Rosmarinic acid mediated neuroprotective effects against H2O2-induced neuronal cell damage in N2A cells

Ghaffari, Hadi; Mudili, Venkataramana; Ghassam, Behrouz Jalali; Nayaka, S. Chandra; Angaswamy, Nataraju; Geetha, Nagaraja; Shetty, Shekar

doi:10.1016/j.lfs.2014.07.010

Cited by 96 publications

(52 citation statements)

References 26 publications

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“…Mice treated daily with 20 mg/kg rosmarinic acid for 5 days given intraperitoneally demonstrated reduced infarct size and neurological deficits while markers of neuroprotection such as synaptophysin and BDNF levels were increased (Fonteles et al 2015). Similarly, an in vitro study using an N2A cell model of neurodegeneration, rosmarinic acid was shown to have neuroprotective activities (Ghaffari et al 2014). The cells were treated with several concentrations of rosmarinic acid prior to H 2 O 2 -induced oxidative stress exhibited a dose-dependent protective effects by reducing ROS production and neuronal loss.…”

Section: Major Phytochemicals Of O Basilicum With Antioxidant Potentialmentioning

confidence: 86%

A Brief Review of Potential Neuroprotective Roles of the Culinary Herb Ocimum basilicum

Azmi¹

2018

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Penyakit neurodegeneratif kebiasaannya menjejaskan golongan yang lebih tua dan ia dicirikan oleh kehilangan neuron secara progresif. Tekanan oksidatif sangat dikaitkan dengan neurodegenerasi. Tumbuhan herba yang disasarkan dalam kajian ini, Ocimum basilicum, biasanya digunakan dalam masakan Indochina dan Itali. Kajian farmakologi ke atas O. basilicum telah menunjukkan aktiviti antioksidan yang tinggi dengan beberapa kajian yang menunjukkan tindakan neuroprotektif. Kajian kepustakaan ringkas ini menekankan potensi neuroprotektif O. basilicum dengan membincangkan tindakan antioksidatif menggunakan ekstrak tumbuhan, minyak pati dan sebatian fitokimia tumbuhan ini pada sistem saraf berdasarkan kajian in vitro dan in vivo. Maklumat terkumpul mengenai tindakan neuroprotektif O. basilicum menunjukkan bahawa perlindungan neuron mungkin disebabkan oleh aktiviti antioksidannya dan sebahagian besarnya disebabkan oleh kehadiran sebatian polifenol seperti asid rosmarinik yang telah dikenal pasti sebagai sebatian utamanya. Walaupun mekanisme tindakan antioksidan O. basilicum telah dicadangkan, kajian selanjutnya diperlukan untuk lebih memahami tindakan antioksidan yang membawa kepada peranan neuroprotektif. Berkemungkinan juga tindakan antioksidan O. basilicum diperantara kesan sinergisme campuran pelbagai jenis fitokimia aktif semula jadi di dalam tumbuhan. Oleh itu, sasaran khusus bagi perlindungan neuron melalui tindakan antioksidan memerlukan kajian praklinikal dan klinikal yang lebih lanjut untuk mengkaji potensi terapeutik O. basilicum terutamanya dalam pencegahan penyakit neurodegeneratif.

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Section: Major Phytochemicals Of O Basilicum With Antioxidant Potentialmentioning

confidence: 86%

A Brief Review of Potential Neuroprotective Roles of the Culinary Herb Ocimum basilicum

Azmi¹

2018

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“…Ghaffari with co-workers observed a decrease in H 2 O 2 -induced ROS production in the N2A cells pretreated with RA in the concentration range 1-25 µM. Results were concentration-dependent [39]. The result of the study of Ramanauskiene et al (2016) showed that the amount of ROS in C6 cells depends on the concentration of RA.…”

