Rotavirus genotypes in children with gastroenteritis in Erzurum: first detection of G12P[6] and G12P[8] genotypes in Turkey

Abstract

IntroductionRotavirus is one of the leading pathogens which cause acute gastroenteritis in children and is responsible for a substantial proportion of childhood deaths worldwide.AimTo determine the group A rotavirus (RVA) prevalence and genotypes of circulating RVA strains in 0–5-year-old children with complaints of vomiting and diarrhoea in Eastern Anatolia in Turkey.Material and methodsRNA extracted from stool specimens of 329 children aged 0–5 years with acute diarrhoea was subjected to reverse transcription polymerase reaction (RT-PCR) and multiplex-nested PCR. The genotypes were identified based on the expected size of the amplicon, which was amplified with a genotype-specific primer.ResultsOut of 329 stool samples analyzed, 109 (33.1%) were positive for RVA. G1P[8] was the dominant genotype combination (42.2%), followed by G9P[8] (21.1%) and G12P[6] (11.0%). Mixed infections were identified in 5 cases: G3,9 in 2 cases, G1,9 in 1 case, P[4,8] in 1 case, and P[6,8] in 1 case. The P genotype could not be typed in two patients.ConclusionsIn the study, we detected six different rotavirus G genotypes, 3 different P genotypes, 11 different G-P combinations and 5 different mixed genotypes combinations. G1, G9, G12 and P[8] were found to be the predominant genotypes. G12P[6] and G12P[8] genotypes, showing an increase as new rotavirus genotypes in the world, are reported for the first time for our regions. We determined the dominant genotypes, mixed genotypes and unconventional genotypes of rotavirus in our region.

Epidemiologic characteristics and clinical course of children with acute gastroenteritis hospitalized in the Department of Pediatric Infectious Diseases and Hepatology at John Paul II Hospital in Krakow

“…Rotavirus is the leading cause of AGE in infants and young children and the major contributor to hospitalization for diarrhea in countries that have no rotavirus vaccines in their national immunization schedules (3,4,5). With the continuing decline in cases of rotavirus-associated AGE, since the implementation of routine childhood vaccination against rotavirus, norovirus infection has become the most common cause of medically treated AGE (6,7).…”

“…W krajach niemających w obowiązku szczepień przeciwko rotawirusom, wirus ten jest najczęstszą przyczyną AGE u niemowląt i małych dzieci oraz głównym powodem hospitalizacji dzieci z biegunką (3,4,5). W państwach, gdzie szczepienia przeciwko rotawirusom zostały włączone do narodowego programu szczepień, zaobserwowano zmniejszenie liczby hospitalizacji dzieci z powodu infekcji rotawirusowej oraz znaczne zwiększenie udziału norowirusa w etiologii AGE (6,7).…”

“…WYNIKI. Mediana wieku chorych wynosiła 3,0 roku [1,5]. 80% wszystkich przypadków AGE wystąpiło u dzieci < 5 r.ż.…”

Buzağı İshallerinde Coronavirusun Nukleoprotein Gen ve Rotavirusun VP7/VP4 Gen Bölgelerinin Kısmi Sekansı ve Filogenetik Analizi

“…Rotavirusların dış kapsid proteini olan VP4 (viral protein 4) ve VP7 (viral protein 7), konakta nötralizan antikor üretimini tetiklemekle beraber, bu proteinleri kodlayan nükleotid sekansına göre virusun P (proteaz) ve G (glikoprotein) genotipleri belirlenebilmektedir. Şimdiye kadar rotavirusun bilinen en az 27 farklı G ve 37 farklı P genotipi tespit edilmiştir (1,(3)(4)(5). Diğer bir enterik patojen olan coronaviruslar genetik yakınlıklarına göre; Alfacoronavirus, Betacoronavirus, Gamacoronavirus ve Deltacoronavirus olmak üzere dört gruba ayrılmaktadır (6).…”

Molecular characterization of group A rotavirus from rhesus macaques ( Macaca mulatta ) at human–wildlife interfaces in Bangladesh

“…The majority of ongoing research on rotaviruses (RVs) is focused on the reassortment of VP7 and VP4. Different combinations of G and P genotypes have been detected in humans (Aydin & Aktaş, 2017;Saudy, Elshabrawy, Megahed, Foad, & Mohamed, 2016). The most common G types, which are found globally in humans are G1, G2, G3 and G4 in conjunction with P[8] or P[4], followed by G9 associated with P[8] or P [6].…”