Routine identification of microorganisms by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: Success rate, economic analysis, and clinical outcome

Ge, Mao-Cheng; Kuo, An-Jing; Liu, Kuei-Lan; Wen, Yu‐Chuan; Chia, Ju‐Hsin; Chang, Pi-Yueh; Lee, Ming-Hsun; Wu, Tsu‐Lan; Chang, Shih-Cheng; Lu, Jang‐Jih

doi:10.1016/j.jmii.2016.06.002

Cited by 73 publications

(55 citation statements)

References 23 publications

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“…An overall rate of more than 75% species identification using direct smear application (Bizzini et al, 2010;McElvania-TeKippe et al, 2013) is supported by the findings of the current study (93.5%). It is noteworthy that application of 70% formic acid as a layer on top of the colony material before applying matrix can increase the identification rate (McElvania-TeKippe et al, 2013;Ge et al, 2017). When a yeast infection is suspected, the direct smear approach is not sufficient, as described by Cassagne et al (2013); it requires the ethanol: formic acid: nitrile protocol.…”

Section: Discussionmentioning

confidence: 99%

Bovine mastitis bacteria resolved by MALDI-TOF mass spectrometry

Nonnemann

Lyhs

Svennesen

et al. 2019

Journal of Dairy Science

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Matrix-assisted laser desorption/ionization timeof-flight mass spectrometry (MALDI-TOF MS) is a fast and reliable method to identify the most common pathogenic bacteria in humans and animals. The goals of this study were to amend a commercial database with additional species, evaluate the amended database for identification of bacterial genera and species causing bovine mastitis, and describe the plethora of species involved. In total, 500 udder pathogenic isolates were subjected to MALDI-TOF MS using bacterial or fungal colony material; 93.5% could be identified to the species level, and 6.5% were identified only to the genus level. Isolates identified to the genus level required further identification to the species level by conventional methods or 16S rDNA sequencing. Mass spectra from verified species were used to expand the MALDI-TOF MS database to improve future identification ability. A total of 24 genera and 61 species were identified in this study. Identified isolates were mainly staphylococci, streptococci, Enterobacteriaceae, and coryneforme bacteria. In conclusion, MALDI-TOF MS is a powerful, rapid, and reliable technique to identify the most common microorganisms causing bovine mastitis, and the database can be continuously expanded and improved with additional species.

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Section: Discussionmentioning

confidence: 99%

Bovine mastitis bacteria resolved by MALDI-TOF mass spectrometry

Nonnemann

Lyhs

Svennesen

et al. 2019

Journal of Dairy Science

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“…Mass spectrometry identification of organisms has increased over time and is now a standard in many laboratories worldwide. Although the initial equipment cost may be high, the laboratory savings for organism identification has been reported at greater than 50% compared to that with conventional methods (35)(36)(37). Additionally, specific identification turnaround time for B. pseudomallei may be improved by 16 to 24 h given the current method involves phenotypic analysis.…”

Section: Discussionmentioning

confidence: 99%

Identification of Burkholderia pseudomallei by Use of the Vitek Mass Spectrometer

2019

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Burkholderia pseudomallei is the causative agent of melioidosis. This condition most often presents as pneumonia and bacteremia, with mortality rates of 9% to 70%. Therefore, early identification of this organism may aid in directing appropriate management. This study aimed to use the Vitek matrix-assisted laser desorption ionization–time of flight mass spectrometer to create a spectrum for the rapid identification of B. pseudomallei. Spectra from 85 isolate cultures were acquired using the Vitek mass spectrometer research mode. A SuperSpectrum was created using peak matching and subsequently activated for analysis of organism identification. All 85 isolates were correctly identified as B. pseudomallei. A total of 899 spectra were analyzed and demonstrated a specificity of 99.8%. Eighty-one clinical isolates were used, of which 10 were neuromelioidosis, and no discernible spectrum difference was appreciated. Spectrum acquisition from a single spot was only successful in 374/899 (41%) of isolates. This increased to 100% when 3 spots of the same extract were analyzed. The Vitek mass spectrometer can be used for the rapid identification of B. pseudomallei with a high level of specificity.

