarius tolerans, isolated from water samples from Ekho Lake, Antarctica, as the sole recognized species. At the time of writing, the genus Roseovarius comprises 11 species with validly published names (Euzéby, 1997). Members of the genus Roseovarius have been isolated from a hypersaline lake, seawater, a dinoflagellate culture, oysters, marine sediments and marine organisms (Labrenz et al. In this study, we describe a bacterial strain, designated M-M10 T resembling members of the genus Roseovarius, which was isolated from seashore sediment collected from Geoje island of the South Sea, South Korea. The aim of the present work was to establish the exact taxonomic position of strain M-M10 T by using a polyphasic characterization that included determination of phenotypic properties, a detailed phylogenetic investigation based on 16S rRNA gene sequences and genetic analysis.Strain M-M10 T was isolated by the standard dilution plating technique at 25 uC on marine agar 2216 (MA; BD) and cultivated routinely on MA at 30 u C. Roseovarius crassostreae DSM 16950 T and Roseovarius halocynthiae MA1-10 T were used as reference strains for DNA-DNA hybridization. Cell morphology, Gram reaction, growth under anaerobic conditions, in vivo pigment-absorption spectrum and susceptibility to antibiotics were determined as described previously (Jung et al., 2011). Strain M-M10 T was cultured at variou...

Roseovarius antarcticus sp. nov., isolated from a decayed whale bone

“…The genus Roseovarius , belonging to the class Alphaproteobacteria , with Roseovarius tolerans as the type species, was first described by Labrenz et al (1999). At the time of writing, the genus comprises 17 species with validly published names most of which were isolated from marine environments: Roseovarius litoreus (Jung et al , 2012), Roseovarius sediminilitoris (Park & Yoon, 2013) and Roseovarius marisflavi (Li et al , 2013). Some strains belonging to R. tolerans formed bacteriochlorophyll a (Bchl a ) and others did not, and two only formed photosynthetic pigments after six years of cultivation (Labrenz et al , 1999).…”

Roseovarius lutimaris sp. nov., isolated from a marine tidal flat

“…The genus Roseovarius accommodates catalase-positive, ovoid or rodshaped, obligately aerobic bacteria and, to date, all Roseovarius type strains have been retrieved from marine environments such as tidal flats, seawater, sea squirts and oysters. At the time of writing, the genus Roseovarius included 13 species with validly published names: R. tolerans (Labrenz et al, 1999), R. nubinhibens (González et al, 2003), R. mucosus (Biebl et al, 2005), R. crassostreae (Boettcher et al, 2005), R. aestuarii (Yoon et al, 2008), R. halotolerans (Oh et al, 2009), R. pacificus (Wang et al, 2009), R. nanhaiticus (Wang et al, 2010), R. indicus (Lai et al, 2011), R. marinus (Jung et al, 2011), R. halocynthiae (Kim et al, 2012b), R. litoreus (Jung et al, 2012) and R. sediminilitoris (Park & Yoon, 2013). Tidal flats, called getbol in Korean, are broad, low-gradient, coastal muddy marshes that play important roles in habitat restoration and nutrient recycling (Winberg et al, 2007;Math et al, 2012).…”

Pseudoroseovarius zhejiangensis gen. nov., sp. nov., a novel alpha-proteobacterium isolated from the chemical wastewater, and reclassification of Roseovarius crassostreae as Pseudoroseovarius crassostreae comb. nov., Roseovarius sediminilitoris as Pseudoroseovarius sediminilitoris comb. nov. and Roseovarius halocynthiae as Pseudoroseovarius halocynthiae comb. nov.

“…At the time of writing, according to LPSN (Parte 2014; http://www.bacterio.net/index.html), the genus Roseovarius contains 18 species with validly published names. Members of the genus Roseovarius have been isolated from seawater, oysters, marine sediments, a sea squirt, a hypersaline lake, an amphioxus breeding zone and a dinoflagellate culture (Biebl et al 2005;Boettcher et al 2005;Kim et al 2012b;Labrenz et al 1999;Li et al 2013;Park and Yoon 2013;Yoon et al 2008). However, based on this and former studies, it is clear that the genus Roseovarius is polyphyetic and in need of revision (Lucena et al 2014;Park et al 2014;Rajasabapathy et al 2014).…”