Rotatin is a novel gene required for axial rotation and left–right specification in mouse embryos

Faisst, Anja M.; Álvarez-Bolado, Gonzalo; Treichel, Dieter; Gruß, Peter

doi:10.1016/s0925-4773(02)00003-5

Cited by 55 publications

(54 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In chicken embryos, studies to investigate defects in axial rotation have not been previously reported and, consequently, the mechanisms responsible for this phenomenon are unknown. On the other hand, in the mouse embryo, defects characterized by a lack of rotation occur in the natural "no turning" mutant (25) and in experimentally induced genetic knockout mutants for the Csk (26,27), rotatin (28,29), and furin (30,31) genes. Real-time PCR shows that Csk mRNA expression is down-regulated 30 min after injection (Fig.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Nerve growth factor regulates axial rotation during early stages of chick embryo development

Manca

Capsoni²,

Luzio³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Nerve growth factor (NGF) was discovered because of its neurotrophic actions on sympathetic and sensory neurons in the developing chicken embryo. NGF was subsequently found to influence and regulate the function of many neuronal and non neuronal cells in adult organisms. Little is known, however, about the possible actions of NGF during early embryonic stages. However, mRNAs encoding for NGF and its receptors TrkA and p75 NTR are expressed at very early stages of avian embryo development, before the nervous system is formed. The question, therefore, arises as to what might be the functions of NGF in early chicken embryo development, before its well-established actions on the developing sympathetic and sensory neurons. To investigate possible roles of NGF in the earliest stages of development, stage HH 11-12 chicken embryos were injected with an anti-NGF antibody (mAb αD11) that binds mature NGF with high affinity. Treatment with anti-NGF, but not with a control antibody, led to a dose-dependent inversion of the direction of axial rotation. This effect of altered rotation after anti NGF injection was associated with an increased cell death in somites. Concurrently, a microarray mRNA expression analysis revealed that NGF neutralization affects the expression of genes linked to the regulation of development or cell proliferation. These results reveal a role for NGF in early chicken embryo development and, in particular, in the regulation of somite survival and axial rotation, a crucial developmental process linked to left-right asymmetry specification.neurotrophins | rotatin | csk | furin | proNGF

show abstract

Section: Resultsmentioning

confidence: 99%

“…Absence of axial rotation was observed in mice in which the rotatin gene, highly expressed in neural tube, somites, and notochord, was knocked out (28,29). Although the rotatin gene has been isolated in chicken (GeneID no.…”

Section: Discussionmentioning

confidence: 99%

Nerve growth factor regulates axial rotation during early stages of chick embryo development

Manca

Capsoni²,

Luzio³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Mutations in three other mouse genes that now are known to be required for centriole duplication, Plk4/Sak, Sil/Stil, and Rotatin/Ana-3, were shown previously to cause arrest at midgestation (48)(49)(50)(51)(52). Defects in Hh-dependent patterning were described in Sil/Stil mutant embryos (53), and increased cell death was observed in Plk4 and Rotatin mutants (48,50). We also observed midgestation lethality associated with increased cell death and up-regulation of p53 in mutant embryos homozygous for a gene trap allele of Cep152, which encodes another core protein of the centriole duplication pathway.…”

Section: Discussionmentioning

confidence: 99%

Acentriolar mitosis activates a p53-dependent apoptosis pathway in the mouse embryo

Bazzi

Anderson

2014

Proc. Natl. Acad. Sci. U.S.A.

178

201

View full text Add to dashboard Cite

Significance Centrioles form the core of centrosomes, which organize cilia and interphase and spindle microtubules in animal cells, but centrosome function has not been defined in mammals in vivo. We show that mouse embryos that lack centrioles and centrosomes survive to midgestation, when they lack primary cilia and cilia-dependent signaling. Despite the absence of centrosomes, bipolar spindle formation, chromosome segregation, cell-cycle profile, and DNA damage response are normal in the mutants. Unlike mutants that lack cilia, most cells in acentriolar embryos activate a p53-dependent apoptotic pathway. The data show that mammalian centrioles promote the efficient and rapid assembly of the mitotic spindle and that a short delay in prometaphase activates a checkpoint that leads to p53-dependent cell death in vivo.

show abstract

“…Other (Dufort et al, 1998;Faisst et al, 2002;Hallonet et al, 2002;Izraeli et al, 1999;Melloy et al, 1998). Like mD2LIC -/-embryos, many of these mutants lack midline expression of Foxa2, one consequence of which would be a downregulation of Shh (Filosa et al, 1997).…”

Section: Figmentioning

confidence: 99%

Targeted deletion of the novel cytoplasmic dynein mD2LIC disrupts the embryonic organiser, formation of the body axes and specification of ventral cell fates

Amer¹,

Martinez-Barbera²,

Rodríguez³

et al. 2004

Development

View full text Add to dashboard Cite

Dyneins have been implicated in left-right axis determination during embryonic development and in a variety of human genetic syndromes. In this paper, we study the recently discovered mouse dynein 2 light intermediate chain (mD2LIC), which is believed to be involved in retrograde intraflagella transport and which, like left-right dynein, is expressed in the node of the mouse embryo. Cells of the ventral node of mouse embryos lacking mD2LIC have an altered morphology and lack monocilia,and expression of Foxa2 and Shh in this structure is reduced or completely absent. At later stages, consistent with the absence of nodal cilia, mD2LIC is required for the establishment of the left-right axis and for normal expression of Nodal, and the ventral neural tube fails to express Shh, Foxa2 and Ebaf. mD2LIC also functions indirectly in the survival of anterior definitive endoderm and in the maintenance of the anterior neural ridge, probably through maintenance of Foxa2/Hnf3β expression. Together, our results indicate that mD2LIC is required to maintain or establish ventral cell fates and for correct signalling by the organiser and midline, and they identify the first embryonic function of a vertebrate cytoplasmic dynein.

show abstract

Rotatin is a novel gene required for axial rotation and left–right specification in mouse embryos

Cited by 55 publications

References 68 publications

Nerve growth factor regulates axial rotation during early stages of chick embryo development

Nerve growth factor regulates axial rotation during early stages of chick embryo development

Acentriolar mitosis activates a p53-dependent apoptosis pathway in the mouse embryo

Targeted deletion of the novel cytoplasmic dynein mD2LIC disrupts the embryonic organiser, formation of the body axes and specification of ventral cell fates

Contact Info

Product

Resources

About