Routine HLA‐B genotyping with PCR‐sequence‐specific oligonucleotides (PCR‐SSO) detects eight new alleles: B*0807, B*0809, B*1551, B*3529, B*3532, B*4025, B*5304 and B*5508

Abstract

This paper describes eight new alleles (B*0807, B*0809, B*1551, B*3529, B*3532, B*4025, B*5304 and B*5508) that have been found by routine HLA-B genotyping with sequence-specific oligonucleotides (SSOs). All of the new alleles have variations which cause changes in residues that occur within antigen binding pockets and T-cell recognition sites of the antigen. The new polymorphisms within these new alleles may affect the nature and specificity of peptide binding and cause differential T-cell activation, which may have an affect in transplantation.

Six new HLA Class I alleles detected by PCR‐SSO genotyping

“…The new alleles reported here are in addition to another eight new HLA‐B alleles reported earlier (4), which were detected by routine PCR‐SSO genotyping of HLA Class I genes. PCR‐SSO is a relatively non‐toxic, accurate, reproducible and reliable technique that is particularly suited for HLA Class I genotyping of donors to bone marrow registries or whenever typing a large sample cohort.…”

A novel HLA‐B allele, B*5612, identified by sequence‐based typing method

“…HLA‐B*5612 was detected in a candidate for bone marrow transplantation initially described as B*46 and B*5508 when using Micro‐SSP HLA class I typing tray (One Lambda, Canoga Park, CA). Since B*5508 allele was first reported in Australian Aboriginal (Kennedy et al ., 2000) and was never observed previously in Taiwan, this HLA‐B typing needed confirming. The new hybridization pattern obtained using the reverse line SSO typing kit (Dynal Biotech, Bromborough, Wirral, UK) revealed two additional positive probes (probe 28 (SHIIQR) and probe 50 (LEGTC)) and suggested that codon 92–97 and codon 160–164 were different from B*5508 (Fig.…”

Haplotype‐specific extraction: a universal method to resolve ambiguous genotypes and detect new alleles – demonstrated on HLA‐B

“…Current human leukocyte antigen (HLA) typing strategies detect HLA-B polymorphisms in genomic DNA with sequence-specific primers (SSP) (1,2), sequence-specific oligonucleotides (SSO) (3,4), or sequence-based (SB) typings (4,5). Polymorphisms are not assigned to paternal or maternal haplotypes.…”