Routine assay of total urinary hydroxyproline based on resin-catalysed hydrolysis

Goverde, B. C.; Veenkamp, F.J.N.

doi:10.1016/0009-8981(72)90492-5

Cited by 93 publications

(20 citation statements)

References 21 publications

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“…Sodium, potassium, calcium, phosphate, chloride, iron, creatinine, uric acid, glutamate oxalate and glutamate pyruvate transaminases, alkaline phosphatase, cholesterol, bilirubin, c~-amylase, PAH, inulin, haemoglobin, erythrocyte and leucocyte counts, and blood sedimentation rate were measured by standard techniques in the University of Basel Hospital Laboratory. Urinary cyclic AMP was measured by RIA using commercial antibodies (Becton Dickinson, Orangeburg, NY), and hydroxyproline was analysed according to Goverde and Veenkamp [1972]). …”

Section: Methodsmentioning

confidence: 99%

Intracolonic bioavailability of human calcitonin in man

Beglinger

Born

Muff

et al. 1992

Eur J Clin Pharmacol

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Human calcitonin (hCT) injected into the lumen of the descending colon of normal human subjects was absorbed within minutes and could be recognized intact in plasma as shown by RIA in combination with reverse-phase HPLC. The absorption was low and variable, with bioavailabilities ranging from 0.01% to 2.7% relative to intravenously administered hCT (area under the concentration-time curve). With intravenous hCT serum calcium was lowered and the fractional urinary excretion of calcium, phosphorus, sodium and chloride was significantly stimulated. With the intracolonic hCT, the fractional urinary excretions of calcium, sodium and chloride were also marginally stimulated relative to intracolonic vehicle (placebo). In conclusion, hCT is absorbed intact from the colon, but the bioavailability is low and highly variable.

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Section: Methodsmentioning

confidence: 99%

Intracolonic bioavailability of human calcitonin in man

Beglinger

Born

Muff

et al. 1992

Eur J Clin Pharmacol

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“…Serum total and bone alkaline phosphatase (AP) were determined colorimetrically (Granutest 3, Merck Diagnostica, Darmstadt, Germany) and by electrophoresis (using Paragon reagents, Beckman Instruments, Munich, Germany), respectively. Urinary cyclic-AMP was measured by an inhouse protein-binding radioassay, urinary hydroxyproline colorimetrically [34]. Urinary pyridinoline (PYD) and deoxypyridinoline (DPD) were measured by high-pressure liquid chromatography using commercially available reagents (Immunodiagnostik, Bensheim, Germany).…”

Section: Analysesmentioning

confidence: 99%

High insulin and low IGF-I plasma levels following pancreas transplantation in rats. Implications for bone and mineral metabolism

Hofmann²,

Gepp³

et al. 2000

Scandinavian Journal of Clinical and Laboratory Investigation

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Primary disturbances in mineral metabolism and deficiencies in insulin and insulin-like growth factor-I (IGF-I) have been implicated in the pathogenesis of diabetic osteopenia. This prompted us to investigate whether normal bone minerals and bone morphology are preserved after pancreas transplantation. To this end, 8 inbred rats (transplants) were compared with 9 sham-operated rats (controls) 20 months after orthotopic pancreas transplantation. While basal levels of insulin remained unaffected by transplantation, an oral glucose load elicited hyperinsulinemia (integrated incremental response: mean +/- SEM, 62+/-8 nmol l(-1) 60 min in transplants vs. 32+/-6 nmol l(-1) 60 min in controls; p<0.01) in the presence of normal glucose levels. Fecal and urinary excretion and fractional intestinal absorption of calcium, magnesium and phosphorus, net calcium absorption and the respective serum mineral levels were unchanged after transplantation, as were those of the calciotropic hormones. Serum osteocalcin and bone alkaline phosphatase remained unaffected, and urinary excretion of pyridinium and deoxypyridinium were unchanged. Fasting plasma IGF-I concentration was significantly decreased in transplants (930+/-42 ng ml(-1)) vs. control rats (1074+/-49 ng ml(-1); p < 0.05). Despite similar physical and chemical properties of bone in both groups, histomorphometry revealed slight osteopenia in transplant rats, as reflected by a 38% reduction in the cancellous bone area of the proximal tibial metaphysis. Plasma IGF-I levels were significantly correlated with bone mineral apposition rate (r=0.70, p<0.02), osteoblast perimeter (r=0.60, p<0.05) and osteoid perimeter (r=0.60, p<0.05). In conclusion, pancreas transplantation preserves physical and chemical properties of bone, but bone metabolism is not completely normal after transplantation, as evidenced by decreased cancellous bone. This might have resulted from the insulin resistance associated with the lowering of the plasma IGF-I level, which was correlated with the mineral apposition rate.

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“…Magnesium was determined by AAS. Routine methods were used for the determination of phosphorus, serum alkaline phosphatase, total protein, urinary creatinine, hydroxyproline (22), and cyclic AMP (23). Hormones were determined by radoimmunoassay: in serum or plasma gastrin (24), bioinactive mid-regional (25) and bioactive NH2-terminal parathyroid hormone (PTH) (26), calcitonin (27), osteocalcin (28), and cholecystokinin (29).…”

Section: Analysesmentioning

confidence: 99%

Highly Selective Vagotomy in the Rat: Effects on Bone and Mineral Metabolism

Rümenapf

Schwille

Wagner

et al. 1994

Scandinavian Journal of Gastroenterology

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Highly selective vagotomy (HSV) or sham operation was performed in male rats. Fifteen weeks later bone mineralization, fractional intestinal absorption and balance, urinary excretion, and serum levels of calcium, magnesium and phosphorus, together with serum gastrin, parathyroid hormone, calcitonin, vitamin D metabolites, osteocalcin, isoenzymes of alkaline phosphatase, and the urinary excretion of cyclic AMP and hydroxyproline were assessed. HSV induced chronic hypergastrinemia and enhanced the weight of the fundus, antrum, and pancreas. Body weight, food intake, intestinal absorption, mineral balance, and bone mineralization were unaffected by HSV, whereas serum parathyroid hormone levels and urinary hydroxyproline excretion were increased. It is concluded that in the rat 1) HSV has a trophic effect on gastric and extragastric tissues; 2) gastric acid production is not a major determinant of intestinal calcium absorption; and 3) normal bone mass in the presence of signs of hyperparathyroidism indicates an intrinsic capacity of HSV to interfere with calcium metabolism, probably via hypergastrinemia, gastrin being an element of the gastro-parathyroid axis. Our present findings underscore the fact that osteopenia after HSV in man may be a rare finding, but it cannot be ruled out that bone disease found after partial or total gastrectomy may be due in part to concomitant vagotomy.

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Routine assay of total urinary hydroxyproline based on resin-catalysed hydrolysis

Cited by 93 publications

References 21 publications

Intracolonic bioavailability of human calcitonin in man

Intracolonic bioavailability of human calcitonin in man

High insulin and low IGF-I plasma levels following pancreas transplantation in rats. Implications for bone and mineral metabolism

Highly Selective Vagotomy in the Rat: Effects on Bone and Mineral Metabolism

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