Rosmarinic acid-induced apoptosis and cell cycle arrest in triple-negative breast cancer cells

Messeha, Samia S.; Zarmouh, Najla O.; Asiri, Abrar; Soliman, Karam F.A.

doi:10.1016/j.ejphar.2020.173419

Cited by 62 publications

(61 citation statements)

References 65 publications

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“…These results indicate that RA-SFNs can be regarded as a potential drug targeting cervical and breast cancer. Previous studies have also found that RA induced apoptosis in several cancer cell lines [ 10 , 46 ].…”

Section: Resultsmentioning

confidence: 85%

“…Several in vitro studies with RA found in the literature have shown the ability of RA to inhibit cell proliferation and induce cell cycle arrest in various cancer cells [ 10 , 11 ]. The apoptosis phase after treatment with RA-SFNs was highly evaluated in the following experiment for both cell lines.…”

Section: Resultsmentioning

confidence: 99%

“…Rosmarinic acid (RA), an ester of caffeic acid, is a naturally occurring polyphenolic compound that is found especially in medicinal herbs obtained mainly from the Lamiaceae family, such as Rosmarinus officinalis (rosemary), from which it was formerly isolated [ 1 , 2 ]. RA has been used in Traditional Chinese Medicine since ancient times because of its potent curative activity as a neuroprotective [ 3 ], antioxidant [ 4 ], anti-inflammatory [ 5 ], antimicrobial [ 6 ], anti-allergic [ 7 ], antimutagenic [ 8 ], antivirus [ 9 ] and anticarcinogenic [ 1 , 2 , 10 , 11 ] agent. In tumor research, RA has been recognized as an efficient candidate to suppress several cancer cells through different ways by intervening in tumor cell proliferation, reducing inflammation, inducing apoptosis and interfering with the signaling pathways involved in the up-regulation of metastasis, such as ERK [ 2 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Antitumor Activity of Rosmarinic Acid-Loaded Silk Fibroin Nanoparticles on HeLa and MCF-7 Cells

et al. 2021

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Rosmarinic acid (RA), one of the most important polyphenol-based antioxidants, has drawn increasing attention because of its remarkable bioactive properties, including anti-inflammatory, anticancer and antibacterial activities. The aim of this study was to synthesize and characterize RA-loaded silk fibroin nanoparticles (RA-SFNs) in terms of their physical–chemical features and composition, and to investigate their antitumor activity against human cervical carcinoma and breast cancer cell lines (HeLa and MCF-7). Compared with the free form, RA bioavailability was enhanced when the drug was adsorbed onto the surface of the silk fibroin nanoparticles (SFNs). The resulting particle diameter was 255 nm, with a polydispersity index of 0.187, and the Z-potential was −17 mV. The drug loading content of the RA-SFNs was 9.4 wt.%. Evaluation of the in vitro drug release of RA from RA-SFNs pointed to a rapid release in physiological conditions (50% of the total drug content was released in 0.5 h). Unloaded SFNs exhibited good biocompatibility, with no significant cytotoxicity observed during the first 48 h against HeLa and MCF-7 cancer cells. In contrast, cell death increased in a concentration-dependent manner after treatment with RA-SFNs, reaching an IC50 value of 1.568 and 1.377 mg/mL on HeLa and MCF-7, respectively. For both cell lines, the IC50 of free RA was higher. The cellular uptake of the nanoparticles studied was increased when RA was loaded on them. The cell cycle and apoptosis studies revealed that RA-SFNs inhibit cell proliferation and induce apoptosis on HeLa and MCF-7 cell lines. It is concluded, therefore, that the RA delivery platform based on SFNs improves the antitumor potential of RA in the case of the above cancers.

