Rough and smooth morphotypes isolated from Lactobacillus farciminis CNCM I-3699 are two closely-related variants

Tareb, Raouf; Bernardeau, Marion; Horvath, Philippe; Vernoux, Jean-Paul

doi:10.1016/j.ijfoodmicro.2014.08.036

Cited by 9 publications

(5 citation statements)

References 63 publications

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“…The Prophinder ( http://aclame.ulb.ac.be/Tools/Prophinder/ ) ( 10 ) and IS finder ( http://www-is.biotoul.fr ) ( 11 ) were used to detect atypical genome regions corresponding to a prophage, putative horizontal gene transfer, insertion sequence (IS). Clustered regularly interspaced short palindromic repeat (CRISPR) elements already reported ( 5 ) were confirmed by the CRISPR Finder web software ( 12 ). The specific plasmid of the smooth variant comprises a capsular polysaccharide locus that was flanked by phage sequences.…”

Section: Genome Announcementmentioning

confidence: 54%

“…The probiotic properties of this strain were demonstrated in vitro ( 3 , 4 ) and in vivo ( 2 ). Interestingly, L. farciminis CNCM-I-3699 is a pleomorphic strain reproducibly giving rise to two phenotypically distinct morphotypes R for rough ( L. farciminis CNCM-I-3699-R) and S for smooth ( L. farciminis CNCM-I-3699-S) ( 5 ). Significant differences in capsular polysaccharide production were observed: the smooth phenotype corresponds to circular colony with slimy filamentous aspect “ropy”; while the rough morphotype gives irregular, flat, and nonropy colonies.…”

Section: Genome Announcementmentioning

confidence: 99%

“…The specific plasmid of the smooth variant comprises a capsular polysaccharide locus that was flanked by phage sequences. In addition, it includes a sugar transporter and genes related to growth in anaerobic conditions, potentially explaining the predominance of the S variant in anaerobic conditions ( 5 ). A more comprehensive report, comparing the complete genomes of the two variants in relation to probiotic properties, genome plasticity, and probiotic lifestyle, will be the subject of a future publication.…”

Section: Genome Announcementmentioning

confidence: 99%

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Genome Sequence of Rough and Smooth Variants of Pleomorphic Strain Lactobacillus farciminis CNCM-I-3699

2015

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Section: Genome Announcementmentioning

confidence: 54%

Section: Genome Announcementmentioning

confidence: 99%

Section: Genome Announcementmentioning

confidence: 99%

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Genome Sequence of Rough and Smooth Variants of Pleomorphic Strain Lactobacillus farciminis CNCM-I-3699

2015

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“…The smooth morphotype, ropy on plating, was shown to produce greater quantities of capsular PS, compared to the rough one. The rough morphotype was shown to aggregate and sediment, whereas the smooth one did not (Tareb et al, 2015(Tareb et al, , 2017. In Lactobacillus johnsonii, the strain FI9785 and its spontaneous variant presented different colony morphotypes with different surface properties that were linked to the production of a PS synthesis (Horn et al, 2013).…”

Section: Pf 456: a Derivative That Has Irreversibly Lost The Molar-shmentioning

confidence: 99%

Surface properties associated with the production of polysaccharides in the food bacteria Propionibacterium freudenreichii

et al. 2020

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This study explores the production of polysaccharides (PS) in the strain Pf2289 of the food species Propionibacterium freudenreichii. Pf2289 presents characteristics atypical of the species: a molar-shaped morphotype upon plating, and cells strongly aggregative in liquid medium. When plating Pf2289, another morphotype was observed with a 4% frequency of appearance: round-shaped colonies, typical of the species. A clone was isolated, designated Pf456. No reversibility of Pf456 towards the molar-shaped morphotype was observed. Pf2289 was shown to produce a surface polysaccharide (PS) bound to the cell wall, mainly during the stationary growth phase. Meanwhile, Pf456 had lost the ability to produce the PS. AFM images of Pf2289 showed that entangled filaments spread over the whole surface of the bacteria, whereas Pf456 exhibited a smooth surface. Adhesion force maps, performed with concanavalin-A grafted probes, revealed twice as much adhesion of Pf2289 to concanavalin-A compared to Pf456. Furthermore, the length of PS molecules surrounding Pf2289 measured at least 7 μm, whereas it only reached 1 μm in Pf456. Finally, the presence of PS had a strong impact on adhesion properties: Pf2289 did not adhere to hydrophobic surfaces, whereas Pf456 showed strong adhesion.

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“…The influence of L. farciminis on the restoration of stress-induced mucin O-glycosylation changes was also shown, and the possible effect of L. farciminis on glycosylation enzymes through its anti-inflammatory profile was underlined [18]. Interestingly, the capsular polysaccharide production by the probiotic strain L. farciminis CNCM I-3699 was established [19]. Despite the significant clinical evidence for the health benefits associated with the consumption of L. farciminis CIP 103136, the molecular mechanisms underlying these health effects are still poorly described.…”

Section: Introductionmentioning

confidence: 99%

Structure and biological activities of a hexosamine-rich cell wall polysaccharide isolated from the probiotic Lactobacillus farciminis

et al. 2019

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Lactobacillus farciminis CIP 103136 is a bacterial strain with recognized probiotic properties.However, the mechanisms underlying such properties have only been partially elucidated. In this study, we isolated and purified a cell-wall associated polysaccharide (CWPS), and evaluated its biological role in vitro. The structure of CWPS and responses from stimulation of (i) human macrophage-like THP-1 cells, (ii) human embryonal kidney (HEK293) cells stably transfected with Toll-like receptors (TLR2 or TLR4) and (iii) human colonocyte-like T84 intestinal epithelial cells, upon exposure to CWPS were studied. The structure of the purified CWPS from L. farciminis CIP 103136 was analyzed by nuclear magnetic resonance (NMR), MALDI-TOF-TOF MS, and methylation analyses in its native form and following Smith degradation. It was shown to be a novel branched polysaccharide, composed of linear backbone of trisaccharide repeating units of:[→6αGlcpNAc1→4βManpNAc1→4βGlcpNAc1→] highly substituted with single residues of αGlcp, aGalp and αGlcpNAc. Subsequently, the lack of pro-or anti-inflammatory properties of CWPS was established on macrophage-like THP-1 cells. In addition, CWPS failed to modulate cell signaling pathways dependent of TLR2 and TLR4 in transfected HEK-cells. Finally, in T84 cells, CWPS neither influenced intestinal barrier integrity under basal conditions nor prevented TNF-a/IFN-g cytokinemediated epithelium impairment.

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Rough and smooth morphotypes isolated from Lactobacillus farciminis CNCM I-3699 are two closely-related variants

Cited by 9 publications

References 63 publications

Genome Sequence of Rough and Smooth Variants of Pleomorphic Strain Lactobacillus farciminis CNCM-I-3699

Genome Sequence of Rough and Smooth Variants of Pleomorphic Strain Lactobacillus farciminis CNCM-I-3699

Surface properties associated with the production of polysaccharides in the food bacteria Propionibacterium freudenreichii

Structure and biological activities of a hexosamine-rich cell wall polysaccharide isolated from the probiotic Lactobacillus farciminis

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