Rotating orthogonal polarization imaging

Morgan, Stephen P.; Zhu, Qingsong; Stockford, Ian M.; Crowe, John A.

doi:10.1364/ol.33.001503

Cited by 21 publications

(8 citation statements)

References 12 publications

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“…The parallel detection captures photons with its original polarization state from both superficial and deeper tissue. The perpendicular detection removes the specular reflection and photons from superficial layer; thus the signal from deeper tissue can be highlighted as suggested by Morgan et al [26], which is the main difference compared with superficial detection in pSFDI. The polarization difference imaging is able to perform superficial detection, however, the imaging depth solely depends on the depolarization capability of the sample.…”

Section: Discussionmentioning

confidence: 94%

Polarized light spatial frequency domain imaging for non-destructive quantification of soft tissue fibrous structures

Yang

Lesicko

Sharma

et al. 2015

Biomed. Opt. Express

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The measurement of soft tissue fiber orientation is fundamental to pathophysiology and biomechanical function in a multitude of biomedical applications. However, many existing techniques for quantifying fiber structure rely on transmitted light, limiting general applicability and often requiring tissue processing. Herein, we present a novel wide-field reflectance-based imaging modality, which combines polarized light imaging (PLI) and spatial frequency domain imaging (SFDI) to rapidly quantify preferred fiber orientation on soft collagenous tissues. PLI utilizes the polarization dependent scattering property of fibers to determine preferred fiber orientation; SFDI imaging at high spatial frequency is introduced to reject the highly diffuse photons and to control imaging depth. As a result, photons scattered from the superficial layer of a multi-layered sample are highlighted. Thus, fiber orientation quantification can be achieved for the superficial layer with optical sectioning. We demonstrated on aortic heart valve leaflet that, at spatial frequency of f = 1mm(-1) , the diffuse background can be effectively rejected and the imaging depth can be limited, thus improving quantification accuracy.

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Section: Discussionmentioning

confidence: 94%

Polarized light spatial frequency domain imaging for non-destructive quantification of soft tissue fibrous structures

Yang

Lesicko

Sharma

et al. 2015

Biomed. Opt. Express

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“…These results are free from surface reflections from both the tissue and the components within the system. Images obtained with the copolar system are dominated by surface reflections from the tissue surface and optical components and have been presented elsewhere 15 to demonstrate the improvement in performance achieved using ROPI. To provide an indication of the dynamic range of the system, it is useful to compare the polarization difference values for the highly organized bovine tendon tissue ͓Fig.…”

Section: Methodsmentioning

confidence: 99%

Experimental and theoretical evaluation of rotating orthogonal polarization imaging

et al. 2009

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Rotating orthogonal polarization imaging is a new technique that provides quantitative measurements of the polarization properties of scattering media, such as tissue, which are free from surface reflections. The technique is investigated using both experiments and Monte Carlo simulations of a polarizing target embedded within a scattering medium. The technique is sensitive to the polarization properties of the target up to a depth of 17 mean free paths. Preliminary images of bovine tendon, lamb tendon, chicken breast, and human skin are also demonstrated.

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“…[33][34][35][36][37][38] Polarized light imaging has been used extensively to enhance surface contrast for dermatologic applications. 39 Demarcation of margins of skin cancers, not visible to the naked eye, has been conducted by several researchers, starting with setups focusing on linear depolarization to other systems, [40][41][42][43] utilizing full Stokes vector polarimetry and out-of-plane approaches. [44][45][46] The skin stratum corneum has been shown to be highly scattering hence producing strong depolarization.…”

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confidence: 99%

Optical phantoms for biomedical polarimetry: a review

et al. 2019

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Calibration, quantification, and standardization of the polarimetric instrumentation, as well as interpretation and understanding of the obtained data, require the development and use of well-calibrated phantoms and standards. We reviewed the status of tissue phantoms for a variety of applications in polarimetry; more than 500 papers are considered. We divided the phantoms into five groups according to their origin (biological/nonbiological) and fundamental polarimetric properties of retardation, depolarization, and diattenuation. We found that, while biological media are generally depolarizing, retarding, and diattenuating, only one of all the phantoms reviewed incorporated all these properties, and few considered at least combined retardation and depolarization. Samples derived from biological tissue, such as tendon and muscle, remain extremely popular to quickly ascertain a polarimetric system, but do not provide quantifiable results aside from relative direction of their principal optical axis. Microspheres suspensions are the most utilized phantoms for depolarization, and combined with theoretical models can offer true quantification of depolarization or degree of polarization. There is a real paucity of birefringent phantoms despite the retardance being one of the most interesting parameters measurable with polarization techniques. Therefore, future work should be directed at generating truly reliable and repeatable phantoms for this metric determination. Diattenuating phantoms are rare and application-specific. Given that diattenuation is considered to be low in most biological tissues, the lack of such phantoms is seen as less problematic. The heterogeneity of the phantoms reviewed points to a critical need for standardization in this field. Ultimately, all research groups involved in polarimetric studies and instruments development would benefit from sharing a limited set of standardized polarimetric phantoms, as is done earlier in the round robin investigations in ellipsometry.

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Rotating orthogonal polarization imaging

Cited by 21 publications

References 12 publications

Polarized light spatial frequency domain imaging for non-destructive quantification of soft tissue fibrous structures

Polarized light spatial frequency domain imaging for non-destructive quantification of soft tissue fibrous structures

Experimental and theoretical evaluation of rotating orthogonal polarization imaging

Optical phantoms for biomedical polarimetry: a review

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