Rosmarinic acid exerts a neuroprotective effect in the kainate rat model of temporal lobe epilepsy: Underlying mechanisms

Khamse, Safoura; Sadr, Seyed Shahabeddin; Roghani, Mehrdad; Hasanzadeh, Gholamreza; Mohammadian, Maryam

doi:10.3109/13880209.2015.1010738

Cited by 64 publications

(40 citation statements)

References 39 publications

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“…High antioxidant activities including SOD, catalase and GSH have been detected in the brain in order to cope with oxidative stress damage . In animals with liver injury induced by ischaemia reperfusion, Hup‐A treatment elevated the activity of catalase, SOD, and GSH, but diminished the MDA concentration and thereby improved the hepatic ischaemia reperfusion injury in rats .…”

Section: Discussionmentioning

confidence: 99%

Huperzine A ameliorates cognitive dysfunction and neuroinflammation in kainic acid‐induced epileptic rats by antioxidant activity and NLRP3/caspase‐1 pathway inhibition

Mohseni‐Moghaddam

Sadr

Roghani

et al. 2019

Clin Exp Pharma Physio

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Epilepsy is one of the most serious neurological problems, characterized by frequent spontaneous seizures. It is always accompanied with a multitude of difficulties, such as behavioural, cognitive, and psychiatric disturbances. 1,2 Temporal lobe epilepsy (TLE) is one of the most recognized kinds of epilepsy in humans.Abnormal structural changes in the hippocampus referred to as hippocampal sclerosis and neurodegeneration are associated with TLE. 3 Unilateral intrahippocampal administration of kainic acid provides a well-specified model of TLE, leading to oxidative damage and mitochondrial malfunction in brain tissue, especially in the limbic system 4 with subsequent development of devastating visuospatial memory loss. 5 Cognitive deficit, particularly learning and memory deterioration, is a major controversial issue and the most frequent neuropsychological feature in TLE patients. 6 Brain inflammation enhances neuronal excitability, promotes the development of seizures and is presumably related to the synaptic and

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Section: Discussionmentioning

confidence: 99%

Huperzine A ameliorates cognitive dysfunction and neuroinflammation in kainic acid‐induced epileptic rats by antioxidant activity and NLRP3/caspase‐1 pathway inhibition

Mohseni‐Moghaddam

Sadr

Roghani

et al. 2019

Clin Exp Pharma Physio

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“…Additionally, evidence has demonstrated the significant increase in the expression of the M1 microglial marker iNOS in a model of epilepsy induced by kainate in mice that causes severe damage to brain tissue [51] . Thus, we believe that the protective effect presented by RA in several experiments about neurodegenerative disorders, including the reduction of kainate-induced seizures [35] , may be caused by modulation of the M1-M2 microglial states, as shown here.…”

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confidence: 89%

“…Several biological activities are attributed to RA, such as antioxidant and anti-inflammatory properties and neuroprotective effects [26][27][28][29][30] . RA has been proven to be effective in experimental models of neurodegenerative diseases such as Alzheimer disease and amyotrophic lateral sclerosis [31,32] , and recently it was shown to have the interesting effect of reducing seizures in rats [27,[33][34][35] . A previous study showed that RA inhibits the activity of GABA-T.…”

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confidence: 99%

Rosmarinic Acid Attenuates the Activation of Murine Microglial N9 Cells through the Downregulation of Inflammatory Cytokines and Cleaved Caspase-3