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Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antitumor Activity of Rosmarinic Acid-Loaded Silk Fibroin Nanoparticles on HeLa and MCF-7 Cells

et al. 2021

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“…Earlier study has shown that limonene induces apoptosis by inhibiting PI3K/Akt pathway [ 24 ]. Moreover, rosmarinic acid was detected in high concentration in different extracts of H. orientalis L. It was reported that rosmarinic acid has the ability to promote cell arrest in the G₀/G₁ phase and modulate the expression of many apoptosis-involved genes [ 25 ]. Additionally, trans-ferulic acid was found at various percentages in four different extracts of H. orientalis L. This compound has shown antiproliferative activity as well as induced the expression of pro-apoptotic factor Bax, and reduced the expression of pro-survival factor survivin [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory and Anticancer Activities of Hyacinthus orientalis L.: An In Vitro and In Vivo Study

Kury

Taha

Talib

2021

Plants

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Hyacinthus orientalis L. (family Hyacinthaceae) is traditionally used to treat different diseases including cancer. In this study, the anticancer and immunomodulatory effects of this plant were evaluated. Hydroalcoholic extract was prepared, and different solvent fractions were obtained using solvent–solvent extraction. In the anticancer part, MTT assay and caspase-3 ELISA kits were used to measure the antiproliferative and apoptosis induction ability for each extract, respectively. In the immunomodulatory part, lymphocyte proliferation assay and cytokines detection kit were used to measure the effect of extracts of acquired immunity. Phagocytosis and pinocytosis induction were used to evaluate the effect of extracts on the innate immunity. GC–MS, LC–MS, and Foline–Ciocalteu assays were used to identify the chemical composition of the plant. Balb/C mice were inoculated with breast cancer and treated with hydroalcoholic extract of H. orientalis L. Results showed that hydroalcoholic extract and n-hexane fraction were highly effective in apoptosis induction. Both extract and fraction were also effective in stimulating lymphocytes proliferation and phagocytosis. Significant reduction in tumor size was achieved after treating tumor-bearing mice with hydroalcoholic extract. Additionally, high cure percentages (50%) were obtained in treated mice. Results of this study showed that H. orientalis L. has promising anticancer and immunomodulatory activities. However, further studies are needed to explore more details of apoptosis induction ability and other mechanisms of action and to measure different signaling pathways responsible for the anticancer and immunomodulatory response.

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“…Luo et al [ 60 ] reported that RA inhibited the proliferation and negatively affected the migratory potential of human oral cancer cells (cell line SCC-15) with a dose-dependent effect. Messeha et al [ 61 ] reported that RA caused significant cytotoxic and antiproliferative effects in two racially different triple-negative breast cancer (TNBC) cell lines in a dose- and time-dependent manner.…”

Section: Biochemical and Health Properties Of The Examined Phenolic Acidsmentioning

confidence: 99%

A Review of the Health Protective Effects of Phenolic Acids against a Range of Severe Pathologic Conditions (Including Coronavirus-Based Infections)

Kiokias

Oreopoulou

2021

Molecules

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Phenolic acids comprise a class of phytochemical compounds that can be extracted from various plant sources and are well known for their antioxidant and anti-inflammatory properties. A few of the most common naturally occurring phenolic acids (i.e., caffeic, carnosic, ferulic, gallic, p-coumaric, rosmarinic, vanillic) have been identified as ingredients of edible botanicals (thyme, oregano, rosemary, sage, mint, etc.). Over the last decade, clinical research has focused on a number of in vitro (in human cells) and in vivo (animal) studies aimed at exploring the health protective effects of phenolic acids against the most severe human diseases. In this review paper, the authors first report on the main structural features of phenolic acids, their most important natural sources and their extraction techniques. Subsequently, the main target of this analysis is to provide an overview of the most recent clinical studies on phenolic acids that investigate their health effects against a range of severe pathologic conditions (e.g., cancer, cardiovascular diseases, hepatotoxicity, neurotoxicity, and viral infections—including coronaviruses-based ones).

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Rosmarinic acid-induced apoptosis and cell cycle arrest in triple-negative breast cancer cells

Cited by 62 publications

References 65 publications

Antitumor Activity of Rosmarinic Acid-Loaded Silk Fibroin Nanoparticles on HeLa and MCF-7 Cells

Antitumor Activity of Rosmarinic Acid-Loaded Silk Fibroin Nanoparticles on HeLa and MCF-7 Cells

Immunomodulatory and Anticancer Activities of Hyacinthus orientalis L.: An In Vitro and In Vivo Study

A Review of the Health Protective Effects of Phenolic Acids against a Range of Severe Pathologic Conditions (Including Coronavirus-Based Infections)

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