Rotavirus reverse genetics: A tool for understanding virus biology

Papa, Guido; Burrone, Óscar R.

doi:10.1016/j.virusres.2021.198576

Cited by 7 publications

(4 citation statements)

References 56 publications

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“…59 Subsequently, Himetobi P virus (Hipve) 59,61 and Nilla-parvata lugens symbiont X virus (NLCXV) 62 were found in planthopper populations. With the rapid development of HTS [64][65][66] In sustainable agricultural pest management, it offers significant potential for modifying and controlling insect viruses. Despite the development of many reverse genetics systems for arthropod-borne viruses, their widespread agricultural application has been limited by the lack of suitable insect cell lines.…”

Section: Planthoppermentioning

confidence: 99%

“…Reverse genetics technology, a rapidly growing field in virology, deciphers complex viral genome structures and functions. It allows viral genome manipulation in vitro , analysis of recombinant viruses, and virus vector modification for purposes like foreign protein expression, gene silencing, and vaccine development 64–66 . In sustainable agricultural pest management, it offers significant potential for modifying and controlling insect viruses.…”

Section: Reverse Genetics Technology For Agricultural Insect Virusesmentioning

confidence: 99%

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Leveraging insect viruses and genetic manipulation for sustainable agricultural pest control

Sun,

Fu,

et al. 2023

Pest Management Science

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Insect viruses have long been recognized for their potential as biological control agents in managing agricultural pests. Despite their promise, challenges such as host specificity, virulence, and limited viral resources have hindered their widespread application. The need for efficient discovery and utilization of insect viruses is paramount for sustainable agricultural practices. With the introduction of high‐throughput sequencing (HTS) technologies, the discovery and identification of new insect viruses have been significantly accelerated, enriching the available resources for pest management. Furthermore, advancements in reverse genetics tools have enabled the development of multifunctional viral expression vectors, enhancing the specificity and efficacy of insect viruses against targeted pests. This review provides a comprehensive overview of the methodologies for insect virus identification using HTS and the potential of genetically modified insect viruses in pest control. Genetically engineered insect viruses offer a promising avenue for targeted and efficient pest management. While HTS technologies have revolutionized the discovery of insect viruses, challenges remain. Addressing these challenges and harnessing the potential of these technologies will be pivotal in shaping sustainable agricultural management strategies in the future.This article is protected by copyright. All rights reserved.

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Section: Planthoppermentioning

confidence: 99%

Section: Reverse Genetics Technology For Agricultural Insect Virusesmentioning

confidence: 99%

Leveraging insect viruses and genetic manipulation for sustainable agricultural pest control

Sun,

Fu,

et al. 2023

Pest Management Science

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“…The RG system developed by Sánchez-Tacuba et al, which capitalizes on a plasmid encoding a fusion protein consisting of T7 polymerase and the African swine fever virus NP868R capping enzyme, as well as an IFN-insensitive MA104 cell line, has been used to recover hard-to-culture RV strains, including simian RRV and human CDC-9; a recombinant RRV expressing EGFP; a murine RV reassortment D6/2-like virus ( 104 ); a murine RV lacking NSP1 expression ( 112 ); and a murine RV encoding a Nano-Luciferase bioluminescent reporter ( 113 ). Extensive reviews of the current plasmid-only RG systems are available elsewhere ( 7 , 114 – 116 ).…”

Section: Rv Studies In the Era Of Reverse Geneticsmentioning

confidence: 99%

“…(a) RNA sensing and antagonism of type I and III IFN signaling. Upon entry into the cell, RLR-sensing of RV dsRNA activates MAVS ( 116 , 117 ). Previous studies have demonstrated that RIG-I and MDA5 are independently essential for antiviral responses to RV in vitro , suggesting that RV generates RNA species recognized by both receptors, which are degraded by NSP1 ( 12 , 118 , 119 ).…”

Section: Rv Studies In the Era Of Reverse Geneticsmentioning

confidence: 99%

Re-Examining Rotavirus Innate Immune Evasion: Potential Applications of the Reverse Genetics System

Antia

Pinski

Ding

2022

mBio

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Rotaviruses represent one of the most successful pathogens in the world, with high infectivity and efficient transmission between the young of many animal species, including humans. To overcome host defenses, rotaviruses have evolved a plethora of strategies to effectively evade the innate immune response, establish initial infection in the small intestine, produce progeny, and shed into the environment. Previously, studying the roles and relative contributions of specific rotaviral factors in innate immune evasion had been challenging without a plasmid-only reverse genetics system.

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Rescue of bovine ephemeral fever virus through reverse genetics, but inability to propagate

Jasmeen,

Gupta,

Kaur

et al. 2024

VirusDis.

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Rotavirus reverse genetics: A tool for understanding virus biology

Cited by 7 publications

References 56 publications

Leveraging insect viruses and genetic manipulation for sustainable agricultural pest control

Leveraging insect viruses and genetic manipulation for sustainable agricultural pest control

Re-Examining Rotavirus Innate Immune Evasion: Potential Applications of the Reverse Genetics System

Rescue of bovine ephemeral fever virus through reverse genetics, but inability to propagate

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