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“…La capacidad para analizar un gran número de aislamientos simultáneamente permite incrementar el rendimiento; además, una vez cargado el instrumento, las identificaciones se pueden realizar en <1 minuto 64 . Esta mejora en el tiempo de respuesta representa un beneficio sustancial en la oportunidad del resultado y la atención del paciente 65,66 , así como en el ahorro que genera en los costos de atención 33 . Sin embargo, la elección de esta tecnología conlleva costos importantes relacionados con la adquisición o arrendamiento del equipo, además de los costos de mantenimiento que han sido calculados de hasta un 10% del costo del equipo anualmente 67 .…”

Section: Impacto Del Uso Del Maldi Tof Msunclassified

“…Una de las características de MALDI-TOF MS es el menor costo de identificación por aislamiento comparado con el de otros sistemas 18,22,65,68 . Un estudio prospectivo que evaluó específicamente los aspectos de tiempo para detección y costo efectividad del uso de MALDI-TOF MS en un laboratorio hospitalario en 952 aislamientos clínicos, determinó que el MALDI-TOF MS realizó la identificación de microorganismos 1,45 días más temprano que la metodología estándar del laboratorio, y que la incorporación de esta tecnología permitió ahorrar 52% en costos laborales y reactivos invertidos en la identificación de microorganismos por año 66 .…”

Section: Impacto Del Uso Del Maldi Tof Msunclassified

La espectrometría de masas MALDI-TOF en el laboratorio de microbiología clínica

Maldonado

Robledo

2017

Infect

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ResumenLos métodos fenotípicos empleados para la identificación de microorganismos dependen de procesos metabólicos que requieren de tiempos de incubación mínimos para alcanzar resultados confiables. La espectrometría de masas MALDI-TOF (desorción/ionización láser asistida por una matriz con detección de masas por tiempo de vuelo) se ha instaurado como una metodología relevante para la identificación de microorganismos mediante el análisis de proteínas, a través de la creación de un espectro de masas específico de género y especie. En esta revisión, se presenta MALDI-TOF MS como una tecnología precisa para la identificación de bacterias, levaduras, mohos, en incluso de virus, que además, permite la reducción del tiempo para obtener un resultado de identificación, que puede impactar los costos de atención y duración de la estancia hospitalaria. La identificación de microorganismos directamente de muestras biológicas y la detección de mecanismos de resistencia a antimicrobianos, prometen un mayor impacto clínico y epidemiológico con el desarrollo e implementación de esta tecnología en los laboratorios de microbiología clínica.Palabras clave: MALDI-TOF MS, espectrometría de masas, diagnóstico microbiológico, identificación de patógenos, proteómica, infección del torrente sanguíneo, infección del tracto urinario. MALDI-TOF mass spectrometry in clinical microbiology laboratories. AbstractPhenotypic methods used for the identification of microorganisms depend on metabolic processes that require minimum incubation times to achieve reliable results. For this reason, MALDI-TOF MS (Matrix Assisted Laser Desorption/Ionization Mass Spectrometry) has been established as a relevant methodology for the identification of microorganisms using analysis of proteins, through the creation of a mass spectrum specific for genus and species. In the present review, MALDI-TOF MS is presented as an accurate technology for identifying bacteria, yeasts, molds and viruses; Its use allows reduction of the time to obtain an identification result, which may impact the costs of care and length of hospital stay. The identification of microorganisms directly from biological samples and the detection of mechanisms of antimicrobial resistance, promise an additional clinical and epidemiological impact with the development and implementation of this technology in clinical microbiology laboratories.Keywords: MALDI-TOF MS, mass spectrometry, microbiological diagnostic, pathogen identification, proteomics, blood stream infection, urinary tract infection. REVISIÓN IntroducciónLa identificación de microorganismos se ha realizado tradicionalmente por métodos basados en tinciones que permiten la clasificación de la morfología microscópica con el fin de apoyar decisiones diagnósticas y terapéuticas tempranas; así como en pruebas in vitro basadas en reacciones bioquímicas utilizadas por sistemas manuales o automatizados, que integran pruebas con diferentes sustratos, incrementando la rapidez y simplicidad de la identificación. Sin embargo, estos métodos fenotí...

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Routine identification of microorganisms by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: Success rate, economic analysis, and clinical outcome

Abstract: MALDI-TOF MS is a rapid, reliable, economical, and environmentally friendly method for routine microbial identification and may contribute to early appropriate antibiotic treatment in clinical settings.

Cited by 73 publications

References 23 publications

Bovine mastitis bacteria resolved by MALDI-TOF mass spectrometry

Bovine mastitis bacteria resolved by MALDI-TOF mass spectrometry

Identification of Burkholderia pseudomallei by Use of the Vitek Mass Spectrometer

La espectrometría de masas MALDI-TOF en el laboratorio de microbiología clínica

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