Coelho

Viau²,

Staub³

et al. 2017

Neuroimmunomodulation

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Objective: The present study evaluated the ability of rosmarinic acid (RA) to inhibit microglia activation induced by lipopolysaccharide (LPS) in the N9 murine microglial cell line, and investigated the putative mechanisms involved in this process. Methods: In all tests, N9 murine microglial cells were pretreated with RA (0.1, 1.0, and 10 μM) for 20 h and exposed to LPS (1 μM/mL) for 4 h. Cell viability was measured by Trypan blue exclusion assay. Flow cytometry was used to detect reactive oxygen species (ROS), quantify cleaved caspase-3, and analyze the mitochondrial electrochemical potential. iNOS, Arg-1, TNF-α, IL-1β, and IL-6 proteins were analyzed by Western blotting, and their antigens were detected using the chemiluminescence technique. The effect of RA on DNA was evaluated by the Comet assay. Results: RA attenuated the expression of the M1 marker iNOS and the levels of proinflammatory factors, including TNF-α, IL-1β, and IL-6; it increased the expression of the M2 marker Arg-1, and inhibited, at least in part, ROS generation and loss of mitochondrial outer membrane permeabilization through the inhibition of cleaved caspase-3 activation. RA also inhibited DNA damage, reassuring cell protection. Conclusions: The results suggested a protective effect of RA through downregulation of inflammatory cytokines and cleaved caspase-3.

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“…The chemical components of Spica Prunellae contain various bioactive compounds including triterpenes, flavonoids, phenolic compounds and carbohydrates (5)(6)(7)(8). Among these, oleanolic acid is associated with anticancer (9-11) and anti-inflammatory (12) properties, ursolic acid has shown anti-allergic and anti-inflammatory activities (11,13), rosmarinic acid has demonstrated antioxidant (14,15), neuroprotective (16,17) and anti-inflammatory effects (18), and polysaccharide has anticancer activity (19). As a whole, Spica Prunellae has also been reported to exhibit immunosuppressive activity (20), anti-angiogenic activity (21), vascular inflammation properties (22), anti-estrogenic activity (23), anti-HIV activity (24,25), and anticancer activity (19,26).…”

Section: Introductionmentioning

confidence: 99%

Spica Prunellae extract suppresses the growth of human colon carcinoma cells by targeting multiple oncogenes via activating miR-34a

et al. 2017

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Spica Prunellae is the spike of the herb Prunella vulgaris L. in traditional Chinese medicine which is often used for the treatment of various cancers including colorectal cancer. In the present study, we found that a key tumor suppressor, microRNA-34a (miR-34a) is involved in the antitumor activity for Spica Prunellae. Human colon carcinoma HCT-8 cells treated with an ethanol extract of Spica Prunellae (EESP) had significantly decreased cell proliferation and viability, in a dose-dependent manner. Flow cytometry analysis with Annexin V/PI staining analysis revealed that EESP treatment could induce apoptosis of HCT-8 cells. The level of miR-34a was upregulated in HCT-8 cells following EESP treatment, whereas expression levels of its target genes Notch1, Notch2 and Bcl-2 were downregulated. Inhibition of miR-34a rescued the expression of these target genes. These results revealed that Spica Prunellae can suppress the growth of HCT-8 cells by targeting Notch1, Notch2 and Bcl-2 via activation of miR-34a.

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Rosmarinic acid exerts a neuroprotective effect in the kainate rat model of temporal lobe epilepsy: Underlying mechanisms

Abstract: RA could attenuate seizure, mitigates oxidative stress, augments the activity of defensive systems, and prevent hippocampal neuronal loss and MFS in the kainate model of TLE.

Cited by 64 publications

References 39 publications

Huperzine A ameliorates cognitive dysfunction and neuroinflammation in kainic acid‐induced epileptic rats by antioxidant activity and NLRP3/caspase‐1 pathway inhibition

Huperzine A ameliorates cognitive dysfunction and neuroinflammation in kainic acid‐induced epileptic rats by antioxidant activity and NLRP3/caspase‐1 pathway inhibition

Rosmarinic Acid Attenuates the Activation of Murine Microglial N9 Cells through the Downregulation of Inflammatory Cytokines and Cleaved Caspase-3

Spica Prunellae extract suppresses the growth of human colon carcinoma cells by targeting multiple oncogenes via activating miR-34a

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