Rosmarinic acid prevents post-operative abdominal adhesions in a rat model

Abstract

This study aims to determine the effects of rosmarinic acid which involved the mechanisms to decrease the postoperative peritoneal adhesion formation in rats. Various incisions and removing a 1 × 1 cm piece of peritoneum was used to induce the peritoneal adhesions. Experimental groups were as follows: 1—Sham group. 2—Control group: Peritoneal adhesions were induced and no treatments were performed. 3—Treatment groups: Following inducing peritoneal adhesions, animals received rosmarinic acid with 50 and 70 mg/kg dosage, respectively. Macroscopic examination of adhesions indicated that adhesion bands were reduced in both treatment groups compared to the control group. Moreover, the adhesion score was decreased in both treatment groups on day 14. Inflammation and fibroblast proliferation were both reduced in the treatment groups on day 14. TGF-β1, TNF-α, and VEGF were all evaluated by western blot and immunohistochemistry on days 3 and 14. Treatment groups reduced inflammatory cytokines on days 3 and 14. The treatment group with a 70 mg/kg dosage decreased TGF-β1 and TNF-α levels more than the other treatment group. The administration of rosmarinic acid significantly reduced MDA and increased CAT levels. In conclusion, the rosmarinic acid was effective to reduce the adhesion bands, inflammatory cytokines, angiogenesis, and oxidative stress.

Preparation of Compound Salvia miltiorrhiza–Blumea balsamifera Nanoemulsion Gel and Its Effect on Hypertrophic Scars in the Rabbit Ear Model

“…proved that salvianolic acid B can inhibit fibrosis by regulating the TGF-β1/Smad signaling pathway . Meanwhile, danshensu, rosmarinic acid, and l -borneol show regulatory effects on angiogenesis. , …”

Therapeutic Potential and Mechanisms of Rosmarinic Acid and the Extracts of Lamiaceae Plants for the Treatment of Fibrosis of Various Organs

“…Peritoneal bands connect the peritoneum to different visceral organs and cause abdominal discomfort, infertility in women, intestinal blockage, and other ailments [112]. RA (50 or 70 mg kg −1 ) inhibited the formation of postoperative peritoneal adhesion in male Wistar albino rats [90]. RA decreased the peritoneal adhesion score and was associated with reduced fibroblast proliferation, the expression of TGF-β1 (a fibrotic marker), TNF-α (an inflammation marker), and vascular endothelial growth factor (VEGF) (an angiogenesis marker), and reduced MDA levels (an oxidative stress marker).…”

Construction of a layer-by-layer self-assembled rosemarinic acid delivery system on the surface of CFRPEEK implants for enhanced anti-inflammatory and osseointegration activities

“…RA with two catechol groups in the structure displays significant biological and pharmacological activities, including anti-oxidant, antidepressant, anti-inflammatory, and anti-bacterial properties. [1][2][3][4][5] Due to these excellent properties, RA has attracted widespread attention in the field of biomedicine. Recently, there have been reports on RA promoting osteoblastic differentiation through the canonical Wnt signaling pathway and preventing osteoporosis through inhibiting receptor activator of nuclear factor kappa-B ligand (RANKL)-induced osteoclastic differentiation.…”