Section: Intracellular Oxidative Stress Inhibitionmentioning

confidence: 89%

Mentha Rhizomes as an Alternative Source of Natural Antioxidants

et al. 2020

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Unlike its aerial parts, the underground parts of Mentha have so far been studied only marginally. By examining the polyphenolic fingerprint, the antioxidant efficacy and the mutual antioxidant behaviour of mixtures of mint rhizomes, our study presents a modest contribution to addressing this gap. Firstly, we examined the composition of the mint rhizomes: Mentha × piperita cv. ‘Perpeta’ (MPP), M. longifolia (ML), and M. × villosa cv. ‘Snežna’ (MVS). Our LC-MS-DAD analysis revealed the presence of ten compounds belonging to groups of phenolic acids and flavonoids, of which the rosmarinic acid (RA) and lithospermic were most strongly represented. Secondly, we evaluated the antioxidant activity of rhizome infusions by DPPH and ABTS and on NIH/3T3 cell lines by DCFH-DA. Thirdly, we determined, examined, and explained the mutual interactions of rhizome infusions mixtures. While most of the combinations acted additive, synergy was observed in ternary infusion mixtures. The synergic action was also detected in the combination of MPP rhizome infusion and RA in the DCFH-DA test. The combinations of mint rhizomes and rosmarinic acid displayed a high dose-reduction index. This leads to beneficial dose reduction at a given antioxidant effect level in mixtures, compared to the dose of the parts used alone. So far, the pharmaceutical and food industry has not used mint rhizomes in commercial products. Hence, our study draws attention to further applications of the Mentha rhizomes as a valuable alternative source of natural antioxidants.

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“…Part of the beneficial effect of RA could be attributed to its neuroprotective potential (Fallarini et al, 2009). In this regard, RA could protect N2A cells against oxidative damage (Ghaffari et al, 2014). Furthermore, it is a strong protective agent against the 6-hydroxydopamine-induced degeneration of the nigrostriatal dopaminergic system via regulating the ratio of Bcl-2/Bax gene expression, i.e., involved in the apoptotic pathway (Wang et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Rosmarinic Acid Mitigates Apoptosis and nNOS Immunoreactivity but not MAPK and COX-2, Following Intrahippocampal Kainic Acid in the Rat

Khamse

Sadr

Roghani

et al. 2018

Basic Clin. Neurosci. J.

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Kainic Acid (KA) is an ionotropic glutamate receptor agonist. KA can induce neuronal overactivity and excitotoxicity. Rosmarinic Acid (RA) is a natural polyphenolic compound with antioxidant, anti-apoptotic, anti-neurodegenerative, and anti-inflammatory properties. This study aimed to assess the effect of RA on apoptosis, nNOS-positive neurons number, as well as Mitogen-Activated Protein Kinase (MAPK) and Cyclooxygenase-2 (COX-2) immunoreactivity, following intrahippocampal Kainic acid injection in rats. Methods: The study rats were randomly assigned to three groups of sham, KA (KA was injected into the right side of the hippocampus) and KA+RA (a dose of 10 mg/kg/day through a gavage needle for one week before KA injection). Then, histopathological changes, including apoptosis [Terminal Deoxynucleotidyl Transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay], nNOS-positive neurons number, as well as COX-2 and MAPK immunoreactivity were evaluated in the hippocampus. Results: In the RA pretreated group, nNOS-positive neurons and TUNEL-positive cells were significantly reduced compared to the KA group (P<0.05). COX-2and MAPK immunoreactivity demonstrated no significant changes compared to the KA group. They indicated a significant higher reactivity for COX-2 (P<0.01) and MAPK (P<0.005) versus the sham group. Conclusion: RA had neuroprotective effects, compared to KA, through reduced apoptosis and nNOS-positive neurons, but not MAPK and COX-2.

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Rosmarinic acid mediated neuroprotective effects against H2O2-induced neuronal cell damage in N2A cells

Cited by 96 publications

References 26 publications

A Brief Review of Potential Neuroprotective Roles of the Culinary Herb Ocimum basilicum

A Brief Review of Potential Neuroprotective Roles of the Culinary Herb Ocimum basilicum

Mentha Rhizomes as an Alternative Source of Natural Antioxidants

Rosmarinic Acid Mitigates Apoptosis and nNOS Immunoreactivity but not MAPK and COX-2, Following Intrahippocampal Kainic Acid in the Rat

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