The recent observation of the shadow of the supermassive black hole M87*, located at the centre of the M87 galaxy, by the Event Horizon Telescope collaboration has opened up a new window to probe the strong gravity regime. In this paper, we explicitly demonstrate the consequences of this observation on brane world black hole, whose characteristic feature being existence of a negative tidal charge. Our results are based on three observables associated with the shadow of M87*, namely, deviation from circularity, axis ratio and angular diameter of the shadow. These explicitly demonstrate that the existence of a negative tidal charge parameter, marking a deviation from general relativity, is more favoured.