The rotating batch dialyzer, an exceptionally simple cell for measuring membrane permeabilities, has been characterized in terms of boundary layer permeabilities for six solutes diffusing through Cuprophane. An empirical expression was obtained for calculating boundary layer permeabilities from known values for the rotational speed of the cell, the density and viscosity of the solutions, and the diffusion coefficient of the solute in solution. Permeabilities determined with the rotating cell were confirmed for three solutes by comparing results from an entirely different type of cell, the flat plate dialyzer.