Rotavirus and illness severity in children presenting with acute gastroenteritis at the primary care out-of-hours service

Wolters, Pien I; Holtman, Gea A; Weghorst, Anouk Ah; Knoester, Marjolein; Berger, Marjolein Y.

doi:10.1080/13814788.2021.2011205

Cited by 2 publications

(2 citation statements)

References 23 publications

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“…The top three viruses with the highest viral loads were AdV, norovirus GII followed by RVA. In other studies, using C t values, RVA was on the top followed by norovirus and AstV 73 . We did not observe any correlation among titres of single inflection and co‐infection.…”

Section: Discussioncontrasting

confidence: 68%

“…In other studies, using C t values, RVA was on the top followed by norovirus and AstV. 73 We did not observe any correlation among titres of single inflection and co-infection. Establishing biological relevance to viral titre, particularly in the context of co-infection, remains an open question which could be further investigated with a larger number of co-infection cases.…”

Section: 1%contrasting

confidence: 58%

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Ten different viral agents infecting and co‐infecting children with acute gastroenteritis in Southern Italy: Role of known pathogens and emerging viruses during and after COVID‐19 pandemic

Amoroso,

Pucciarelli,

Serra

et al. 2024

Journal of Medical Virology

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Acute gastroenteritis (AGE) represents a world public health relevant problem especially in children. Enteric viruses are the pathogens mainly involved in the episodes of AGE, causing about 70.00% of the cases. Apart from well‐known rotavirus (RVA), adenovirus (AdV) and norovirus (NoV), there are various emerging viral pathogens potentially associated with AGE episodes. In this study, the presence of ten different enteric viruses was investigated in 152 fecal samples collected from children hospitalized for gastroenteritis. Real time PCR results showed that 49.3% of them were positive for viral detection with the following prevalence: norovirus GII 19.7%, AdV 15.8%, RVA 10.5%, human parechovirus (HPeV) 5.3%, enterovirus (EV) 3.3%, sapovirus (SaV) 2.6%. Salivirus (SalV), norovirus GI and astrovirus (AstV) 1.3% each, aichivirus (AiV) found in only one patient. In 38.2% of feces only one virus was detected, while co‐infections were identified in 11.8% of the cases. Among young patients, 105 were ≤5 years old and 56.0% tested positive for viral detection, while 47 were >5 years old with 40.0% of them infected. Results obtained confirm a complex plethora of viruses potentially implicated in gastroenteritis in children, with some of them previously known for other etiologies but detectable in fecal samples. Subsequent studies should investigate the role of these viruses in causing gastroenteritis and explore the possibility that other symptoms may be ascribed to multiple infections.

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Section: Discussioncontrasting

confidence: 68%

Section: 1%contrasting

confidence: 58%

Ten different viral agents infecting and co‐infecting children with acute gastroenteritis in Southern Italy: Role of known pathogens and emerging viruses during and after COVID‐19 pandemic

Amoroso,

Pucciarelli,

Serra

et al. 2024

Journal of Medical Virology

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Evaluation of the diagnostic performance of EpiTuub® Fecal Rotavirus Antigen Rapid Test Kit in Amhara National Regional State, Ethiopia: A multi-center cross-sectional study

Damtie,

Gelaw,

Wondimeneh

et al. 2023

PLoS ONE

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Rotavirus is the leading cause of morbidity and mortality due to acute gastroenteritis among children under five years globally. Early diagnosis of rotavirus infection minimizes its spread and helps to determine the appropriate management of diarrhea. The aim of this study was to evaluate the performance of EpiTuub® Fecal Rotavirus Antigen Rapid Test Kit for the diagnosis of rotavirus infection among diarrheic children under five years in Ethiopian healthcare settings. A total of 537 children with diarrhea were enrolled from three referral hospitals in Amhara National Regional State, Ethiopia. The samples were tested using one-step RT-PCR and EpiTuub® Fecal Rotavirus Antigen Rapid Test Kit (KTR-917, Epitope Diagnostics, San Diego USA) in parallel. Diagnostic performance of the rapid test kit was evaluated using the one-step RT-PCR as a gold standard. The sensitivity, specificity, and predictive values of the rapid test kit were determined. Moreover, the agreement of the rapid test kit with one step RT-PCR was determined by kappa statistics and receiver operators’ curve (ROC) analysis was done to assess the overall diagnostic accuracy of the rapid test kit. Fecal Rotavirus Antigen Rapid Test Kit has shown a sensitivity of 75.5% and specificity of 98.2%. The kit was also found to have 89.9% and 95.0% positive and negative predictive values, respectively. The Fecal Rotavirus Antigen Rapid Test Kit has shown a substantial agreement (78.7%, p = 0.0001) with one-step RT-PCR. The overall accuracy of the Fecal Rotavirus Antigen Rapid Test Kit was excellent with the area under the ROC curve of 86.9% (95% CI = 81.6, 92.1%) (p = .0001). Thus, Fecal Rotavirus Antigen Rapid Test is a sensitive, specific, user-friendly, rapid, and equipment-free option to be used at points of care in Ethiopian health care settings where resource is limited precluding the use of one step RT-PCR. Furthermore, the kit could be used in the evaluation and monitoring of rotavirus vaccine effectiveness in the aforementioned settings.

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Rotavirus and illness severity in children presenting with acute gastroenteritis at the primary care out-of-hours service

Cited by 2 publications

References 23 publications

Ten different viral agents infecting and co‐infecting children with acute gastroenteritis in Southern Italy: Role of known pathogens and emerging viruses during and after COVID‐19 pandemic

Ten different viral agents infecting and co‐infecting children with acute gastroenteritis in Southern Italy: Role of known pathogens and emerging viruses during and after COVID‐19 pandemic

Evaluation of the diagnostic performance of EpiTuub® Fecal Rotavirus Antigen Rapid Test Kit in Amhara National Regional State, Ethiopia: A multi-center cross-sectional study

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