Rosmarinic Acid Accumulation and Antioxidant Potential of Dracocephalum moldavica L. Cell Suspension Culture

Abstract

Dracocephalum moldavica L. (Lamiaceae) is known for its medicinal properties; however greater yields can potentially be achieved by in vitro cultivation. A cell suspension culture of D. moldavica L. (Lamiaceae) derived from root-derived callus was established in liquid MS medium supplemented with 2,4-D 0.5 mg/l and BAP 0.2 mg/l. The biomass and rosmarinic acid (RA) content were analyzed during the 15-day growth cycle of the culture. The highest fresh and dry weight (14.29 g/flask and 1.14 g/flask, respectively) and RA level (27.2 mg/g DW) were reached at day 12 of culture. Methanolic extracts of the culture were assayed for total phenolic content using the Folin-Ciocalteau method, and antioxidant activities using three in vitro tests: ABTS radical scavenging, ferric ion reduction (FRAP) and lipid peroxidation (LPO). RA content and antioxidant potential were found to be higher in cell suspension culture than in root-derived callus. The cell suspension culture also exhibited higher concentrations of RA and ABTS radical scavenging activity than those of the aerial parts of six-month-old field-grown plants of D. moldavica. The overall results show a significant correlation between antioxidant activity, total phenolic content and RA content of the examined extracts. The study presents for the first time the use of cell cultures of D. moldavica for production of therapeutically-valuable metabolites. Our results suggest that the obtained culture could be considered as a potential source of rosmarinic acid, a compound known for its strong antioxidant activity.

UPLC–PDA‐ESI–QTOF–MS/MS and GC‐MS analysis of Iranian Dracocephalum moldavica L.

“…TFC was in the range from 23.9 ± 1.2 (in aqueous extract) to 79.3 ± 2.5 mg QE g ‐1 (in EtOAc extract). In the literature, the antioxidant activity and TPC of a 70% aqueous MeOH extract of D. moldavica was evaluated by (Weremczuk‐jeżyna et al., 2017 ). The TPC of the aerial parts of D. moldavica was 110.1 mg GAE g ‐1 , which was higher than observed in our study.…”

Development of high-performance thin layer chromatography method for identification of phenolic compounds and quantification of rosmarinic acid content in some species of the Lamiaceae family

“…High content of RA (27.2mg/g) was also present in 70% methanol extracts of cell suspension of D. moldavica herb, and this species was estimated as a good candidate for in vitro production of this compound. [ 27 ] The highest level of RA content was also established for the herbal infusion of D. moldavica among the other species of Nepetoideae subfamily by HPLC. [ 8 ] High content of this compound was also revealed in the aerial parts of some Salvia species.…”

“…It had been reported that high content of phenolic compounds as well as aromatic components in essential oils is well correlated with the antioxidant activity of plant materials of Lamiaceae representatives. [ 4 5 , 9 27 , 29 30 ] Therefore, according to the foregoing, investigated herbs have a potent antioxidant property due to high content of RA, and the presence of other spots of polyphenols in HPTLC chromatograms.…”

LED illumination and plant growth regulators’ effects on growth and phenolic acids accumulation in Moluccella laevis L. in vitro cultures

“…Many of the Lamiaceae species have been used for studying the production of phenolic compounds in various in vitro systems, such as cell suspension (Weremczuk-Jeżyna et al 2017), callus and shoot culture (Karakas and Turker 2016) or hairy roots (Grzegorczyk-Karolak et al 2018). Usually, the yields were higher than in the shoot culture of M. laevis in our study.…”