Rosette Tests Following Thymectomy

Sandilands, G. P.; Gray, Kathleen; Cooney, Anne; Anderson, John R.; Simpson, J. A.; Behan, Peter O.

doi:10.1016/s0140-6736(75)91479-8

Cited by 19 publications

(6 citation statements)

References 5 publications

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“…This reaction was transsitory and the values returned gradually to preoperative levels. Similar findings have also been reported by Fritze and Herrmann (1975), Sandilands et al (1975), and Pudifin and Cox (1976). Bjorkholm et al (1975) found that a significant decrease in the number of T lymphocytes might appear several years after thymectomy.…”

Section: Discussionsupporting

confidence: 88%

Lymphocyte subpopulations in thymus and blood from patients with myasthenia gravis.

Aarli¹,

Heimann²,

Matre³

et al. 1979

Journal of Neurology, Neurosurgery & Psychiatry

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S U M M A R Y Cell suspensions were prepared from hyperplastic thymic tissue and lymphoepithelioma from patients with myasthenia gravis and from presumed normal thymic tissue obtained at cardiac surgery. The mononuclear cells were examined for surface markers. The mean percentages of both T lymphocytes and Fc receptor-carrying lymphocytes were similar in the three groups, whereas there was an increase in C receptor-carrying lymphocytes in the samples from myasthenic patients. Sections from the thymus gland were examined for T and B markers. In the hyperplastic thymus and in lymphoepithelioma, the T lymphocytes were distributed diffusely throughout the cortex and the medulla; in the normal thymus they were predominant in the cortex. The mean percentage of T and B lymphocytes in peripheral blood from patients with myasthenia gravis was normal. Thymectomy involved a transitory decrease in T lymphocytes with a corresponding increase in B lymphocytes.The rapid variation of the muscular weakness in myasthenia gravis is commonly ascribed to the blocking effect of circulating antibodies against the acetylcholine receptor protein of the muscle endplate (Lindstrom et al., 1976), while the long-term structural changes may also be caused by the effect of sensitised lymphocytes (Richman et al., 1976

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Section: Discussionsupporting

confidence: 88%

Lymphocyte subpopulations in thymus and blood from patients with myasthenia gravis.

Aarli¹,

Heimann²,

Matre³

et al. 1979

Journal of Neurology, Neurosurgery & Psychiatry

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“…One, however, has to be particularly cautious in interpreting results obtained in the human because of the possibility of nonspecific autologous rosette formation as mentioned above with reference to human spontaneous sheep cell rosettes (17), as well as rosette formation by antierythrocyte auto-antibody-forming cells, especially in autoimmune conditions. In fact, several authors have indeed found much higher A-RFC levels in man than in mice, both in thymus (24,26) and in peripheral blood (26,27). It may be that mouse and human lymphocytes differ in their binding of autologous erythrocytes as they do for their binding of sheep erythrocytes (17).…”

Section: Discussionmentioning

confidence: 98%

Binding of autologous erythrocytes to immature T-cells.

Charreire¹,

Bach²

1975

Proc. Natl. Acad. Sci. U.S.A.

