Rosettes integrity protects Plasmodium vivax of being phagocytized

Albrecht, Letusa; Lopes, Stefanie Costa Pinto; Silva, Ana Beatriz Iung Enembreck da; Barbosa, Vanessa; Almeida, Rodrigo Pm de; Siqueira, André Machado; Leite, Juliana Almeida; Bittencourt, Najara; Santos, Hellen Geremias dos; Bourgard, Catarina; Garcia, Luiz Fernando Cardoso; Kayano, Ana Carolina Andrade Vitor; Soares, Irene S.; Russell, Bruce; Rénia, Laurent; Lacerda, Marcus; Costa, Fabio T. M.

doi:10.1038/s41598-020-73713-w

Cited by 14 publications

(18 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, higher rosetting rates were recorded in the phagocytosis assay, suggesting that the parasites possess capacity of forming more rosettes. Functionally, rosetting of P. knowlesi -IRBC with URBC hampered phagocytosis of IRBC, which agreed well with earlier findings with P. falciparum and P. vivax parasites ( Albrecht et al, 2020 ; Lee et al, 2020 ). Rosetting reduced the phagocytosis of P. knowlesi -IRBC by ∼38%.…”

Section: Discussionsupporting

confidence: 91%

Cytoadherence Properties of Plasmodium knowlesi-Infected Erythrocytes

et al. 2022

Self Cite

View full text Add to dashboard Cite

Plasmodium knowlesi is responsible for zoonotic malaria infections that are potentially fatal. While the severe pathology of falciparum malaria is associated with cytoadherence phenomena by Plasmodium falciparum-infected erythrocytes (IRBC), information regarding cytoadherence properties of P. knowlesi-IRBC remained scarce. Here, we characterized the cytoadherence properties of RBC infected with the laboratory-adapted P. knowlesi A1-H.1 strain. We found that late-stage IRBC formed rosettes in a human serum-dependent manner, and rosettes hampered IRBC phagocytosis. IRBC did not adhere much to unexposed (unstimulated) human endothelial cell lines derived from the brain (hCMEC/D3), lungs (HPMEC), and kidneys (HRGEC). However, after being “primed” with P. knowlesi culture supernatant, the IRBC-endothelial cytoadherence rate increased in HPMEC and HRGEC, but not in hCMEC/D3 cells. Both endothelial cytoadherence and rosetting phenomena were abrogated by treatment of P. knowlesi-IRBC with trypsin. We also found that different receptors were involved in IRBC cytoadherence to different types of endothelial cells. Although some of the host receptors were shared by both P. falciparum- and P. knowlesi-IRBC, the availability of glycoconjugates on the receptors might influence the capacity of P. knowlesi-IRBC to cytoadhere to these receptors.

show abstract

Section: Discussionsupporting

confidence: 91%

Cytoadherence Properties of Plasmodium knowlesi-Infected Erythrocytes

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…As recently observed in several studies, P. vivax can adhere to endothelial receptors and form rosettes in a similar fashion as P. falciparum. (36,37) However, unlike P. falciparum, PfEMP1 and RIFIN antigens are absent in P. vivax. Therefore, some other antigens must be implicated in the adhesion phenomena.…”

Section: Discussionmentioning

confidence: 99%

Antigenicity and adhesiveness of a Plasmodium vivax VIR-E protein from Brazilian isolates

