Rotation function studies of southern bean mosaic virus at 22 Å resolution

Abstract

Approximately 60 % of the data to a resolution of 22 A has been collected for an R32 form of southern bean mosaic virus. The data were used to compute the rotation function for fivefold, fourfold, threefold, and twofold symmetry axes. An orientation of the icosahedral virus was determined consistent with the observed function. This differed from an earlier prediction based on apparent spikes of an hkO photograph.

Production, purification, crystallization and structure determination ofH-1 Parvovirus

“…3a and 3f1-4 in Fig. 3b) were interpreted as Klug peaks resulting from the superimposition of the self-Pattersons of crystallographically related capsids at a common origin (Johnson et al, 1975). These peaks were not packing peaks resulting from pseudosymmetry at the 'low' resolution (10.0À5.0 A ˚) used to compute the self-rotation functions as they were also observed for 'high' resolution data between 4.0 and 3.0 A ˚(data not shown), for which such packing peaks would be expected to disappear.…”

The crystal and molecular structure of cis-1,5-bis(toluene-p-sulfonyl)-3,7-dihydroxyoctahydro-1,5-diazocine

“…1). An orientation of the particle about the crystallographic twofold axis was previously suggested on the basis of apparent spikes observed on an hkO precession photograph (Johnson et al, 1974). These conclusions were, however, based on inadequate experimental data.…”

“…Rhombohedral type I crystals were obtained with techniques previously described (Johnson et al, 1974). The crystal morphology necessitated the placement of the [hh0] axis along the camera spindle axis.…”