Routine measurement of amniotic fluid alpha-fetoprotein and acetylcholinesterase: the need for a reevaluation

Flick, Amy Alicia; Krakow, Deborah; Martirosian, Anahit; Silverman, Neil S.; Platt, Lawrence D.

doi:10.1016/j.ajog.2014.02.005

Cited by 31 publications

(19 citation statements)

References 23 publications

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“…Still, its predicative value is matter of discussion (6,7). To the best of our knowledge there is only scarce data available on AF-AFP concentration in amnion fluid during the course of pregnancy.…”

Section: Discussionmentioning

confidence: 99%

Alpha-fetoprotein and its value for predicting pregnancy outcomes � a re-evaluation

Darouich

Liehr

Weise

et al. 2015

JPM

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Introduction: Alpha-fetoprotein (AFP) concentrations can be determined framing others from invasively acquired amnion fluid (AF-AFP). While the biological role of AFP remains unclear it is well known that AFP-levels can be altered in connection with specific clinical and/or genetic alterations of the fetus. Materials and Method: here a retrospective study based on 3,119 singleton and 56 twin pregnancies is presented. The standard levels of amnion fluid derived alpha-fetoprotein level (AF-AFP) between 12th and 36th weeks of gestation were determined. Additionally, acetylcholinesterase (AChE) test results for 63 cases, ultrasonography results for 32 cases and abnormal karyotypic findings for 100 cases were available for selected cases. Results and Discussion: according to the present data the AF-AFP test is reliable and provides ex-

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Section: Discussionmentioning

confidence: 99%

Alpha-fetoprotein and its value for predicting pregnancy outcomes � a re-evaluation

Darouich

Liehr

Weise

et al. 2015

JPM

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“…Our findings indicate that this model has higher sensitivity and specificity for screening NTDs than AFP. In clinical practice, amniotic fluid AFP testing with the inclusion of acetylcholinesterase, which is measured if the AFP levels are abnormally high, has the sensitivity of 22% to 77% (Wang et al 2009;Flick et al 2014) and the specificity of about 97.7% (Wang et al 2009). …”

Section: Discussionmentioning

confidence: 99%

“…In addition, the levels of AFP in the amniotic fluid and maternal serum can be normal if the spina bifida is covered with healthy skin. As mentioned above, AFP screening suffers from having poor sensitivity (Wang et al 2009;Flick et al 2014). Ultrasound is used both as a screening test and as a follow-up test after a positive result of AFP screening.…”

Section: Discussionmentioning

confidence: 99%

Identification of Biomarkers by Proteomics for Prenatal Screening for Neural Tube Defects

Shen

et al. 2016

Tohoku J. Exp. Med.

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Neural tube defect (NTD) is a serious congenital defect, but current methods for identifying NTD are limited. We used proteomic analysis of maternal serum to identify NTD-specific proteins whose levels differed between women with NTD fetuses (n = 50) and those with healthy fetuses (n = 40). Three NTD-specific protein peaks (8,130.6, 15,941.7, and 3,960.3 m/z) were identified using MALDI-TOF-mass spectrophotemetry, and were included in a diagnostic model developed using Biomarker Patterns software. The model used cut-offs for the relative intensity of the three peaks to indicate if a case had or did not have NTD. The model identified 48 of the 50 NTD cases and 36 of the 40 control cases correctly, resulting in the sensitivity of 96.0% (48/50) and the specificity of 90.0% (36/40). The diagnostic model was also tested on 105 clinical cases at high risk for NTD, as determined by having high alpha-fetoprotein levels, resulting in the sensitivity of 100% (101/101) and the specificity of 75.0% (3/4). Using the International Protein Index database, we identified proteins with a molecular mass of 8,130.6 Da as ADP-ribosylation factor 1 and a protein similar to cold agglutinin FS-1 antibody light-chain. The 15,941.7-Da peak corresponded to vitamin K3 protein, and the identity of the 3,960.3-Da protein was unclear. Thus, this study developed a diagnostic model consisting of the three peaks which may be indicators of NTD. This new assay may be at least as accurate for diagnosing NTD compared with the commonly used clinical test that assesses alphafetoprotein levels.

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“…Maternal AFP nöral tüp defekleri ve karın duvarı defektlerinde yükselir. Bu hastalarda amniotik sıvıda AFP ve asetilkolinesteraz seviyeleri bakılması yol gösterici olabilir (81) .…”

Section: Taniunclassified

Abdominal wall defects: omphalocele and gastrschisis

Yazıcı¹

2017

tcdd

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Routine measurement of amniotic fluid alpha-fetoprotein and acetylcholinesterase: the need for a reevaluation

Cited by 31 publications

References 23 publications

Alpha-fetoprotein and its value for predicting pregnancy outcomes � a re-evaluation

Alpha-fetoprotein and its value for predicting pregnancy outcomes � a re-evaluation

Identification of Biomarkers by Proteomics for Prenatal Screening for Neural Tube Defects

Abdominal wall defects: omphalocele and gastrschisis

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