Schappo

Bittencourt

Bertolla

et al. 2021

Mem. Inst. Oswaldo Cruz

Self Cite

View full text Add to dashboard Cite

BACKGROUND Plasmodium vivax, the major cause of malaria in Latin America, has a large subtelomeric multigene family called vir. In the P. vivax genome, about 20% of its sequences are vir genes. Vir antigens are grouped in subfamilies according to their sequence similarities and have been shown to have distinct roles and subcellular locations. However, little is known about vir subfamilies, especially when comes to their functions. OBJECTIVE To evaluate the diversity, antigenicity, and adhesiveness of Plasmodium vivax VIR-E.METHODS Vir-E genes were amplified from six P. vivax isolates from Manaus, North of Brazil. The presence of naturally acquired antibodies to recombinant PvBrVIR-E and PvAMA-1 was evaluated by ELISA. Binding capacity of recombinant PvBrVIR-E was assessed by adhesion assay to CHO-ICAM1 cells.FINDINGS Despite vir-E sequence diversity, among those identified sequences, a representative one was chosen to be expressed as recombinant protein. The presence of IgM or IgG antibodies to PvBrVIR-E was detected in 23.75% of the study population while the presence of IgG antibodies to PvAMA-1 antigen was 66.25% in the same population. PvBrVIR-E was adhesive to CHO-ICAM1.MAIN CONCLUSIONS PvBrVIR-E was antigenic and adhesive to CHO-ICAM1.

show abstract

“…Marın-Menendez et al stated that rosetting was the cytoadherence phenotype of P. vivax infection ( 21 ). The adhesive force of infected erythrocyte by P.vivax forms a rosette as strong as P. falciparum characteristic, but the specific mechanism of rosette formation in P. vivax malaria is unestablished ( 22 ).…”

Section: Discussionmentioning

confidence: 99%

“…However, the adhesion phenotypes regarding immunoglobulin components have not been untangled yet. The rosetting was also related to Interleukin (IL)-6, IL-10, and IgM ( 22 ). Rosetting in P. vivax infection may play an essential role in the sequestration process, which elucidates parasite subpopulation in a specific organ, induces prolonged infection by the significant protection from the host immune system, and becomes a latent infection.…”

Section: Discussionmentioning

confidence: 99%

The Relationship Between Fetal Weight with Sequestration of Infected Erythrocyte, Monocyte Infiltration, and Malaria Pigment Deposition in Placenta of Mother Giving Birth Suffering from Plasmodium Vivax Infection

Prasetyorini

Erwan

Utomo³

et al. 2021

Med Arch

View full text Add to dashboard Cite

Background: Malaria in pregnancy can cause fatal complications by parasite sequestration mechanism, which can cause monocyte infiltration in the intervillous space. P. vivax infection was significantly associated with malaria pigment in the placenta, indicating past subclinical infections Objective: This study aimed to determine the mechanism of P. vivax in the pathogenesis of placental malaria and its relationship with LBW. Methods: This study was observational analytic with a cross-sectional approach. Placental tissue samples were obtained from pregnant women with LBW babies during delivery in Maumere, Nusa Tenggara Timur. The samples used in this study were confirmed by a polymerase chain reaction and consisted of 25 samples with 12 positive and 13 negative samples. Placental tissue samples were made with Hematoxylin-Eosin staining and observed under 1000x magnification at 100 fields using a light microscope. Parasite density, monocyte infiltration, and parasite pigments deposition were calculated. Results: Microscopic observation revealed that there was a significant difference in infected erythrocytes sequestration between groups. Interestingly, monocyte and malaria pigments accumulation were found in malaria-positive and -negative groups, and no significant difference between groups. The correlation test showed no significant relationship between monocyte infiltration and LBW in the malaria-positive and -negative group and between parasite pigments and LBW in both groups. Moreover, there was no significant correlation between parasite density and LBW in the positive and negative groups. Conclusion: P. vivax infection causes acute, sub-acute, and chronic placental malaria in subclinical infected pregnant women in Maumere, Nusa Tenggara Timur that might cause an LBW baby.

show abstract

Rosettes integrity protects Plasmodium vivax of being phagocytized

Cited by 14 publications

References 38 publications

Cytoadherence Properties of Plasmodium knowlesi-Infected Erythrocytes

Cytoadherence Properties of Plasmodium knowlesi-Infected Erythrocytes

Antigenicity and adhesiveness of a Plasmodium vivax VIR-E protein from Brazilian isolates

The Relationship Between Fetal Weight with Sequestration of Infected Erythrocyte, Monocyte Infiltration, and Malaria Pigment Deposition in Placenta of Mother Giving Birth Suffering from Plasmodium Vivax Infection

Contact Info

Product

Resources

About