This paper presents a trifocal Rotman lens design approach. The effect due to change of substrate on the circular contour is observed. The shape of the beam contour is taken as circular. Different substrates can be used for the fabrication of the lens. Three different materials have been used to fabricate the lens antenna .A three beam prototype feeding five element antenna array working in ISM band has been simulated using RLD1.7.Effects on the performance of the antenna is observed.Index Terms-Rotman lens antenna, RLD, Dielectric constant, substrate, array factor, phase error, side lobe level.