Rotigotine protects against oxidized low-density lipoprotein(ox-LDL)-induced damages in human umbilical vein endothelial cells(HUVECs)

Kang, Hui; Yu, Hui; Fan, Jingxiu; Cao, Ge

doi:10.1080/21655979.2021.2000224

Cited by 13 publications

(3 citation statements)

References 49 publications

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“…Moreover, when atherosclerotic injury occurs, endothelial cells release adhesion factors and attract monocytes to accumulate, which can reflect cell adhesion (35), and U937 is a human monocyte. U937 cells were used to detect the changes of HUVEC adhesion ability in the present study (36). It was found that NEDD4 overexpression led to a decrease in the levels of ROS, iNOS, VCAM-1, ICAM-2 and the number of U937-HUVEC adhesion cells, but an increase in the level of eNOS.…”

Section: Discussionmentioning

confidence: 71%

NEDD4 attenuates oxidized low‑density lipoprotein‑induced inflammation and dysfunction in vascular endothelial cells via regulating APEX1 expression

Tan

et al. 2023

Exp Ther Med

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Atherosclerosis chiefly results from inflammation as well as vascular endothelial cell dysfunction. Methylation levels of neuronally expressed developmentally downregulated 4 (NEDD4) were found to be fortified in atherosclerosis patients and NEDD4 deficiency enhanced vascular calcification. However, the exact function of NEDD4 in inflammation and vascular endothelial dysfunction remains to be elucidated. In the present study, CCK-8 assay was used to estimate cell viability. Reverse transcription-quantitative PCR was adopted to examine the expression of NEDD4, inflammation-associated enzymes and apurinic/apyrimidinic endodeoxyribonuclease 1 (APEX1). Western blotting was used to test NEDD4, endothelial nitric oxide synthase, inducible nitric oxide synthase and APEX1 protein levels. Cytotoxicity was detected by a lactate dehydrogenase (LDH) kit. Reactive oxygen species level was tested by a corresponding kit. Vascular cell adhesion molecule 1 and intercellular adhesion molecule 1 contents were examined with ELISA. Cell adhesion assays evaluated the adhesion of endothelial cells. Co-immunoprecipitation assay was used to test the relationship between NEDD4 and APEX1. The data revealed that NEDD4 expression rapidly declined in oxidized low density lipoprotein (ox-LDL)-induced human umbilical vein endothelial cells (HUVECs). Following NEDD4 overexpression, the active damage, inflammatory release and endothelial cell dysfunction in ox-LDL-induced HUVECs were attenuated. After co-transfection of APEX1 interference plasmids and NEDD4 overexpression plasmids, cell damage, inflammatory release and endothelial cell dysfunction in ox-LDL-induced HUVECs were improved again. Taken together, NEDD4 attenuated ox-LDL-induced inflammation and endothelial dysfunction by regulating APEX1 expression.

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Section: Discussionmentioning

confidence: 71%

NEDD4 attenuates oxidized low‑density lipoprotein‑induced inflammation and dysfunction in vascular endothelial cells via regulating APEX1 expression

Tan

et al. 2023

Exp Ther Med

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“…It was previously shown that the reduction of PLMS with rotigotine was significantly associated with a decrease in blood pressure (Bauer et al, 2016); however, lipid values were not measured. Of note, rotigotine attenuated cholesterol uptake in endothelial cells thus having an anti‐atherosclerotic effect (Kang et al, 2021). We hypothesised that increased MSNA is the reason for dyslipidaemia in PLMS.…”

Section: Discussionmentioning

confidence: 99%

The relationship between periodic limb movement during sleep and dyslipidaemia in patients with obstructive sleep apnea

