2011
DOI: 10.1590/s1413-86702011000300006
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Rotavirus infection in a tertiary hospital: laboratory diagnosis and impact of immunization on pediatric hospitalization

Abstract: Background and objectives: Rotavirus (RV) is the main etiological agent of diarrhea in childhood; its laboratory diagnosis is crucial to guide the clinical management and prevention of its spread. RV immunization was introduced in Brazilian 6-month-old children in 2006. The present study was aimed to evaluate three methodologies used for human RV detection in stool samples obtained from patients hospitalized due to gastroenteritis in a teaching hospital and report the impact of RV immunization in hospitalizati… Show more

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Cited by 2 publications
(1 citation statement)
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“…42 The fecal rotavirus antigen is examined through ELISA and latex agglutination test; search of adenovirus fecal antigen is performed using ELISA. 98 Polymerase chain reaction (PCR) or other molecular investigations are performed for viral research, especially on stools for norovirus, adenovirus, sapovirus, and human bocavirus, and PCR of stool and blood is used for the detection of enteroviruses. 63 A Providencia genus-specific PCR method has been developed, and its specificity and sensitivity was evaluated to be 100% with various bacterial strains.…”
Section: Laboratory Diagnosismentioning
confidence: 99%
“…42 The fecal rotavirus antigen is examined through ELISA and latex agglutination test; search of adenovirus fecal antigen is performed using ELISA. 98 Polymerase chain reaction (PCR) or other molecular investigations are performed for viral research, especially on stools for norovirus, adenovirus, sapovirus, and human bocavirus, and PCR of stool and blood is used for the detection of enteroviruses. 63 A Providencia genus-specific PCR method has been developed, and its specificity and sensitivity was evaluated to be 100% with various bacterial strains.…”
Section: Laboratory Diagnosismentioning
confidence: 99%