Rosthorin A inhibits non-small cell lung cancer cell growth and metastasis through repressing epithelial-mesenchymal transition via downregulating Slug

Abstract

Lung cancer always ranks first in the number of cancer deaths every year, accounting for 18.4% of total cancer deaths in 2018. Metastasis is the main cause of death in lung cancer patients. The identification of bioactive components of traditional Chinese medicine is very important for the development of novel reagents against non-small cell lung cancer (NSCLC). Rosthorin A has originated from Rabdosia rosthornii (Diels) Hara which excerpts from ‘Chinese materia medica’, and is known to have ‘clear heat phlegm’ properties in the folk. Little is known about the biological functions and mechanisms of Rosthorin A in cancer cells at present. The role of EMT in metastasis of a tumor cell is self-evident. Slug is an important EMT inducer, which is related to the development of lung cancer. Cell growth, clone assay, cell migration, cell invasion, and protein expression, and NSCLC transplanted tumor growth were performed in A549, H1299, and H1975 cells. Rosthorin A significantly inhibited the growth of NSCLC cells, it could prolong the survival of nude mice. Rosthorin A inhibited the migration and invasion of A549, H1299, and H1975 cells. Rosthorin A up-regulated E-cadherin expression level and down-regulated the expression of β-catenin, N-cadherin, vimentin, Slug, and Twist. Rosthorin A could promote the expression of E-cadherin and inhibit the development of EMT by downregulating Slug, to inhibit the development and metastasis of NSCLC cells. In summary, Rosthorin A could be used as a promising candidate for the treatment of NSCLC patients with recurrence and metastasis.

Knockdown of METTL14 suppresses the malignant progression of non‑small cell lung cancer by reducing Twist expression

“…Due to the high mortality rate (accounting for 18.4% of global cancer-associated deaths in 2018), NSCLC remains a serious public health problem worldwide, and the prognosis of most patients remains poor due to tumor metastasis ( 26 ). Accumulating evidence has demonstrated that m6A regulators contribute to cancer progression and metastasis ( 27 ).…”

Network Pharmacology and Experimental Verification to Explore the Potential Mechanism of Yin-Huo-Tang for Lung Adenocarcinoma Recurrence

“…In the theory of traditional Chinese medicine (TCM), LUAD belongs to the category of “lung obstruction”, which can be treated by “nourishing yin and clearing heat”. Various Chinese herbal medicines and decoctions, such as Rabdosia rosthornii (Diels) H. Hara, 14 Tripterygium Hook.f., 15 Ze-Qi-Tang 16 and Fei-Liu-Ping ointment 17 have been reported to inhibit autophagy and apoptosis of tumor cell, angiogenesis and epithelial-to-mesenchymal transition (EMT) outside the tumor. 18 , 19 Thus, TCM has an advantage in suppressing recurrence in lung cancer postoperative patients.…”

Network Pharmacology and Experiment Verification to Explore the Potential Mechanism of Yin-Huo-Tang for Lung Adenocarcinoma Recurrence

“…In the theory of traditional Chinese medicine (TCM), LUAD belongs to the category of "lung obstruction", which can be treated by "nourishing yin and clearing heat". Various Chinese herbal medicines and decoctions, such as Rabdosia rosthornii (Diels) H.Hara [11], Tripterygium Hook.f. [12], Ze-Qi-Tang [13] and Fei-Liu-Ping ointment [14] have been reported to inhibit autophagy and apoptosis of tumor cell, angiogenesis and epithelial to mesenchymal transition (EMT) outside the tumor [15,16].…”