Rosmarinus officinalis L. extract ameliorates intestinal inflammation through MAPKs/NF-κB signaling in a murine model of acute experimental colitis

Abstract

We investigated the anti-inflammatory and anti-colitis effects of Rosmarinus officinalis L. extract (RE) by using both in vitro LPS-activated mouse RAW 264.7 macrophages and in vivo dextran sulfate sodium (DSS)-induced experimental murine colitis and suggested the underlying possible mechanisms. Liquid Chromatography-Mass Spectrometry (LC-MS) analysis was performed to identify the major components present in the RE. The clinical signs, biochemistry, immunoblot, ELISA and histology in colon tissues were assessed in order to elucidate the beneficial effect of RE. RE suppressed the LPS-induced pro-inflammatory cytokine production and the expressions of inflammatory proteins in macrophages. Administration of RE (50 and 100 mg kg(-1)) also significantly reduced the severity of DSS-induced murine colitis, as assessed by the clinical symptoms, colon length and histology. RE administration prevented the DSS-induced activation of p38, ERK and JNK MAPKs, attenuated IκBα phosphorylation and subsequent nuclear translocation and DNA binding of NF-κB (p65). RE also suppressed the COX-2 and iNOS expressions, decreased the levels of TNF-α and IL-6 cytokines and the myeloperoxidase activity in the colon tissue. Histological observation revealed that RE administration alleviated mucosal damage and inflammatory cell infiltration induced by DSS in the colon tissue. Hence, RE could be used as a new preventive and therapeutic food ingredient or as a dietary supplement for inflammatory bowel disease.

Evaluation of the Effects of Peritoneal Lavage with Rosmarinus officinalis Extract against the Prevention of Postsurgical-Induced Peritoneal Adhesion

“…Medicherla et al suggested that R. officinalis extract (50 and 100 mg/kg) attenuated COX-2 and inducible nitric oxide synthase expressions, TNF-α and IL-6 levels, and myeloperoxidase activity in dextran sulfate sodium-induced experimental murine colitis. Moreover, they reported that R. officinalis extract decreases the LPS-induced inflammatory cytokine production and inflammatory protein expression in LPS-activated mouse RAW 264.7 macrophages [19]. These studies confirmed our results regarding the anti-inflammatory effects of R. officinalis extract.…”

Protective Effect of Protocatechuic Acid on TNBS-Induced Colitis in Mice Is Associated with Modulation of the SphK/S1P Signaling Pathway

“…TNF-α-induced phosphorylation of AKT is significantly suppresses in HT-29 colonic epithelial cells by 7- O -succinyl macrolactin A in parallel to inhibition of NF-κB translocation [38]. In murine models of DSS Rosmarinus officinalis extract ameliorates intestinal inflammation through MAP kinases/NF-κB signaling [39]. It is also known that expression of the novel adipokine CTRP4 suppresses colitis in mice with a marked downregulation of ERK, AKT and STAT3 [40].…”

Anti‐inflammatory effects of eriocitrin against the dextran sulfate sodium–induced experimental colitis in murine model

“…Clinical evaluation of the severity of the disease was estimated using the procedure by Medicherla et al The disease activity index was estimated according to the observation of score which is allocated for each disease symptoms, including fluctuation of body weight, consistency of defecation and occurrence of bloody stool (fecal bleeding) and the presence of occult blood by a guaiac test.…”