Bikov,

Bailly,

Testelmans

et al. 2023

Journal of Sleep Research

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SummaryPeriodic limb movements during sleep and obstructive sleep apnea are both associated with increased sympathetic tone, and have been proposed as risk factors for heart diseases and, in particular, cardiovascular disease. As sympathetic system activation may lead to dyslipidaemia, periodic limb movements during sleep could be an additional risk factor for cardiovascular disease in patients with obstructive sleep apnea. The aim of the study was to determine whether the presence of periodic limb movements during sleep affects serum lipid levels in obstructive sleep apnea. Total cholesterol, low‐density lipoprotein cholesterol, high‐density lipoprotein cholesterol, non‐ high‐density lipoprotein cholesterol and triglyceride levels were investigated in 4138 patients with obstructive sleep apnea in the European Sleep Apnea Database (ESADA) cohort, divided into those with periodic limb movements during sleep index ≥ 15 per hr (n = 628) and controls (n = 3510). ANCOVA adjusted for age, sex, body mass index, apnea–hypopnea index, alcohol intake, smoking status, diabetes, insomnia and study site was used to assess differences in lipids between periodic limb movements during sleep and controls. Patients with periodic limb movements during sleep (24% female, 54.4 ± 12.1 years, body mass index 31.9 ± 5.8 kg m−2, apnea–hypopnea index 36.7 ± 25.4 per hr) had higher triglyceride (1.81 ± 1.04 versus 1.69 ± 0.90 mmol L−1, p = 0.002) and lower high‐density lipoprotein cholesterol (1.19 ± 0.34 versus 1.24 ± 0.37 mmol L−1, p = 0.002) levels, whilst there was no difference in either total cholesterol (4.98 ± 1.10 versus 4.94 ± 1.07 mmol L−1), low‐density lipoprotein cholesterol (3.04 ± 0.96 versus 2.98 ± 0.98 mmol L−1) or non‐ high‐density lipoprotein cholesterol (3.78 ± 1.10 versus 3.70 ± 1.05 mmol L−1) concentrations (all p > 0.05). The results remained unchanged after most sensitivity analyses. Patients with obstructive sleep apnea with periodic limb movements during sleep had more prevalent cardiovascular disease (11% versus 6%, p < 0.01). Periodic limb movements during sleep in obstructive sleep apnea is associated with dyslipidaemia independently of important confounders. Our results highlight periodic limb movements during sleep as an additional risk factor for cardiovascular disease in obstructive sleep apnea.

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“…The co-stimulation of both B2R and D2R receptors regulated the expression of markers of apoptosis such as Bcl-2, Bcl-xL, and Bax and inhibited the release of IL-6 and endothelin-1 [ 89 ]. In human umbilical vein endothelial cells, the D2-like receptor agonist rotigotine (D2R > D3R > D4R > D5R > D1R) inhibited NF-κB activation and reduced its reporter activity, exerting anti-inflammatory actions [ 90 ]. DA, receptor not determined, also regulated the interaction of leukocytes and the endothelium, decreasing the transendothelial migration of leukocytes and the expression of ICAM-1 and E-selectin in endothelial cells and attenuating the chemoattractant effect of IL-8 [ 91 , 92 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-Inflammatory Effects of Peripheral Dopamine

Moore,

Vaz de Castro,

Yaqub

et al. 2023

IJMS

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Dopamine is synthesized in the nervous system where it acts as a neurotransmitter. Dopamine is also synthesized in a number of peripheral organs as well as in several types of cells and has organ-specific functions and, as demonstrated more recently, is involved in the regulation of the immune response and inflammatory reaction. In particular, the renal dopaminergic system is very important in the regulation of sodium transport and blood pressure and is particularly sensitive to stimuli that cause oxidative stress and inflammation. This review is focused on how dopamine is synthesized in organs and tissues and the mechanisms by which dopamine and its receptors exert their effects on the inflammatory response.

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Rotigotine protects against oxidized low-density lipoprotein(ox-LDL)-induced damages in human umbilical vein endothelial cells(HUVECs)

Cited by 13 publications

References 49 publications

NEDD4 attenuates oxidized low‑density lipoprotein‑induced inflammation and dysfunction in vascular endothelial cells via regulating APEX1 expression

NEDD4 attenuates oxidized low‑density lipoprotein‑induced inflammation and dysfunction in vascular endothelial cells via regulating APEX1 expression

The relationship between periodic limb movement during sleep and dyslipidaemia in patients with obstructive sleep apnea

Anti-Inflammatory Effects of Peripheral Dopamine

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