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A small percentage of normal mouse thymus and spleen lymphocytes form rosettes with autologous erythrocytes. The number of these autologous rosettes increases 15-to 20-fold after adult thymectomy and to a lesser degee with aging. Autologous rosette level is also abnormally high in nude (congenitally athymic) mice. The high level of autolo gous rosette-forming celmsfound after adult thymectomy is normalized by injecting ng amounts of purified circulating thymic factor. Autologous rosette-forming cells adhere to nylon, belong to the less dense spleen cells, are in majority steroid-resistant in the thymus. All these properties suggest that autologous rosette-forming cells might belong to immature T-cell (thymic-dependent cell) precursors. Thymic-dependent cell (T-cell) maturation involves several steps from the primitive stem cell to the fully competent peripheral T-cell (1-5). The difficulties in visualizing these different steps come from the absence of morphological criteria available for other blood cell lineages or of markers used to distinguish B-and T-cells.A small percentage of normal mouse lymphoid cells bind autologous erythrocytes, forming rosettes. These autologous rosette-forming cells (A-RFC) are found predominantly in the thymus and at lower percentages in the spleen (6, 7). A-RFC have elicited a great interest because of their potential use as a model of self-recognition (6, 7). We report here that A-RFC level in the spleen is dramatically increased after adult thymectomy and that purified thymic hormone corrects such increase.These data and others, also presented here, suggest that autologous rosette formation may be a selective property of immature T-cells which could eventually be used as a marker of this cell population.MATERIAL AND METHODS Mice. Male and female mice of the CBA-H (histocompatibility type H-2k) or C57BI/6 (H-2b) strains were used in most experiments. In some cases, nude (congenitally athymic) mice were also utilized. When not specified, the mice were 6-8 weeks old. Thymectomies were performed by suction; "thymus-deprived" mice were prepared by thymectomy followed by irradiation (850 rads) and reconstitution by intravenous (i.v.) injection of 15 X 106 syngeneic bone marrow cells.Antitheta Serum (AOS). This was prepared by injecting 107 CBA thymocytes intraperitoneally (i.p.) into AKR mice once weekly for 8 weeks (5).

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“…Peripheral lymphocyte counts have been found to be normal in patients with MG [29], In an earlier study, non-thymectomized patients with MG had a T cell number within the normal range in the peripheral blood [30], A reduction in the number of T cells was noticed after adult thymectomy in one study [11], but not in another [31], In the present investigation, normal absolute lymphocyte counts were found in the blood of most thymectomized and non-thymectomized patients. The percentage of T cells in the peripheral blood was also found to be within the normal range in both patient groups, using the sheep red cell rosette tech nique.…”

Section: Discussionmentioning

confidence: 99%

Effect of Adult Thymectomy on the Development of 1-Chloro-2,4-Dinitrobenzene Contact Sensitivity and Other T Lymphocyte Functions in Patients with Myasthenia gravis

Roupe¹,

Lindholm²,

Hanson³

1980

Int Arch Allergy Immunol

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1-Chloro-2,4-dinitrobenzene (DNCB) contact sensitivity and other T lymphocyte functions were studied in thymectomized and non-thymectomized patients with myasthenia gravis. The ability to develop contact sensitivity was reduced in patients with myasthenia gravis and was further reduced after thymectomy. Memory lymphocyte function, as measured by skin tests with common microbial antigens, was intact. A positive phytohemagglutinin (PHA) skin test was found mainly in those patients who also developed contact sensitivity to DNCB. The number of rosette-forming T cells in the peripheral blood as well as mitogen stimulation with PHA was found to be normal in thymectomized as well as non-thymectomized patients. In the thymectomized group, mitogen stimulation with concanavalin A and staphylococcal protein A was also within the normal range, while increased stimulation was obtained with pokeweed mitogen (PWM) and purified protein derivative (PPD). No alteration in mixed lymphocyte culture reactivity or immunoglobulin levels was obtained compared with healthy blood donors. On the basis of these results, it is concluded that non-thymectomized as well as thymectomized patients with myasthenia gravis may have a defective subpopulation of T cells possibly residing in the T_H1-positive population. Furthermore, the increased lymphocyte stimulation obtained with PPD and PWM may indicate a reduction of suppressor cell activity after thymectomy.

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Rosette Tests Following Thymectomy

Cited by 19 publications

References 5 publications

Lymphocyte subpopulations in thymus and blood from patients with myasthenia gravis.

Lymphocyte subpopulations in thymus and blood from patients with myasthenia gravis.

Binding of autologous erythrocytes to immature T-cells.

Effect of Adult Thymectomy on the Development of 1-Chloro-2,4-Dinitrobenzene Contact Sensitivity and Other T Lymphocyte Functions in Patients with Myasthenia gravis

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