Rotational manipulation of single cells and organisms using acoustic waves

Ahmed, Daniel; Özçelik, Adem; Bojanala, Nagagireesh; Nama, Nitesh; Upadhyay, Awani; Chen, Yuchao; Hanna-Rose, Wendy; Huang, Tony Jun

doi:10.1038/ncomms11085

Cited by 421 publications

(283 citation statements)

References 71 publications

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“…[147][148][149] The acoustic technique has three merits: (a) the capability of manipulating most microparticles, regardless of optical, electrical, magnetic, or shape properties; (b) the ability to manipulate objects with a range of scales, from nanometer to millimeter; and (c) the capability of rapidly manipulating a single particle or groups of particles.…”

Section: E Acoustic Trapsmentioning

confidence: 99%

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

et al. 2017

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Single cell analysis has received increasing attention recently in both academia and clinics, and there is an urgent need for effective upstream cell sample preparation. Two extremely challenging tasks in cell sample preparation-highefficiency cell enrichment and precise single cell capture-have now entered into an era full of exciting technological advances, which are mostly enabled by microfluidics. In this review, we summarize the category of technologies that provide new solutions and creative insights into the two tasks of cell manipulation, with a focus on the latest development in the recent five years by highlighting the representative works. By doing so, we aim both to outline the framework and to showcase example applications of each task. In most cases for cell enrichment, we take circulating tumor cells (CTCs) as the target cells because of their research and clinical importance in cancer. For single cell capture, we review related technologies for many kinds of target cells because the technologies are supposed to be more universal to all cells rather than CTCs. Most of the mentioned technologies can be used for both cell enrichment and precise single cell capture. Each technology has its own advantages and specific challenges, which provide opportunities for researchers in their own area. Overall, these technologies have shown great promise and now evolve into real clinical applications. Published by AIP Publishing. [http://dx

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Section: E Acoustic Trapsmentioning

confidence: 99%

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

et al. 2017

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“…However, the integration of different on‐chip units into an all‐in‐one, fully functional µFACS remains challenging due to their complex designs or incompatibility for integration. Among the various microfluidic techniques for FACS, acoustofluidics (i.e., the fusion of acoustics and microfluidics) is well recognized because of its amenability to integration, miniaturization, and biocompatibility . For example, Jakobsson et al demonstrated an acoustofluidic FACS that uses bulk acoustic waves (BAW) for both cell focusing and cell deflection, and achieved a throughput of ≈150 events s −1 .…”

Section: Introductionmentioning

confidence: 99%

Standing Surface Acoustic Wave (SSAW)‐Based Fluorescence‐Activated Cell Sorter

Ren

Yang

Zhang

et al. 2018

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Microfluidic fluorescence-activated cell sorters (μFACS) have attracted considerable interest because of their ability to identify and separate cells in inexpensive and biosafe ways. Here a high-performance μFACS is presented by integrating a standing surface acoustic wave (SSAW)-based, 3D cell-focusing unit, an in-plane fluorescent detection unit, and an SSAW-based cell-deflection unit on a single chip. Without using sheath flow or precise flow rate control, the SSAW-based cell-focusing technique can focus cells into a single file at a designated position. The tight focusing of cells enables an in-plane-integrated optical detection system to accurately distinguish individual cells of interest. In the acoustic-based cell-deflection unit, a focused interdigital transducer design is utilized to deflect cells from the focused stream within a minimized area, resulting in a high-throughput sorting ability. Each unit is experimentally characterized, respectively, and the integrated SSAW-based FACS is used to sort mammalian cells (HeLa) at different throughputs. A sorting purity of greater than 90% is achieved at a throughput of 2500 events s . The SSAW-based FACS is efficient, fast, biosafe, biocompatible and has a small footprint, making it a competitive alternative to more expensive, bulkier traditional FACS.

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“…As a noninvasive and noncontact manipulation method, acoustic trapping has the advantage that it is able to simply levitate many kinds of objects from small living animal to bacteria 15, 16, 17, 18, 19, 20, 21. More importantly, the penetration of sound wave is much deeper than light in nontransparent media.…”

Section: Introductionmentioning

confidence: 99%

Observation of Metal Nanoparticles for Acoustic Manipulation

et al. 2017

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Use of acoustic trapping for the manipulation of objects is invaluable to many applications from cellular subdivision to biological assays. Despite remarkable progress in a wide size range, the precise acoustic manipulation of 0D nanoparticles where all the structural dimensions are much smaller than the acoustic wavelength is still present challenges. This study reports on the observation of metal nanoparticles with different nanostructures for acoustic manipulation. Results for the first time exhibit that the hollow nanostructures play more important factor than size in the nanoscale acoustic manipulation. The acoustic levitation and swarm aggregations of the metal nanoparticles can be easily realized at low energy and clinically acceptable acoustic frequency by hollowing their nanostructures. In addition, the behaviors of swarm aggregations can be flexibly regulated by the applied voltage and frequency. This study anticipates that the strategy based on the unique properties of the metal hollow nanostructures and the manipulation method will be highly desirable for many applications.

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Rotational manipulation of single cells and organisms using acoustic waves

Cited by 421 publications

References 71 publications

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

Standing Surface Acoustic Wave (SSAW)‐Based Fluorescence‐Activated Cell Sorter

Observation of Metal Nanoparticles for Acoustic Manipulation

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